Caveolin-1 Expression and Caveolae Biogenesis during Cell Transdifferentiation in Lung Alveolar Epithelial Primary Cultures

Campbell, Lee Ann; Hollins, Andrew John; Aleid, Adham; Newman, Geoffrey R.; Ruhland, Christopher John Von; Gumbleton, Mark

doi:10.1006/bbrc.1999.1280

Cited by 118 publications

(104 citation statements)

References 47 publications

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“…This observation, which would indicate that type II cells lack caveolae and therefore contain rafts, is consistent with electron micrographic studies demonstrating that type II cells are devoid of caveolae or immunogold-detectable caveolin (30,178).…”

Section: Lpp In Signaling Platformssupporting

confidence: 89%

“…This process is inhibited by NEM and cholesterol-binding agents such as filipin and appears to require GTP (4). Transmembrane transport has been studied extensively in endothelial cells but appears to function in placenta epithelial cells as well and may occur in alveolar type I cells (30,178).…”

Section: Lpp In Signaling Platformsmentioning

confidence: 99%

“…It is well recognized that type II cells in culture lose their characteristic cuboidal morphology, lamellar bodies, and surfactant protein expression and acquire a more type I cell phenotype (30,54,55,226 (30,176,178). Interestingly, trans-/dedifferentiation of type II cells on tissue culture plastic is also accompanied by a fourfold increase in LPP activity.…”

Section: Lpp In Signaling Platformsmentioning

confidence: 99%

“…During recovery from injury, these cells proliferate, migrate, and transdifferentiate into type I cells to re-establish the lung epithelial barriers (1,28,29,30). Cyclin cdc2 expression is upregulated after hyperoxidative stress (275), but whether reentry of G 0 type II cells to G 1 phase during other forms of lung injury involves cyclin-dependent phosphorylation and whether expression of these activities diminishes during transdifferentiation to type I cells must still be determined.…”

Section: Pap1mentioning

confidence: 99%

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Pulmonary phosphatidic acid phosphatase and lipid phosphate phosphohydrolase

Nanjundan

Possmayer

2003

American Journal of Physiology-Lung Cellular and Molecular Phys

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The lung contains two distinct forms of phosphatidic acid phosphatase (PAP). PAP1 is a cytosolic enzyme that is activated through fatty acid-induced translocation to the endoplasmic reticulum, where it converts phosphatidic acid (PA) to diacylglycerol (DAG) for the biosynthesis of phospholipids and neutral lipids. PAP1 is Mg2+ dependent and sulfhydryl reagent sensitive. PAP2 is a six-transmembrane-domain integral protein localized to the plasma membrane. Because PAP2 degrades sphingosine-1-phosphate (S1P) and ceramide-1-phosphate in addition to PA and lyso-PA, it has been renamed lipid phosphate phosphohydrolase (LPP). LPP is Mg2+independent and sulfhydryl reagent insensitive. This review describes LPP isoforms found in the lung and their location in signaling platforms (rafts/caveolae). Pulmonary LPPs likely function in the phospholipase D pathway, thereby controlling surfactant secretion. Through lowering the levels of lyso-PA and S1P, which serve as agonists for endothelial differentiation gene receptors, LPPs regulate cell division, differentiation, apoptosis, and mobility. LPP activity could also influence transdifferentiation of alveolar type II to type I cells. It is considered likely that these lipid phosphohydrolases have critical roles in lung morphogenesis and in acute lung injury and repair.

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Section: Lpp In Signaling Platformssupporting

confidence: 89%

Section: Lpp In Signaling Platformsmentioning

confidence: 99%

Section: Lpp In Signaling Platformsmentioning

confidence: 99%

Section: Pap1mentioning

confidence: 99%

See 2 more Smart Citations

Pulmonary phosphatidic acid phosphatase and lipid phosphate phosphohydrolase

Nanjundan

Possmayer

2003

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…Most interesting, in primary culture, alveolar type II cells growing on a plastic substratum acquire an alveolar type I phenotype as evidenced by an increase in many type I cell-specific proteins including caveolin-1. In these cells, caveolin-1 expression coincides with the formation of caveolae and a down-regulation of SP-C (20,21). To date, there are no studies reporting the identification of ciselements or transcription factors important for caveolin-1 transcription in lung epithelial and endothelial cells.…”

mentioning

confidence: 99%

Transcription of the Caveolin-1 Gene Is Differentially Regulated in Lung Type I Epithelial and Endothelial Cell Lines

Kathuria¹,

Cao²,

Ramirez³

et al. 2004

Journal of Biological Chemistry

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In the lung, caveolin-1 is expressed in both type I alveolar epithelial and endothelial cells where it is hypothesized to modulate molecular signaling activities and progression of tumorigenesis. Developmentally, caveolin-1␣ is expressed in fetal lung endothelial, but not epithelial, cells; in adult lung, both cell types express caveolin-1␣. To test the hypothesis that caveolin-1 transcription is differentially regulated in type I and endothelial cells, we characterized the proximal promoter of the mouse caveolin-1 gene in lung cell lines to identify factors that control its cell-specific expression. We show that caveolin-1 expression is regulated by an Ets ciselement in a lung epithelial cell line, but not a lung endothelial cell line, and that three ETS family members, ETS-1, PEA3, and ERM, recognize and bind the Ets site in the epithelial cell line. Based on these findings, we have identified the Ets cis-element as a region that accounts for differential transcriptional regulation of caveolin-1 in lung epithelial and endothelial cells.The caveolin-1 promoter of several species has been cloned and sequenced, yet little is known about the protein transcription factors and cis-elements that regulate its transcription. In NIH 3T3 cells, caveolin promoter constructs containing 750 bp or 3 kb of upstream sequence show similar promoter activity (1), suggesting that in this cell line most of the regulatory regions are contained within the first 750 bp of the caveolin-1 promoter. In normal human skin fibroblasts, the caveolin-1 promoter is regulated by Sp1, p53, E2F/DP-1, and serum-response element-specific enhancers (2), whereas in vascular smooth muscle cells increases in free cholesterol stimulate caveolin-1 transcription by an SREBP-1-dependent mechanism (3).The regulation of expression of caveolin-1 in the lung is especially interesting both because it is developmentally regulated and because two extensive cell populations expressing high levels of caveolin-1, i.e. alveolar epithelial type I cells and alveolar capillary endothelial cells, reside within a short distance from each other and therefore share many environmental influences (4). Furthermore, targeted deletions of caveolin-1 in mice show mainly a pulmonary phenotype, pulmonary hypertension as well as hyperproliferative and fibrotic lung tissue (5-7). Consistent with this hyperproliferative phenotype, embryonic fibroblasts derived from the null animals appear to have an increased rate of proliferation, which is partially reversed by re-expressing caveolin-1 protein from a viral expression vector (5). In intact animals loss of caveolin-1 leads to lung abnormalities at 2-4 months, about the same postnatal time point that caveolin-1␣ is first detectable by immunohistochemistry in type I cells in normal animals (4). The pulmonary phenotype appears to contribute to the shortened life span of the null animals although there are uncertainties about the actual cause of early death (8).Caveolin-1, the main structural protein of caveolae, is a 21-24-kDa integral me...

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Isolation and Culture of Pulmonary Alveolar Epithelial Type II Cells

Dobbs¹,

Gonzalez²

2002

Culture of Epithelial Cells

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Caveolin-1 Expression and Caveolae Biogenesis during Cell Transdifferentiation in Lung Alveolar Epithelial Primary Cultures

Cited by 118 publications

References 47 publications

Pulmonary phosphatidic acid phosphatase and lipid phosphate phosphohydrolase

Pulmonary phosphatidic acid phosphatase and lipid phosphate phosphohydrolase

Transcription of the Caveolin-1 Gene Is Differentially Regulated in Lung Type I Epithelial and Endothelial Cell Lines

Isolation and Culture of Pulmonary Alveolar Epithelial Type II Cells

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