CCL2: An important cytokine in normal and pathological pregnancies: A review

Lin, Zhiang; Shi, Jialu; Chen, Min; Zheng, Zi‐Meng; Li, Ming‐Qing; Shao, Jun

doi:10.3389/fimmu.2022.1053457

Cited by 26 publications

(20 citation statements)

References 155 publications

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“…Among them, CCL2 was reported to be expressed in trophoblasts and decidual tissue at the maternal-fetal interface and proposed to contribute to immune cell recruitment, tissue remodeling, and angiogenesis during placentation. 29 On the other hand, it was also revealed that the spheroid-derived Swan71 cells increased the expressions of C-X-C motif chemokines, CXCL1, CXCL2, CXCL5, CXCL6, CXCL8, and CXCL10. A recent study indicated that CXCL1 is necessary for decidual angiogenesis during the first trimester.…”

Section: Discussionmentioning

confidence: 97%

“…This study showed that the expressions of C‐C motif chemokines, CCL2, CCL7, and CCL20, were increased in the spheroid‐derived Swan71 cells. Among them, CCL2 was reported to be expressed in trophoblasts and decidual tissue at the maternal‐fetal interface and proposed to contribute to immune cell recruitment, tissue remodeling, and angiogenesis during placentation 29 . On the other hand, it was also revealed that the spheroid‐derived Swan71 cells increased the expressions of C‐X‐C motif chemokines, CXCL1, CXCL2, CXCL5, CXCL6, CXCL8, and CXCL10.…”

Section: Discussionmentioning

confidence: 99%

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Spheroid formation induces chemokine production in trophoblast‐derived Swan71 cells

Kanda

Kagami

Iizuka

et al. 2023

American J Rep Immunol

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Problem: In the cell column of anchoring villi, the cytotrophoblast differentiates into extravillous trophoblast (EVT) and invades the endometrium in contact with maternal immune cells. Recently, chemokines were proposed to regulate the decidual immune response. To investigate the roles of chemokines around the anchoring villi, we examined the expression profiles of chemokines in the first-trimester trophoblast-derived Swan71 cells using a three-dimensional culture model. Method of Study:The gene expressions in the spheroid-formed Swan71 cells were examined by microarray and qPCR analyses. The protein expressions were examined by immunochemical staining. The chemoattractant effects of spheroid-formed Swan71 cells were examined by migration assay using monocyte-derived THP-1 cells. Results:The expressions of an EVT marker, laeverin, and matrix metalloproteases, MMP2 and MMP9, were increased in the spheroid-cultured Swan71 cells. Microarray and qPCR analysis revealed that mRNA expressions of various chemokines, CCL2, CCL7, CCL20, CXCL1, CXCL2, CXCL5, CXCL6, CXCL8, and CXCL10, in the spheroid-cultured Swan71 cells were up-regulated as compared with those in the monolayer-cultured Swan71 cells. These expressions were significantly suppressed by hypoxia. Migration assay showed that culture media derived from the spheroid-formed Swan71 cells promoted THP-1 cell migration. Conclusion:This study indicated that chemokine expressions in Swan71 cells increase under a spheroid-forming culture and the culture media have chemoattractant effects.Since three-dimensional cell assembling in the spheroid resembles the structure of the cell column, this study also suggests that chemokines play important roles in the interaction between EVT and immune cells in their early differentiation stage.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Spheroid formation induces chemokine production in trophoblast‐derived Swan71 cells

Kanda

Kagami

Iizuka

et al. 2023

American J Rep Immunol

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“…Notably, we also found that other inflammatory factors such as MCP‐1, also known as CCL2, increased in spleen lysate, BM lysate and serum at day 1. Since CCL2 is an integral chemotactic factor known to play a crucial role in recruiting monocytes from the bone marrow to various tissues including the spleen, in response to inflammation or injury (Lin et al , 2022), it may also recruit the myeloid cells to the inflammation site during acute phase post A. m . stimulation.…”

Section: Discussionmentioning

confidence: 99%

“…Notably, we also found that other inflammatory factors such as MCP-1, also known as CCL2, increased in spleen lysate, BM lysate and serum at day 1. Since CCL2 is an integral chemotactic factor known to play a crucial role in recruiting monocytes from the bone marrow to various tissues including the spleen, in response to inflammation or injury (Lin et al, 2022), it may also recruit the myeloid cells to the inflammation site during acute phase post A. m. stimulation. We found that late-onset delayed EMH in the spleen is mediated by innate immune signals, particularly TLR2/4 and IL-1R, with the latter possibly being regulated by local and persistent secretion of IL-1a upon A. m. stimulation.…”

Section: Discussionmentioning

confidence: 99%

Akkermansia muciniphila induces slow extramedullary hematopoiesis via cooperative IL‐1R/TLR signals

Wang,

Morishima,

Sezaki

et al. 2023

EMBO Reports

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Bacterial infections can activate and mobilize hematopoietic stem and progenitor cells (HSPCs) from the bone marrow (BM) to the spleen, a process termed extramedullary hematopoiesis (EMH). Recent studies suggest that commensal bacteria regulate not only the host immune system but also hematopoietic homeostasis. However, the impact of gut microbes on hematopoietic pathology remains unclear. Here, we find that systemic single injections of Akkermansia muciniphila (A. m.), a mucin‐degrading bacterium, rapidly activate BM myelopoiesis and slow but long‐lasting hepato‐splenomegaly, characterized by the expansion and differentiation of functional HSPCs, which we term delayed EMH. Mechanistically, delayed EMH triggered by A. m. is mediated entirely by the MYD88/TRIF innate immune signaling pathway, which persistently stimulates splenic myeloid cells to secrete interleukin (IL)‐1α, and in turn, activates IL‐1 receptor (IL‐1R)‐expressing splenic HSPCs. Genetic deletion of Toll‐like receptor‐2 and ‐4 (TLR2/4) or IL‐1α partially diminishes A. m.‐induced delayed EMH, while inhibition of both pathways alleviates splenomegaly and EMH. Our results demonstrate that cooperative IL‐1R‐ and TLR‐mediated signals regulate commensal bacteria‐driven EMH, which might be relevant for certain autoimmune disorders.

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“…We found that the compound was not able to reduce the secretion of TNF-α, an important cytokine involved in triggering the innate immune response against host invaders [27,28]. The inhibited cytokines are responsible for different biological effects, contributing to the development and progression of inflammatory responses and increasing the response of cells involved in the immune system [29][30][31][32]. Similarly, other studies have already shown the ability of different 1,4-DHP derivatives to inhibit the production of these same pro-inflammatory cytokines in different experimental models, and as in our experiments, the 1,4-DHP derivatives did not reduce TNF-α levels [10,11,33].…”

Section: Groupmentioning

confidence: 97%

Evaluation of the anti‐inflammatory effect of 1,4‐dihydropyridine derivatives

Facchin,

Lubschinski,

Moon

et al. 2023

Fundamemntal Clinical Pharma

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IntroductionInflammation is a physiological event that protects the organism against different factors that lead to loss of tissue homeostasis. Dihydropyridine (DHP) derivatives are heterocyclic compounds known for their different biological activities, including anti‐inflammatory activities.ObjectiveTo evaluate the anti‐inflammatory activity of 1,4‐dihydropyridine (1,4‐DHP) derivatives using anti‐inflammatory models in vitro, in RAW264.7 cells induced by lipopolysaccharide (LPS) and in vivo using the acute lung injury (ALI) model in mice.ResultsFifteen compounds derived from 1,4‐DHP were tested in RAW264.7 cells for their cytotoxic effect and cell viability. Thereafter, only the six compounds that showed the highest cell viability were tested for the production or inhibition of the pro‐inflammatory cytokine interleukin 6 (IL‐6). The best compound (compound 4) was tested for its anti‐inflammatory effects in vitro and in vivo, showing inhibition of nitric oxide (NO), pro‐inflammatory cytokines, increased phagocytic activity, and an increase in IL‐10 in vitro. In in vivo tests, compound 4 also reduces the levels of NO, myeloperoxidase (MPO) activity, leukocyte migration, and exudation, as well as reducing the levels of tumor necrosis factor‐alpha (TNF‐α) and IL‐6 and preventing the loss in the lung architecture.ConclusionThis compound showed important anti‐inflammatory activity, with a significant ability to reduce the production of pro‐inflammatory mediators and increase the phagocytic activity of macrophages and anti‐inflammatory mediator secretion (IL‐10). These findings led us to hypothesize that this compound can repolarize the macrophage response to an anti‐inflammatory profile (M2). Moreover, it was also able to maintain its anti‐inflammatory activity in vivo experiments.

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CCL2: An important cytokine in normal and pathological pregnancies: A review

Cited by 26 publications

References 155 publications

Spheroid formation induces chemokine production in trophoblast‐derived Swan71 cells

Spheroid formation induces chemokine production in trophoblast‐derived Swan71 cells

Akkermansia muciniphila induces slow extramedullary hematopoiesis via cooperative IL‐1R/TLR signals

Evaluation of the anti‐inflammatory effect of 1,4‐dihydropyridine derivatives

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