CC-type glutaredoxins recruit the transcriptional co-repressor TOPLESS to TGA-dependent target promoters in Arabidopsis thaliana

Uhrig, Joachim F.; Huang, Lijun; Barghahn, Sina; Willmer, Moritz; Thurow, Corinna; Gatz, Christiane

doi:10.1016/j.bbagrm.2016.11.001

Cited by 50 publications

(66 citation statements)

References 44 publications

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“…We have shown previously that 20 out of the 21 ROXYs, including ROXY9, interacted with TGA2 in the yeast two‐hybrid system (Uhrig et al ., ). Likewise, ROXY9 interacted with TGA1 and TGA4 in yeast (Fig.…”

Section: Resultsmentioning

confidence: 97%

“…Since the pull‐down experiment had suggested similar affinities of ROXY19/GRX480 to TGA1 and TGA2 (Fig. S11b), we speculated that recruitment of the transcriptional repressor TOPLESS to the GBD‐ROXY19/AD‐TGA2 complex might interfere with transcriptional activation (Uhrig et al ., ). Indeed, when performing the assay with GBD‐ROXY19(L137A), which cannot bind TOPLESS due to the mutation of the ALWL motif to the ALWA sequence, luciferase activities increased by a factor of eight (Fig.…”

Section: Resultsmentioning

confidence: 97%

“…So far, it has remained unknown whether these CC-type GRXs exert similar functions as the other GRXs and/or whether they have adopted completely novel mechanisms of action (Gutsche et al, 2015). In Arabidopsis thaliana, CC-type GRXs (also called ROXYs) form a family of 21 genes and all but one interact with the bZIP transcription factor TGACG-BINDING FACTOR2 (TGA2) (Uhrig et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…A distinguished feature of those ROXYs that repress the activities of PAN and TGA2 is a functionally important conserved amino acid motif (A(L/I)W(L/V)) at the very end of the C terminus, which we call ALWL motif from here on. This ALWL motif mediates the interaction with the transcriptional co-repressor TOPLESS (Uhrig et al, 2016) suggesting that ALWL-containing ROXYs initiate the formation of a repressive complex in TGAregulated promoters. Interestingly, ROXYs containing the ALWL motif can functionally replace each other: when expressed under the ROXY1 promoter, ROXYs 2-5, 18 and 19/GRX480 can complement the roxy1 phenotype (Li et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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TGACG‐BINDING FACTORs (TGAs) and TGA‐interacting CC‐type glutaredoxins modulate hyponastic growth in Arabidopsis thaliana

Muthreich

Huang

et al. 2018

New Phytologist

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Summary TGACG‐BINDING FACTORs (TGAs) control the developmental or defense‐related processes. In Arabidopsis thaliana, the functions of at least TGA2 and PERIANTHIA (PAN) can be repressed by interacting with CC‐type glutaredoxins, which have the potential to control the redox state of target proteins. As TGA1 can be redox modulated in planta, we analyzed whether some of the 21 CC‐type glutaredoxins (ROXYs) encoded in the Arabidopsis genome can influence TGA1 activity in planta and whether the redox active cysteines of TGA1 are functionally important. We show that the tga1 tga4 mutant and plants ectopically expressing ROXY8 or ROXY9 are impaired in hyponastic growth. As expression of ROXY8 and ROXY9 is activated upon transfer of plants from hyponasty‐inducing low light to normal light, they might interfere with the growth‐promoting function of TGA1/TGA4 to facilitate reversal of hyponastic growth. The redox‐sensitive cysteines of TGA1 are not required for induction or reversal of hyponastic growth. TGA1 and TGA4 interact with ROXYs 8, 9, 18, and 19/GRX480, but ectopically expressed ROXY18 and ROXY19/GRX480 do not interfere with hyponastic growth. Our results therefore demonstrate functional specificities of individual ROXYs for distinct TGAs despite promiscuous protein–protein interactions and point to different repression mechanisms, depending on the TGA/ROXY combination.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

TGACG‐BINDING FACTORs (TGAs) and TGA‐interacting CC‐type glutaredoxins modulate hyponastic growth in Arabidopsis thaliana

Muthreich

Huang

et al. 2018

New Phytologist

Self Cite

View full text Add to dashboard Cite

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“…ROXY19 from A. thaliana interacts with TGA2/5/6, but a redox‐sensitive TGA2/5/6 DNA binding has thus far not been reported as a mechanism to regulate TGA2/5/6 activities in stress responses (Herrera‐Vasquez et al., ; Zander et al., ). ROXY19 might rather affect the TGA2 activity via its interaction with TOPLESS, a transcriptional corepressor (Uhrig et al., ). However, as ROXY19 can complement the roxy1 petal mutant phenotype (Li et al., ), it might thus also be capable to interact together with PAN at regulatory DNA elements, given it is expressed in a floral context.…”

Section: Discussionmentioning

confidence: 99%

Conserved redox‐dependent DNA binding of ROXY glutaredoxins with TGA transcription factors

et al. 2017

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The Arabidopsis thaliana CC-type glutaredoxin (GRX) ROXY1 and the bZIP TGA transcription factor (TF) PERIANTHIA (PAN) interact in the nucleus and together regulate petal development. The CC-type GRXs exist exclusively in land plants, and in contrast to the ubiquitously occurring CPYC and CGFS GRX classes, only the CCtype GRXs expanded strongly during land plant evolution. Phylogenetic analyses show that TGA TFs evolved before the CC-type GRXs in charophycean algae.

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The AP2/ERF transcription factor ORA59 regulates ethylene‐induced phytoalexin synthesis through modulation of an acyltransferase gene expression

Huang

Zhang

Zeng

et al. 2022

Journal Cellular Physiology

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The gaseous ethylene (ET) and the oxylipin‐derived jasmonic acid (JA) in plants jointly regulate an arsenal of pathogen responsive genes involved in defending against necrotrophic pathogens. The APETALA2 (AP2)/ETHYLENE RESPONSE FACTOR (ERF) transcription factor ORA59 is a major positive regulator of the ET/JA‐mediated defense pathway in Arabidopsis thaliana. The Arabidopsis agmatine coumaroyltransferase (AtACT) catalyzes the formation of hydroxycinnamic acid amides (HCAAs) which are effective toxic antimicrobial substances known as phytoalexins and play an important role in plant defense response. However, induction and regulation of AtACT gene expression and HCAAs synthesis in plants remain less understood. Through gene coexpression network analysis, we identified a list of GCC‐box cis‐element containing genes that were coexpressed with ORA59 under diverse biotic stress conditions and might be potential downstream targets of this AP2/ERF‐domain transcription factor. Particularly, ORA59 directly binds to AtACT gene promoter via the GCC‐boxes and activates AtACT gene expression. The ET precursor 1‐aminocyclopropane‐1‐carboxylic acid (ACC)‐treatment significantly induces AtACT gene expression. Both ORA59 and members of the class II TGA transcription factors are indispensable for ACC‐induced AtACT expression. Interestingly, the expression of AtACT is also subject to the signaling crosstalk of the salicylic acid‐ and ET/JA‐mediated defense response pathways. In addition, we found that genes of the phenylpropanoid metabolism pathway were specifically induced by Botrytis cinerea. Taking together, these evidence suggest that the ET/JA signaling pathway activate the expression of AtACT to increase antimicrobial HCAAs production through the transcription factor ORA59 in response to the infection of necrotrophic plant pathogens.

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CC-type glutaredoxins recruit the transcriptional co-repressor TOPLESS to TGA-dependent target promoters in Arabidopsis thaliana

Cited by 50 publications

References 44 publications

TGACG‐BINDING FACTORs (TGAs) and TGA‐interacting CC‐type glutaredoxins modulate hyponastic growth in Arabidopsis thaliana

TGACG‐BINDING FACTORs (TGAs) and TGA‐interacting CC‐type glutaredoxins modulate hyponastic growth in Arabidopsis thaliana

Conserved redox‐dependent DNA binding of ROXY glutaredoxins with TGA transcription factors

The AP2/ERF transcription factor ORA59 regulates ethylene‐induced phytoalexin synthesis through modulation of an acyltransferase gene expression

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