Cavitand‐Grafted Silicon Microcantilevers as a Universal Probe for Illicit and Designer Drugs in Water

Biavardi, Elisa; Federici, Stefania; Tudisco, Cristina; Menozzi, Daniela; Massera, Chiara; Sottini, Andrea; Condorelli, Guglielmo G.; Bergese, Paolo; Dalcanale, Enrico

doi:10.1002/anie.201404774

Cited by 52 publications

(28 citation statements)

References 37 publications

(29 reference statements)

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“…The surface stress generated by the interaction gives (in modulus) Δσ = 12 mN m −1 for MDMA and cocaine and Δσ = 5 mN m −1 for amphetamine, in fully agreement with several previously reported studies, all related to biomolecules, where it is reported that recognition triggers 1 mN m −1 <Δσ< 50 mN m −1 [26,[54][55][56]. Other common excipients used in drug formulation, such as lactose and glucose, were investigated with the cavitand-MC system, and both MDMA and cocaine are recognized with high fidelity in the presence of the excipients [53].…”

Section: Illicit and Designer Drugssupporting

confidence: 90%

“…Actually, designer variants, featuring minor modifications with respect to an existing drug, show a different chemical composition and are currently not illegal in many jurisdictions. The cavitand-MC system overcomes this shortcoming for methamphetamines, being capable to recognize the methyl-amine portion that is common to the entire drug family [53].…”

Section: Illicit and Designer Drugsmentioning

confidence: 99%

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Surface Nanomechanics of Biomolecules and Supramolecular Systems

Bergese¹,

Federici²

2017

Nanomechanics

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Surface nanomechanics of biomolecules and supramolecular systems is an interdisciplinary and vital area of current research, with implications/applications spanning from synthetic biology to regenerative medicine, from smart surfaces to molecular machines. Biomolecule surface transformations and nanomachinery arise upon "wiring" them onto surfaces and interfaces. Surface confinement of biomolecules is a common feature of biological systems (e.g., cell membranes) and often a mandatory step for translating their properties into real-world applications (e.g., biosensors). On surfaces biomolecules undergo peculiar transformations and interactions which they do not experience in solution. Such unedited effects open challenges in synthetic systems, for example, by altering or hindering the designed/expected property, but also disclose a wealth of opportunities and surprises. Based on our latest research, this chapter will bring fresh excerpts from the field. It will start with an accessible description of thermodynamics of surface nanomechanics of biomolecules and supramolecular systems and then will show how it can be implemented to gain understanding of grow factor cell signaling, to single out small ligands able to inhibit protein misfolding, to measure energetics of surface confined ferritin during iron loading, and to realize a universal probe for ammine-based designer drugs.

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Section: Illicit and Designer Drugssupporting

confidence: 90%

Section: Illicit and Designer Drugsmentioning

confidence: 99%

Surface Nanomechanics of Biomolecules and Supramolecular Systems

Bergese¹,

Federici²

2017

Nanomechanics

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“…A nanomechanical device with a detection limit of 10 ¡4 M for MDMA has been recently reported. [32] The limit of detection by GC-FTIR for amphetamin-type stimulants has been shown to range from a few ng to about 100À200 ng. [33] The presence of micromolar concentration of MDMA in saliva has been measured by GC-MS. [35] In fact, our strategy is simple and does not require sophisticated instruments.…”

Section: Drug Assaymentioning

confidence: 99%

Unmodified gold nanoparticles as a colorimetric probe for visual methamphetamine detection

Yarbakht

Nikkhah

2015

Journal of Experimental Nanoscience

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In recent years, in addition to the classic drugs, addiction to a series of new drug classes known as club drugs has increased significantly. Fast and lowcost bioassay for the detection of amphetamine-based drugs can be an effective strategy towards reducing their abuse. In this study, we designed a sensitive bioassay strategy using gold nanoparticles (GNPs) and the aptamers that possess high affinity toward methamphetamine (MA). It is suggested that the aptamer adopts different tertiary structures in the presence and/or absence of its specific target and GNPs can effectively differentiate between these two states by their characteristic surface plasmon resonance-based colour change. Visual detection of MA and 3,4-methylenedioxy-N-methylamphetamine (MDMA) in the low micromolar range is possible within minutes with the use of this method.

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“…tensiometric biosensors to label-free study of molecular collective surface nanomechanics [33][34][35][36], opening new insights in surfaces confined molecular transformations and machines [32,[37][38][39][40], up to single cell investigation [41][42][43]. Experiments exploiting static deflection of MC beams are reported in literature to study the chemi-or physisorption of synthetic lipid bilayer on a cantilever surface, on either gold (Au) or silicon dioxide (SiO 2 ) surfaces [17,18].…”

Section: Interaction Between Extracellular Vesicles and Microcantileversmentioning

confidence: 99%

Interaction of Extracellular Vesicles with Si Surface Studied by Nanomechanical Microcantilever Sensors

et al. 2018

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Abstract:We report on the interaction of small (<150 nm) extracellular vesicles (EVs) with silicon surface. The study is conducted by leveraging Si nanomechanical microcantilever sensors actuated in static and dynamic modes, that allow tracking of EV collective adsorption energy and adsorbed mass. Upon incubation for 30 min at about 10 nM concentration, EVs isolated from human vascular endothelial cell (HVEC) lines form a patchy layer that partially covers the Si total surface. Formation of this layer releases a surface energy equal to (8 ± 1) mJ/m 2 , typical of weak electrostatic interactions. These findings give a first insight into the EV-Si interface and proof the possibility to realize new hybrid biointerphases that can be exploited as advanced models to investigate properties of biological membranes and/or biosensing platforms that take advantage of biomolecules embedded/supported in membranes.

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Cavitand‐Grafted Silicon Microcantilevers as a Universal Probe for Illicit and Designer Drugs in Water

Cited by 52 publications

References 37 publications

Surface Nanomechanics of Biomolecules and Supramolecular Systems

Surface Nanomechanics of Biomolecules and Supramolecular Systems

Unmodified gold nanoparticles as a colorimetric probe for visual methamphetamine detection

Interaction of Extracellular Vesicles with Si Surface Studied by Nanomechanical Microcantilever Sensors

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