CCAAT/enhancer binding protein β-deficiency enhances type 1 diabetic bone phenotype by increasing marrow adiposity and bone resorption

Abstract

Motyl KJ, Raetz M, Tekalur SA, Schwartz RC, McCabe LR. CCAAT/enhancer binding protein ␤-deficiency enhances type 1 diabetic bone phenotype by increasing marrow adiposity and bone resorption.

Instruction of mesenchymal cell fate by the transcription factor C/EBPβ

“…Osteoblasts of C/EBPβ knockout mice displayed impaired differentiation and functional deficiency (Tominaga et al, 2008;Smink et al, 2009), including both cell autonomous and noncell autonomous mechanisms (Smink et al, 2009;Zanotti et al, 2009). Moreover, type 1 diabetes-induced bone mass reduction is further diminished in C/EBPβ-deficient mice and diabetic C/EBPβ-deficient mice displayed an enhanced adiposity in the bone marrow (Motyl et al, 2011). However, in this study no difference in bone formation parameters was shown.…”

Osseointegration of chitosan coated porous titanium alloy implant by reactive oxygen species-mediated activation of the PI3K/AKT pathway under diabetic conditions

“…Osteoblasts between the second and the fourth passages were used in all experiments to be seeded on the implants with a density of 1 Â 10 6 cells/ml and randomized to incubate with the following factors respectively: (1) TI treated with normal serum (NS); (2) TI treated with diabetic serum (DS); (3) CTI treated with DS; (4) TI treated with DS þ N-acetyl-L-cysteine (NAC, a ROS inhibitor, 20 mmol/L; Sigma, MO); (5) CTI treated with DS þ LY294002 (a PI3K inhibitor, 20 mmol/L; Sigma, MO). The diabetic mice were induced by intravenous injection of STZ (40 mg/kg, Sigma, MO) for 5 consecutive days as described previously [27] and confirmed with blood glucose greater than 300 mg/dl 15 days post-first injection. The DS was acquired from diabetic rats.…”

Role of Exercise in the Activation of Brown Adipose Tissue

“…This finding concurs with another study that reported lower bone mineral density and BAT mass in women with anorexia nervosa [44] . Interestingly, studies conducted in animal models with defective brown adipogenesis also showed a reduced bone mass [45,46] . Intervention studies are warranted to elucidate whether BAT activation induces improvement in bone mineral density, and whether BAT can be used as an anti-osteopenia and osteoporosis treatment.…”