Cbfa1/Runx2 gene expression in articular chondrocytes of the mice temporomandibular and knee joints in vivo

Kuboki, Takuo; Kanyama, Manabu; Nakanishi, Tohru; Akiyama, Kentaro; Nawachi, Kumiko; Yatani, Hirofumi; Yamashita, Kazuhito; Takano‐Yamamoto, Teruko; Takigawa, Masaharu

doi:10.1016/s0003-9969(03)00088-8

Cited by 15 publications

(17 citation statements)

References 20 publications

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“…5,6 Runx2/ Cbfa1, a transcription factor with a runt domain, is expressed in prehypertrophic and hypertrophic chondrocytes in articular tissues. 7 Runx2 promotes hypertrophic maturation of chondrocytes 8,9 followed by osteogenic processes. In contrast to the understanding of chondrocyte differentiation, the molecular mechanisms underlying joint formation are not fully elucidated.…”

Section: M O L E C U L E S R E G U L a T I N G A R T I C U L A R C mentioning

confidence: 99%

Molecular network of cartilage homeostasis and osteoarthritis

Hashimoto

Nakasa

Hikata

et al. 2007

Medicinal Research Reviews

103

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This review article presents the current understanding of the molecular basis of articular cartilaginous homeostasis, and outlines potential areas to focus on within the developing field of therapeutics for cartilage disorders. Articular cartilage, an integral component of joints in extremities and the vertebral column, is essential for locomotion. Disturbance of joint development or cartilage homeostasis causes congenital osteocartilaginous dysplasia or osteoarthritic diseases, respectively. Symptomatic treatments and surgical replacement of joints are effective but can also be problematic in terms of quality of life over time. Recently, new insights into the molecular biological basis of chondrocyte differentiation and cartilage homeostasis have been reported. While joint formation is regulated by several growth factors such as Wnts (wingless-related MMTV integration site) and Gdfs (growth and differentiation factors), the pathology of osteoarthritis is now interpreted as the disruption of balance between anabolic and catabolic signals. Current findings in molecular biology on joint development are reviewed concisely to aid in the understanding of the molecular network that governs articular cartilage development and homeostasis.

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Section: M O L E C U L E S R E G U L a T I N G A R T I C U L A R C mentioning

confidence: 99%

Molecular network of cartilage homeostasis and osteoarthritis

Hashimoto

Nakasa

Hikata

et al. 2007

Medicinal Research Reviews

103

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“…Cbfa1 expression has been seen in the articular surface of epiphyseal cartilage in the stifle joints of normal 3-week-old mice but not in 10-week-old mice, while it is strongly expressed in hypertrophic chondrocytes deep to the articulating layer of cartilage (Kuboki, Kanyama, Nakanishi, Akiyama, Nawachi, Yatani, Yamashita, Takano-Yamamoto and Takigawa 2003 …”

Section: 6 Cartilage Anlagenmentioning

confidence: 94%

“…The transcription factor core binding factor α1 (Cbfa1/Runx2, runt-related gene 2) is expressed by prehypertrophic and hypertrophic chondrocytes prior to cartilage mineralisation (Choi, Lee, Park, Bae, Shin, Nam, Kim, Kim and Ryoo 2002;Kuboki, Kanyama, Nakanishi, Akiyama, Nawachi, Yatani, Yamashita, Takano-Yamamoto and Takigawa 2003) and is essential for endochondral ossification. Cbfa1 expression has been seen in the articular surface of epiphyseal cartilage in the stifle joints of normal 3-week-old mice but not in 10-week-old mice, while it is strongly expressed in hypertrophic chondrocytes deep to the articulating layer of cartilage (Kuboki, Kanyama, Nakanishi, Akiyama, Nawachi, Yatani, Yamashita, Takano-Yamamoto and Takigawa 2003 …”

Section: 6 Cartilage Anlagenmentioning

confidence: 99%

Calcified tissue structure in the distal condyle of the third metacarpal bone in young Thoroughbred horses

Doube¹

2017

Preprint

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Aims: To determine improvements in third metacarpal (Mc3) condylar microanatomy attributable to preconditioning exercise. To investigate developmental causes of Mc3 condylar fracture.Methods: Twelve Thoroughbred horses were raised at pasture; six received preconditioning exercise from 10 days. Calcein labels were administered 19 and 11 days prior to euthanasia at 18 months. Six horses also received 2 seasons of race-training and were euthanised at 3 years. Slices were taken from the distal Mc3 condyle in the frontal and dorsal-and palmar-oblique frontal planes, scanned with DXA and macerated (frontal slices) or embedded in PMMA (oblique slices).Articular calcified cartilage (ACC) and subchondral bone (SCB) in oblique slices were imaged using confocal scanning light microscopy and quantitative backscattered electron scanning electron microscopy. ACC and SCB in the palmar slice lateral parasagittal grooves were imaged using µCT and nanoindentation tested. Results: Characteristic spatial variations in ACC and SCB histomorphometric parameters were present, none of which was significantly related to preconditioning exercise. Thickened, aberrantly mineralised ACC was found in 13/24 parasagittal grooves in the palmar slices and on the sagittal ridge of 4/12 dorsal slices of 18-month-old horses. Deep to thickened ACC, SCB had an open marrow structure, having not adopted the buttress morphology of the normal SCB plate. SCB in 3-year-old horses had incorporated early ACC defects as notches in parasagittal grooves and a hyaline cartilage island in a sagittal ridge. ACC was less stiff and SCB more stiff in affected than unaffected parasagittal grooves. Chondroclastic resorption in the parasagittal groove may be retarded as early as 3-6 months, possibly due to localised inhibition of Dr Naomi Boxall has guided me through the PhD experience; as one of the several PhD students 5 Attribution I knew while an undergraduate at Massey University she gave me insight into coping with the varied challenges that postgraduate study entails. Naomi also helped correct my sometimes dubious spelling, grammar and punctuation while preparing documents for submission. Jen Swindlehurst provided a number of images of equine metacarpals from her own studies that I was kindly allowed to use in my presentations. Jen's work also (conveniently) supported my own, and it was nice to have a colleague who was so intimately familiar with the problem of condylar fracture. Several of Professor

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“…Thus RUNX2 is just one of the many biological factors influencing the development and growth of condylar cartilage in humans and animals. RUNX2 expression was demonstrated and proof provided at the protein level in various studies on the prenatal and growing temporomandibular joint of the mouse and rat [47,70,[76][77][78].…”

Section: Runx2 and Jaw Growthmentioning

confidence: 99%

“…Zu den zahlreichen biologischen Faktoren, die die Entwicklung und das Wachstum des Kondylenknorpels bei Mensch und Tier beeinflussen, gehört daher auch RUNX2. In verschiedenen Studien am präna-talen und wachsenden Kiefergelenk der Maus und Ratte konnten sowohl die Expression gezeigt als auch der Nachweis auf Proteinebene geführt werden [47,70,[76][77][78]. were dissected, decalcified in EDTA and prepared for paraffin histology; serial sagittal sections were then produced.…”

Section: Runx2 Und Kieferwachstumunclassified

Die Bedeutung von RUNX2 in der postnatalen Entwicklung des Kondylus

et al. 2010

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We observed that RUNX2 is involved in normal condylar growth in the mouse and probably plays a significant role in osteogenesis and angiogenesis. The RUNX2 also has a biomechanical correlation in relation to cartilage compartmentalization. At the protein level, we noted no differences in the occurrence and distribution of RUNX2 in the condyle, except for a short phase during the 4th and 6th postnatal weeks, so that one allele might suffice for largely normal growth; other biological factors may have compensatory effects. However, we did observe small changes in a few cephalometric parameters concerning the mandibles of heterozygous knockout animals. We discuss potential correlations to our findings by relating them to the most current knowledge about the RUNX2 biology.

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Cbfa1/Runx2 gene expression in articular chondrocytes of the mice temporomandibular and knee joints in vivo

Cited by 15 publications

References 20 publications

Molecular network of cartilage homeostasis and osteoarthritis

Molecular network of cartilage homeostasis and osteoarthritis

Calcified tissue structure in the distal condyle of the third metacarpal bone in young Thoroughbred horses

Die Bedeutung von RUNX2 in der postnatalen Entwicklung des Kondylus

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