CAX1 suppresses Cd‐induced generation of reactive oxygen species in <i>Arabidopsis halleri</i>

Ahmadi, Hassan; Corso, Massimiliano; Weber, Michael; Verbruggen, Nathalie; Clemens, Stephan

doi:10.1111/pce.13362

Cited by 38 publications

(27 citation statements)

References 81 publications

(159 reference statements)

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“…Overexpression of the AhZIP6 copies in A. thaliana or their extinction in A. halleri altered cadmium tolerance but had no impact on zinc tolerance or the ionome of the plants, Text S1. A. thaliana transformation was performed by floral dip (Clough & Bent, 1998) and Agrobacterium tumefaciens (GV3101, pMP90) mediated stable transformation of A. halleri was performed using a tissue-culture based procedure (Ahmadi et al, 2018;Hanikenne et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

The two copies of the zinc and cadmiumZIP6transporter ofArabidopsis hallerihave distinct effects on cadmium tolerance

Spielmann

Ahmadi

Scheepers

et al. 2020

Plant Cell & Environment

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Plants have the ability to colonize highly diverse environments. The zinc and cadmium hyperaccumulator Arabidopsis halleri has adapted to establish populations on soils covering an extreme range of metal availabilities. The A. halleri ZIP6 gene presents several hallmarks of hyperaccumulation candidate genes: it is constitutively highly expressed in roots and shoots and is associated with a zinc accumulation quantitative trait locus. Here, we show that AhZIP6 is duplicated in the A. halleri genome. The two copies are expressed mainly in the vasculature in both A. halleri and Arabidopsis thaliana, indicative of conserved cis regulation, and acquired partial organ specialization. Yeast complementation assays determined that AhZIP6 is a zinc and cadmium transporter. AhZIP6 silencing in A. halleri or expression in A. thaliana alters cadmium tolerance, but has no impact on zinc and cadmium accumulation. AhZIP6silenced plants display reduced cadmium uptake upon short-term exposure, adding AhZIP6 to the limited number of Cd transporters supported by in planta evidence. Altogether, our data suggest that AhZIP6 is key to fine-tune metal homeostasis in specific cell types. This study additionally highlights the distinct fates of duplicated genes in A. halleri.

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Section: Discussionmentioning

confidence: 99%

The two copies of the zinc and cadmiumZIP6transporter ofArabidopsis hallerihave distinct effects on cadmium tolerance

Spielmann

Ahmadi

Scheepers

et al. 2020

Plant Cell & Environment

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“…Oxidative stress in plants occurs as a consequence of many abiotic stresses including extreme temperatures, drought, salinity, osmotic stress, and pollutants (e.g., heavy metals or herbicides) [1][2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

A Biostimulant Obtained from the Seaweed Ascophyllum nodosum Protects Arabidopsis thaliana from Severe Oxidative Stress

Omidbakhshfard

Sujeeth

Gupta

et al. 2020

IJMS

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Abiotic stresses cause oxidative damage in plants. Here, we demonstrate that foliar application of an extract from the seaweed Ascophyllum nodosum, SuperFifty (SF), largely prevents paraquat (PQ)-induced oxidative stress in Arabidopsis thaliana. While PQ-stressed plants develop necrotic lesions, plants pre-treated with SF (i.e., primed plants) were unaffected by PQ. Transcriptome analysis revealed induction of reactive oxygen species (ROS) marker genes, genes involved in ROS-induced programmed cell death, and autophagy-related genes after PQ treatment. These changes did not occur in PQ-stressed plants primed with SF. In contrast, upregulation of several carbohydrate metabolism genes, growth, and hormone signaling as well as antioxidant-related genes were specific to SF-primed plants. Metabolomic analyses revealed accumulation of the stress-protective metabolite maltose and the tricarboxylic acid cycle intermediates fumarate and malate in SF-primed plants. Lipidome analysis indicated that those lipids associated with oxidative stress-induced cell death and chloroplast degradation, such as triacylglycerols (TAGs), declined upon SF priming. Our study demonstrated that SF confers tolerance to PQ-induced oxidative stress in A. thaliana, an effect achieved by modulating a range of processes at the transcriptomic, metabolic, and lipid levels.

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“…halleri with its nonhyperaccumulating relative A rabidopsis lyrata ssp. petraea , quantitative trait loci (QTLs) for Zn accumulation (Willems et al, ), Zn tolerance (Willems et al, ), Cd accumulation, and Cd tolerance (Ahmadi, Corso, Weber, Verbruggen, & Clemens, ; Courbot et al, ) were detected. Second, comparative transcriptome studies with A .…”

Section: Introductionmentioning

confidence: 99%

“…First, in backcross populations derived from interspecific crosses of A. halleri with its nonhyperaccumulating relative Arabidopsis lyrata ssp. petraea, quantitative trait loci (QTLs) for Zn accumulation (Willems et al, 2010), Zn tolerance , Cd accumulation, and Cd tolerance (Ahmadi, Corso, Weber, Verbruggen, & Clemens, 2018;Courbot et al, 2007) were detected. Second, comparative transcriptome studies with A. halleri genotypes and Arabidopsis thaliana revealed that the transcripts of numerous metal homeostasis genes are constitutively more abundant in A. halleri (Becher, Talke, Krall, & Krämer, 2004;Talke, Hanikenne, & Krämer, 2006;Weber, Harada, Vess, von Roepenack-Lahaye, & Clemens, 2004).…”

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Elevated root nicotianamine concentrations are critical for Zn hyperaccumulation across diverse edaphic environments

Uraguchi

Weber

Clemens

2019

Plant Cell & Environment

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The metallophyte Arabidopsis halleri thrives across an extremely broad edaphic range. Zn hyperaccumulation is found on soils differing in available Zn by up to six orders of magnitude, raising the question as to whether a common set of mechanisms confers this species‐wide ability. Elevated root concentrations of the metal chelator nicotianamine due to strong constitutive expression of AhNAS2 are important for hyperaccumulation. In order to analyse the relevance of AhNAS2 under more natural conditions representing a range of metalliferous and nonmetalliferous habitats, we collected soil at eight different A. halleri sites and cultivated wild‐type and AhNAS2‐RNAi lines in these soils. AhNAS2 transcript abundance and root nicotianamine concentrations in wild‐type plants were barely influenced by soil metal concentrations. The RNAi effect was fully expressed in different soils. Zn hyperaccumulation in AhNAS2‐silenced lines was significantly reduced in seven soils. Root‐to‐shoot translocation of Cd, Mn, Cu, Ni, and Co was also affected by AhNAS2 silencing, albeit to a lower extent and less consistently. Leaf Fe levels were unaffected by AhNAS2 knockdown. Results demonstrate that elevated nicotianamine production in roots of A. halleri is a Zn hyperaccumulation factor regardless of the edaphic environment, that is, contributes to Zn hyperaccumulation in soils with contrasting Zn availability.

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CAX1 suppresses Cd‐induced generation of reactive oxygen species in Arabidopsis halleri

Cited by 38 publications

References 81 publications

The two copies of the zinc and cadmiumZIP6transporter ofArabidopsis hallerihave distinct effects on cadmium tolerance

The two copies of the zinc and cadmiumZIP6transporter ofArabidopsis hallerihave distinct effects on cadmium tolerance

A Biostimulant Obtained from the Seaweed Ascophyllum nodosum Protects Arabidopsis thaliana from Severe Oxidative Stress

Elevated root nicotianamine concentrations are critical for Zn hyperaccumulation across diverse edaphic environments

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