Cav1.2 of L-type Calcium Channel Is a Key Factor for the Differentiation of Dental Pulp Stem Cells

Ju, Yanqin; Ge, Jianping; Ren, Xudong; Zhu, Xianmin; Xue, Zhigang; Feng, Yun; Zhao, Shouliang

doi:10.1016/j.joen.2015.01.009

Cited by 27 publications

(29 citation statements)

References 35 publications

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“…Regarding the electrical nature of DPSCs, undifferentiated stem cells are thought to be non‐excitable. However, DPSCs have VDCCs of the Cav 1.2 type, which is the main pathway for Ca 2+ influx in excitable cells . This is in line with our findings in undifferentiated human adipose‐derived stem cells that express the Cav 1.2.…”

Section: Discussionsupporting

confidence: 91%

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Transient receptor potential melastatin 4 channel is required for rat dental pulp stem cell proliferation and survival

et al. 2017

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Section: Discussionsupporting

confidence: 91%

“…The presence of VDCCs in rat DPSCs was reported by Ju et al. and is necessary for neuron and osteoblast differentiation . Another family member, TRPM7, is a Ca 2+ ‐ and Mg 2+ ‐permeable channel that is essential for cell proliferation and survival .…”

Section: Introductionmentioning

confidence: 79%

Transient receptor potential melastatin 4 channel is required for rat dental pulp stem cell proliferation and survival

et al. 2017

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“…Ca v 1.2 is widely expressed in various tissues and regulates multiple cell functions. In term of cell differentiation, it participates in the process of osteogenic (Wen et al, ), odontogenic (Ju et al, ), and neural differentiation (Obermair, Szabo, Bourinet, & Flucher, ). Abnormity of Ca v 1.2 can lead to several disorders (Zamponi et al, ), including Timothy syndrome, cardiac arrhythmias, and neuropsychiatric diseases.…”

Section: Discussionmentioning

confidence: 99%

Ca_v1.2 regulates osteogenesis of bone marrow‐derived mesenchymal stem cells via canonical Wnt pathway in age‐related osteoporosis

Fei

Zhang

et al. 2019

Aging Cell

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Aims Age‐related bone mass loss is one of the most prevalent diseases that afflict the elderly population. The decline in the osteogenic differentiation capacity of bone marrow‐derived mesenchymal stem cells (BMMSCs) is regarded as one of the central mediators. Voltage‐gated Ca2+ channels (VGCCs) play an important role in the regulation of various cell biological functions, and disruption of VGCCs is associated with several age‐related cellular characteristics and systemic symptoms. However, whether and how VGCCs cause the decreased osteogenic differentiation abilities of BMMSCs have not been fully elucidated. Methods Voltage‐gated Ca2+ channels related genes were screened, and the candidate gene was determined in several aging models. Functional role of determined channel on osteogenic differentiation of BMMSCs was investigated through gain and loss of function experiments. Molecular mechanism was explored, and intervention experiments in vivo and in vitro were performed. Results We found that Cav1.2 was downregulated in these aging models, and downregulation of Cav1.2 in Zmpste24−/− BMMSCs contributed to compromised osteogenic capacity. Mechanistically, Cav1.2 regulated the osteogenesis of BMMSCs through canonical Wnt/β‐catenin pathway. Moreover, upregulating the activity of Cav1.2 mitigated osteoporosis symptom in Zmpste24−/− mice. Conclusion Impaired osteogenic differentiation of Zmpste24−/− BMMSCs can be partly attributed to the decreased Cav1.2 expression, which leads to the inhibition of canonical Wnt pathway. Bay K8644 treatment could be an applicable approach for treating age‐related bone loss by ameliorating compromised osteogenic differentiation capacity through targeting Cav1.2 channel.

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“…In view of our previous studies on calcium channels (Ju et al, 2015; Gao et al, 2017), we focused on the role of the TRPC channel family, including the candidate regulatory genes, TRPC1 and TRPC3 . In addition, the GO analysis showed that tooth development is closely related to single-organism cellular processes, intracellular component, binding and protein binding functions (Appendix Figure 2A).…”

Section: Resultsmentioning

confidence: 99%

RNA-Sequencing Analyses Demonstrate the Involvement of Canonical Transient Receptor Potential Channels in Rat Tooth Germ Development

Yang

Cai

et al. 2017

Front. Physiol.

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Tooth development depends on multiple molecular interactions between the dental epithelium and mesenchyme, which are derived from ectodermal and ectomesenchymal cells, respectively. We report on a systematic RNA sequencing analysis of transcriptional expression levels from the bud to hard tissue formation stages of rat tooth germ development. We found that GNAO1, ENO1, EFNB1, CALM1, SIAH2, ATP6V0A1, KDELR2, GTPBP1, POLR2C, SORT1, and members of the canonical transient receptor potential (TRPC) channel family are involved in tooth germ development. Furthermore, Cell Counting Kit 8 (CCK8) and Transwell migration assays were performed to explore the effects of these differentially expressed genes (DEGs) on the proliferation and migration of dental pulp stem cells. Immunostaining revealed that TRPC channels are expressed at varying levels during odontogenesis. The identified genes represent novel candidates that are likely to be vital for rat tooth germ development. Together, the results provide a valuable resource to elucidate the gene regulatory mechanisms underlying mammalian tooth germ development.

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Cav1.2 of L-type Calcium Channel Is a Key Factor for the Differentiation of Dental Pulp Stem Cells

Cited by 27 publications

References 35 publications

Transient receptor potential melastatin 4 channel is required for rat dental pulp stem cell proliferation and survival

Transient receptor potential melastatin 4 channel is required for rat dental pulp stem cell proliferation and survival

Ca_v1.2 regulates osteogenesis of bone marrow‐derived mesenchymal stem cells via canonical Wnt pathway in age‐related osteoporosis

RNA-Sequencing Analyses Demonstrate the Involvement of Canonical Transient Receptor Potential Channels in Rat Tooth Germ Development

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Cav1.2 of L-type Calcium Channel Is a Key Factor for the Differentiation of Dental Pulp Stem Cells

Cited by 27 publications

References 35 publications

Transient receptor potential melastatin 4 channel is required for rat dental pulp stem cell proliferation and survival

Transient receptor potential melastatin 4 channel is required for rat dental pulp stem cell proliferation and survival

Cav1.2 regulates osteogenesis of bone marrow‐derived mesenchymal stem cells via canonical Wnt pathway in age‐related osteoporosis

RNA-Sequencing Analyses Demonstrate the Involvement of Canonical Transient Receptor Potential Channels in Rat Tooth Germ Development

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Ca_v1.2 regulates osteogenesis of bone marrow‐derived mesenchymal stem cells via canonical Wnt pathway in age‐related osteoporosis