Cavity enhanced absorption and cavity enhanced magnetic rotation spectroscopy

Engeln, Richard; Berden, Giel; Peeters, Rudy; Meijer, G.C.M.

doi:10.1063/1.1149176

Cited by 322 publications

(189 citation statements)

References 18 publications

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“…The standard deviation in the measurement of the gradient can also be used to calculate the standard deviation in the CEF value. Although for gas-phase cavity measurements the CEF() = and thus can be used to determine the mirror reflectivity at a given wavelength, for the present liquid-phase measurements in a microtiter plate, the CEF value contains significant contributions from both solvent 5 and microtiter plate related scattering and absorption losses and thus is a measure of the total cavity loss.…”

Section: Methodsmentioning

confidence: 99%

“…The results obtained in the studied concentration range were significant as they showed the ability of the system to measure higher analyte concentrations and thus broaden its applicability. [1][2][3][4][5] in the concentration range 0 ng mL -1 to 61.6 ng mL -1 . The average CEF and the standard deviation of the measurement is also listed.…”

Section: Methodsmentioning

confidence: 99%

“…This 'ring down time' could be directly related to the absorption coefficient of an analyte. Later on an experimentally simpler variant, cavity enhanced absorption spectroscopy (CEAS) [5] was demonstrated. This required only the measurement of the time integrated intensity exiting the cavity and so could use slower and cheaper detection setups but had the disadvantage of requiring calibration measurements to obtain the absorption coefficient of analytes.…”

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confidence: 99%

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Cavity-Enhanced Immunoassay Measurements in Microtiter Plates Using BBCEAS

et al. 2016

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We report on the first detailed use of broadband cavity enhanced absorption spectroscopy (BBCEAS) as a detection system for immunoassay. A vertical R ≥ 0.99 optical cavity was integrated with a motorized XY stage, which functioned as a receptacle for 96-well microtiter plates. The custom-built cavity enhanced microplate reader was used to make measurements on a commercially available osteocalcin sandwich ELISA kit. A 30-fold increase in path length was obtained with a minimum detectable change in the absorption coefficient, αmin(t), of 5.3 × 10–5 cm–1 Hz–1/2. This corresponded to a 39-fold increase in the sensitivity of measurement when directly compared to measurements in a conventional microplate reader. Separate measurements of a standard STREP-HRP colorimetric reaction in microtiter plates of differing optical quality produced an increase in sensitivity of up to 115-fold compared to a conventional microplate reader. The sensitivity of the developed setup compared favorably with previous liquid-phase cavity enhanced studies and approaches the sensitivity of typical fluorometric ELISAs. It could benefit any biochemical test which uses single pass absorption as a detection method, through either the label free detection of biologically important molecules at lower concentrations or the reduction in the amount of expensive biochemicals needed for a particular test, leading to cheaper tests.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Cavity-Enhanced Immunoassay Measurements in Microtiter Plates Using BBCEAS

et al. 2016

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“…5,6 The free spectral range of our cavity was 595 MHz and we calculate that the 253.7 nm Hg emission line would excite around 30 longitudinal cavity modes as well as numerous transverse modes. No evidence of mode structure was observed in our experiments.…”

Section: Methodsmentioning

confidence: 99%

“…A more straightforward approach is to directly measure the light intensity transmitted through the cavity, a technique variously known as cavity-enhanced absorption spectroscopy (CEAS) 5 or integrated cavity-output spectroscopy (ICOS). 6,7 Because the cavity mirrors act as an efficient band rejection filter, it is desirable that the light source be extremely bright. Lasers are ideal in this respect, and a wide range of reliable, tuneable and affordable diode lasers is available with output at visible and near-IR wavelengths.…”

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confidence: 99%

Cavity-enhanced absorption using an atomic line source: application to deep-UV measurements

Darby

Smith

Venables

2012

Analyst

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Optical cavities are commonly used to increase the sensitivity of absorption measurements, but have not been extensively used below 300 nm, mainly owing to the limited light sources at these wavelengths. While some progress has been made using cavity ring-down spectroscopy, these systems rely on complex and expensive lasers. Here we investigate an approach combining Cavity-Enhanced Absorption Spectroscopy (CEAS) with an inexpensive low vapour pressure mercury lamp for sensitive absorption measurements at 253.7 nm. We demonstrate that the CEAS absorption in our system is 50 times greater than the absorption found in a single-pass configuration; using this approach, we obtained limits of detection of 8.1 pptv (66 ng m(-3)) for gaseous elemental mercury and 8.4 ppbv for ozone. We evaluate the performance of the system and discuss potential improvements and applications of this approach

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Infrared Cavity Ringdown Spectroscopy

Wagner

Miller

Winstead

2001

Handbook of Vibrational Spectroscopy

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Coherent two‐dimensional vibrational spectroscopies use multiple laser excitations at high intensities where nonlinear processes dominate. These new spectroscopies can potentially probe molecular structure and dynamics in complex systems like multidimensional nuclear magnetic resonance (NMR) methods. We describe the development of the optical analogue to two‐dimensional NMR and its unique capabilities for achieving spectral selectivity. The approach uses four‐wave mixing where three lasers are focused onto a sample and new beams are generated by nonlinear processes. Their intensity depends on whether there are vibrational resonances with the three lasers. We have named these methods doubly vibrationally enhanced four wave mixing (DOVE‐FWM). These methods are related to infrared and Raman spectroscopy, but they are capable of dissecting vibrational spectra into the components that they are made of. These methods offer the potential of a powerful new probe of intra‐ and intermolecular interactions, molecular structure, and molecular dynamics.

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Cavity enhanced absorption and cavity enhanced magnetic rotation spectroscopy

Cited by 322 publications

References 18 publications

Cavity-Enhanced Immunoassay Measurements in Microtiter Plates Using BBCEAS

Cavity-Enhanced Immunoassay Measurements in Microtiter Plates Using BBCEAS

Cavity-enhanced absorption using an atomic line source: application to deep-UV measurements

Infrared Cavity Ringdown Spectroscopy

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