Cav1.2 regulated odontogenic differentiation of NG2+ pericytes during pulp injury

Fu, Yunyu; Ju, Yanqin; Zhao, Shouliang

doi:10.1007/s10266-022-00720-w

Cited by 4 publications

(5 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, our results showed that hPSC-derived pericytes markedly enhanced the in vitro tube formation ability of HUVECs. Previous studies also showed that NG2 + pericytes in dental pulp have the ability to differentiate into odontoblasts [13]. Moreover, through bulk RNA-seq and single cell RNA-seq analysis, Val Yianni and Paul T. Sharpe [31] discovered that mouse pericytes have an immunomodulatory role as they express multiple cytokines and chemokines within the dental pulp.…”

Section: Discussionmentioning

confidence: 99%

“…A set of markers has commonly been used to define pericytes, including Pdgfrβ, Desmin, NG2, RGS5, 3G5, CD13, and αSMA [36,37]. The lineage tracing of dental pulp pericytes has been successfully achieved using several transgenic mice models, such as NG2-CreERT2, Fabp4-lacZ, and Tagln-Cre [13,31]. Here, our results showed that, similar to CD13 and NG2, Pdgfrβ could be used as a suitable marker for the labeling of pericytes in dental pulp.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies showed that dental pulp pericytes possess odontogenic differentiation abilities and immunoregulatory abilities [13,31]. To detect the multilineage differentiation potential of hPSC-derived pericytes, CNC PCs were cultured in osteogenic, adipogenic, and chondrogenic induction media for 2-4 weeks.…”

Section: Hpsc-derived Pericytes Have the Ability To Undergo Multiline...mentioning

confidence: 99%

“…When pulpitis occurs, sensory nerve fibers are activated by tissue hypoxia and pain and then secrete neuropeptides, which in turn lead to vasodilation and increased vascular permeability, and eventually result in pericyte death, vascular wall rupture, and other phenotypes [9,11]. It has been reported that endogenous dental pulp pericytes possess immunomodulatory properties [12] and can differentiate into odontoblasts and promote the repair of dental pulp injury in vivo [13]. These results strongly indicated the essential role of pericytes in dental pulp homeostasis, which prompt us to hypothesize that the restoration of pericytes may help to relieve the inflammation index and may represent a potential therapeutic option during pulpitis.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Efficient Treatment of Pulpitis via Transplantation of Human Pluripotent Stem Cell-Derived Pericytes Partially through LTBP1-Mediated T Cell Suppression

Li,

Chiu

et al. 2023

Biomedicines

View full text Add to dashboard Cite

Dental pulp pericytes are reported to have the capacity to generate odontoblasts and express multiple cytokines and chemokines that regulate the local immune microenvironment, thus participating in the repair of dental pulp injury in vivo. However, it has not yet been reported whether the transplantation of exogenous pericytes can effectively treat pulpitis, and the underlying molecular mechanism remains unknown. In this study, using a lineage-tracing mouse model, we showed that most dental pulp pericytes are derived from cranial neural crest. Then, we demonstrated that the ablation of pericytes could induce a pulpitis-like phenotype in uninfected dental pulp in mice, and we showed that the significant loss of pericytes occurs during pupal inflammation, implying that the transplantation of pericytes may help to restore dental pulp homeostasis during pulpitis. Subsequently, we successfully generated pericytes with immunomodulatory activity from human pluripotent stem cells through the intermediate stage of the cranial neural crest with a high level of efficiency. Most strikingly, for the first time we showed that, compared with the untreated pulpitis group, the transplantation of hPSC-derived pericytes could substantially inhibit vascular permeability (the extravascular deposition of fibrinogen, ** p < 0.01), alleviate pulpal inflammation (TCR+ cell infiltration, * p < 0.05), and promote the regeneration of dentin (** p < 0.01) in the mouse model of pulpitis. In addition, we discovered that the knockdown of latent transforming growth factor beta binding protein 1 (LTBP1) remarkably suppressed the immunoregulation ability of pericytes in vitro and compromised their in vivo regenerative potential in pulpitis. These results indicate that the transplantation of pericytes could efficiently rescue the aberrant phenotype of pulpal inflammation, which may be partially due to LTBP1-mediated T cell suppression.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Hpsc-derived Pericytes Have the Ability To Undergo Multiline...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Efficient Treatment of Pulpitis via Transplantation of Human Pluripotent Stem Cell-Derived Pericytes Partially through LTBP1-Mediated T Cell Suppression

Li,

Chiu

et al. 2023

Biomedicines

View full text Add to dashboard Cite

show abstract

“…DPSCs transfected with hypoxia-responsive elements and FGF2 differentiated into CD13 + pericytes and secreted FGF2 to improve the expression of N-cadherin and promote the adhesion of CD13 + pericytes to endothelial cells ( Zhu S. et al, 2021 ). NG2 + hDPCs sorted from hDPCs were cultured in human pericyte medium and differentiated into NG2 + pericytes ( Fu et al, 2023 ). In addition, FGF2 in endothelial cell growth medium induced DPSCs to differentiate into NG2 + pericytes and further supported the proliferation and migration of pericytes ( Delle Monache et al, 2019 ).…”

Section: Potential Cell Sources Of Prevascularization Techniquesmentioning

confidence: 99%

Prevascularization techniques for dental pulp regeneration: potential cell sources, intercellular communication and construction strategies

Qiao

Tan

Guo

et al. 2023

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

One of the difficulties of pulp regeneration is the rapid vascularization of transplanted engineered tissue, which is crucial for the initial survival of the graft and subsequent pulp regeneration. At present, prevascularization techniques, as emerging techniques in the field of pulp regeneration, has been proposed to solve this challenge and have broad application prospects. In these techniques, endothelial cells and pericytes are cocultured to induce intercellular communication, and the cell coculture is then introduced into the customized artificial vascular bed or induced to self-assembly to simulate the interaction between cells and extracellular matrix, which would result in construction of a prevascularization system, preformation of a functional capillary network, and rapid reconstruction of a sufficient blood supply in engineered tissue after transplantation. However, prevascularization techniques for pulp regeneration remain in their infancy, and there remain unresolved problems regarding cell sources, intercellular communication and the construction of prevascularization systems. This review focuses on the recent advances in the application of prevascularization techniques for pulp regeneration, considers dental stem cells as a potential cell source of endothelial cells and pericytes, discusses strategies for their directional differentiation, sketches the mechanism of intercellular communication and the potential application of communication mediators, and summarizes construction strategies for prevascularized systems. We also provide novel ideas for the extensive application and follow-up development of prevascularization techniques for dental pulp regeneration.

show abstract

Use of Neuroglial Antigen 2 (NG2)-expressing Mesenchymal Stem-Like Cells for Liver Fibrosis/Cirrhosis: A New Scene

Zhang,

Lin,

Yang

et al. 2023

Handbook of Stem Cell Applications

View full text Add to dashboard Cite

Cav1.2 regulated odontogenic differentiation of NG2+ pericytes during pulp injury

Cited by 4 publications

References 41 publications

Efficient Treatment of Pulpitis via Transplantation of Human Pluripotent Stem Cell-Derived Pericytes Partially through LTBP1-Mediated T Cell Suppression

Efficient Treatment of Pulpitis via Transplantation of Human Pluripotent Stem Cell-Derived Pericytes Partially through LTBP1-Mediated T Cell Suppression

Prevascularization techniques for dental pulp regeneration: potential cell sources, intercellular communication and construction strategies

Use of Neuroglial Antigen 2 (NG2)-expressing Mesenchymal Stem-Like Cells for Liver Fibrosis/Cirrhosis: A New Scene

Contact Info

Product

Resources

About