Causes of aneuploidy – polar body-based PGD

Magli, M.C.; Gianaroli, Luca; Crippa, Andor; Grugnetti, C.; Ruberti, Alessandra; Ferraretti, AP

doi:10.1016/s1472-6483(10)61200-x

Cited by 2 publications

(3 citation statements)

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“…A non-random distribution of missing and extra chromatids and chromosomes (2:1 ratio, respectively; Figure 2) is also in agreement with Magli et al (2009), suggesting that this might be an important biological mechanism, preventing the extrusion of extra chromosome material into the PB1 in the event of meiosis I errors. It has previously been demonstrated that there is an age dependence of both missing chromatids and missing chromosomes, increasing from 45% to 70% for missing chromatids, and from 4% to 8% for missing chromosomes, between the ages of 35 and 43 years .…”

Section: Inconsistency Between Aneuploidy Types Predicted By Pb1 and supporting

confidence: 57%

Meiosis errors in over 20,000 oocytes studied in the practice of preimplantation aneuploidy testing

Kuliev

Zlatopolsky

Kirillova

et al. 2011

Reproductive BioMedicine Online

160

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This study presents the world’s largest series of over 20,000 oocytes tested for aneuploidies, involving chromosomes 13,16, 18, 21 and 22, providing the data on the rates and types of aneuploidies and their origin. Almost every second oocyte (46.8%) is abnormal, with predominance of extra chromatid errors predicting predominance of trisomies (53%) over monosomies (26%) in the resulting embryos (2:1), which is opposite to monosomy predominance observed in embryo testing. Of the detected anomalies in oocytes, 40% are complex, so testing for a few most prevalent chromosome errors may allow detection of the majority of abnormal embryos. Chromosome 21 and 22 errors are more prevalent, while two different patterns of error origin were observed for different chromosomes: chromosome 16 and 22 errors originate predominantly from meiosis II, compared with chromosome 13, 18 and 21 errors originating from meiosis I. This provides the first evidence for the differences in the aneuploid embryo survival depending on the meiotic origin. Considering the problem of mosaicism, which is the major limitation of the cleavage-stage testing, the direct oocyte aneuploidy testing by polar body analysis may be of obvious practical value in improving accuracy and reliability of avoiding aneuploid embryos for transfer.

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Section: Inconsistency Between Aneuploidy Types Predicted By Pb1 and supporting

confidence: 57%

Meiosis errors in over 20,000 oocytes studied in the practice of preimplantation aneuploidy testing

Kuliev

Zlatopolsky

Kirillova

et al. 2011

Reproductive BioMedicine Online

160

View full text Add to dashboard Cite

show abstract

“…The abnormalities are represented by different types of errors, involving more than one chromosome, or errors in both MI and MII of the same or different chromosomes that may result in balanced (normal) embryos. This is in agreement with other reported data [29,42] may represent the phenomenon of aneuploidy rescue, similar to the well-known trisomy rescue mechanism. The resulting balanced chromosome set of the oocyte after complementary errors in meiosis I and meiosis II is shown in Fig.…”

Section: Meiotic and Mitotic Abnormalitiessupporting

confidence: 94%

“…6.1). This is in agreement with a study of 684 cycles from infertility patients that showed 55% aneuploidy rate deriving from meiosis errors [28,29]. The meiosis I errors were 39% in the younger than 38-year-old patients and 58% in 44-year-old patients [30].…”

Section: Meiotic and Mitotic Abnormalitiessupporting

confidence: 92%