Quality is one of an important parameter for measuring successful and sustainable construction projects. For a successful construction projects, it is essential that the projects are delivered according to the quality standards and in line with the customer desire. However, construction projects in both developed and developing countries are characterized by poor quality and highway projects in Pakistan has no exception. This paper aims to determine factors adversely affecting quality in highway projects of Pakistan. Research method for this study is a combination of both literature review and questionnaire survey. Literature review enabled identification of 24 common factors adversely affecting quality in construction industry. A questionnaire based on literature review was distributed amongst construction professionals of highway projects in Pakistan. Data collected from 215 respondents was analyzed using Statistical Package for the Social Sciences (SPSS). It was discovered that incompetent main contractors, improper planning, and improper selection of materials are the main factors adversely affecting quality in highway projects of Pakistan. The findings of this study can assist construction professionals to develop strategies to improve the quality of construction projects in general and highway projects particularly.