Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development

Chao, Qinghe; Shen, Fengfeng; Xue, Yidong; Wu, Jianping; Zhang, Junling

doi:10.3390/ijms21041288

Cited by 8 publications

(5 citation statements)

References 35 publications

(37 reference statements)

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“…According to a previous study, CBX2 is a key member of the polycomb group (PcG) family and CBX2 mRNA was predominately localized in spermatogonia and spermatocytes, as demonstrated by in situ hybridization (ISH) (25). Tatavosian et al found that CBX2 undergoes phase separation to form condensates and that these CBX2 condensates concentrate DNA and nucleosomes (26).…”

Section: Discussionmentioning

confidence: 99%

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Prognosis and Immune Infiltration of Chromobox Family Genes in Sarcoma

et al. 2021

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BackgroundChromobox family genes (CBXs) are known to play roles in numerous modifications of the chromatin in order to inhibit the transcription of target genes. CBXs have been shown to be expressed at high levels in many types of cancer and can also serve as a target gene for therapeutic purposes. However, little is known about the expression and prognostic value of CBXs in human sarcomas.MethodsThe transcription level of CBXs was analyzed using the Oncomine dataset, and the differential expression of CBXs in sarcoma was reported by the Gene Expression Profiling Interactive Analysis (GEPIA) dataset. We also used the CCLE dataset to evaluate the expression of CBXs in a sarcoma cell line. The prognostic value of CBXs was analyzed using GEPIA and Kaplan–Meier analysis. In addition, the corrections between CBXs and their co-expressed genes were reported using Oncomine and GEPIA datasets. DAVID was used to perform GO function enrichment analysis for the CBXs and their co-expression genes. Finally, TIMER was used to analyze the immune cell infiltration of CBXs in patients with sarcoma.ResultsHP1-α/β/γ (CBX1/3/5) and CBX4/6/8 were found to be overexpressed in human sarcoma, and CBXs were upregulated in almost all the sarcoma cell line. The expression levels of HP1-α/β/γ (CBX1/3/5) and CBX7 were associated with overall survival (OS) in patients with sarcoma, while high expression levels of CBX7 were related to disease-free survival (DFS). In addition, the expression levels of CBX2/6/7 were related to recurrence-free survival (RFS). We also found that the CBX family was positively correlated with the infiltration of immune cells, including CD8+ T cells, CD4+ T cells, B cells, macrophages, neutrophils, and dendritic cells, in sarcoma.ConclusionsThe results from the present study indicated that CBXs were significantly associated with prognosis and immunological status in sarcoma. These data suggest that CBXs could serve as potential biomarkers for prognosis and immune infiltration in human sarcoma.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Prognosis and Immune Infiltration of Chromobox Family Genes in Sarcoma

et al. 2021

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“…In various vertebrates, dozens of effector pathways and genes involved in sex determination and gonadal development have been identified, including pathways involved in the Sox [ 33 ], Dmrt [ 34 ], Hox [ 35 ], and Wnt [ 36 ] gene families, as well as genes such as Sox9, Sox3, DAX1, AMH, Dmrt1, and Fgf9. The current research has demonstrated that gonadogenesis and development in fish are also co-regulated by these genes [ 37 , 38 ]. We performed a functional analysis of the DEGs, based on the known sequences in the database, and discovered that the DEGs were significantly enriched in the “reproductive process”, “ RNA modification”, “amide biosynthetic process”, “lipid metabolic process”, and “peptide transport processes”.…”

Section: Discussionmentioning

confidence: 99%

Gonadal Transcriptome Analysis and Sequence Characterization of Sex-Related Genes in Cranoglanis bouderius

Wang

Pan

Wang

et al. 2022

IJMS

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In China, the Cranoglanis bouderius is classified as a national class II-protected animal. The development of C. bouderius populations has been affected by a variety of factors over the past few decades, with severe declines occurring. Considering the likelihood of continued population declines of the C. bouderius in the future, it is critical to investigate the currently unknown characteristics of gonadal differentiation and sex-related genes for C. bouderius conservation. In this study, the Illumina sequencing platform was used to sequence the gonadal transcriptome of the C. bouderius to identify the pathways and genes related to gonadal development and analyze the expression differences in the gonads. A total of 12,002 DEGs were identified, with 7220 being significantly expressed in the ovary and 4782 being significantly expressed in the testis. According to the functional enrichment results, the cell cycle, RNA transport, apoptosis, Wnt signaling pathway, p53 signaling pathway, and prolactin signaling pathway play important roles in sex development in the C. bouderius. Furthermore, the sequence characterization and evolutionary analysis revealed that AMH, DAX1, NANOS1, and AR of the C. bouderius are highly conserved. Specifically, the qRT-PCR results from various tissues showed significant differences in AMH, DAX1, NANOS1, and AR expression levels in the gonads of both sexes of C. bouderius. These analyses indicated that AMH, DAX1, NANOS1, and AR may play important roles in the differentiation and development of C. bouderius gonads. To our best knowledge, this study is the first to analyze the C. bouderius gonadal transcriptome and identify the structures of sex-related genes, laying the foundation for future research.

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“…The CBX2 gene belongs to the Polycomb group (PcG) of protein family, which is involved in various biological processes, such as cell cycle regulation, cell differentiation, cell senescence, and X chromosome inactivation ( Mao et al, 2019 ; Plys et al, 2019 ). Previous research has indicated that CBX2 was an epigenetically modified transcription repressor ( Chao et al, 2020 ). Recent studies have linked high CBX2 expression with the poor prognosis of HCC patients.…”

Section: Discussionmentioning

confidence: 99%

Identification and verification of a novel epigenetic-related gene signature for predicting the prognosis of hepatocellular carcinoma

et al. 2022

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Hepatocellular carcinoma (HCC) is a common malignant tumor with a poor prognosis. Epigenetic dysregulation is now considered to be related to hepatocarcinogenesis. However, it is unclear how epigenetic-related genes (ERGs) contribute to the prognosis of HCC. In this study, we used the TCGA database to identify prognostic ERGs that were differentially expressed in HCC patients. Then, using least absolute shrinkage and selection operator (LASSO) regression analysis, a six-gene signature was constructed, and patients were divided into high- and low-risk groups. Validation was performed on HCC patients from the ICGC database. Patients in the high-risk group had a significantly lower chance of survival than those in the low-risk group (p < 0.001 in both databases). The predictive ability of the signature was determined by the receiver operating characteristic (ROC) curve. The risk score was then shown to be an independent prognostic factor for the overall survival (OS) of HCC patients based on the results of univariate and multivariate analyses. We also created a practical nomogram combining the prognostic model with other clinical features. Moreover, functional enrichment analysis revealed that these genes are linked to tumor immunity. In conclusion, our findings showed that a novel six-gene signature related to epigenetics can accurately predict the occurrence and prognosis of HCC.

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Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development

Cited by 8 publications

References 35 publications

Prognosis and Immune Infiltration of Chromobox Family Genes in Sarcoma

Prognosis and Immune Infiltration of Chromobox Family Genes in Sarcoma

Gonadal Transcriptome Analysis and Sequence Characterization of Sex-Related Genes in Cranoglanis bouderius

Identification and verification of a novel epigenetic-related gene signature for predicting the prognosis of hepatocellular carcinoma

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