Cationic Surfactant-Induced Instantaneous Gelation of Silk Fibroin Solution

Xing, Tieling; Li, Jiaojiao; Sun, Shan; Jin, Jian; Zhang, Fang; Lu, Shenzhou

doi:10.14233/ajchem.2014.18180

Cited by 5 publications

(4 citation statements)

References 16 publications

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“…The addition of SF to the Tween 80-stabilized emulsion also improved the stability of oil droplets, but high-speed centrifugation destroyed the droplet structure, leading to the agglomeration of oil droplets and the formation of SF aggregates (Figure g,h). This change was likely caused by a structural change with the SF and gelation induced by Tween 80, a phenomenon previously described . Furthermore, when the size distribution was examined with the SF-added nSiO 2 -stabilized Pickering emulsion before and after centrifugation, there was no significant difference (∼6 μm, p > 0.05) in the size of oil droplets (inset in Figure c,d,g,h), confirming the conclusion that the combination of SF/nSiO 2 significantly improved the stability of the oil droplets, likely by forming stable shells at the oil/water interface.…”

Section: Resultssupporting

confidence: 79%

“…This change was likely caused by a structural change with the SF and gelation induced by Tween 80, a phenomenon previously described. 50 Furthermore, when the size distribution was examined with the SF-added nSiO 2stabilized Pickering emulsion before and after centrifugation, there was no significant difference (∼6 μm, p > 0.05) in the size of oil droplets (inset in Figure 1c,d,g,h), confirming the conclusion that the combination of SF/nSiO 2 significantly improved the stability of the oil droplets, likely by forming stable shells at the oil/water interface. It has been known that the coexistence of alternating hydrophobic and hydrophilic domains endows SF with amphiphilic capacity, and stable oil/ SF emulsions have been prepared based on SF 51 and SF nanofibers, 33 which self-assembled at the oil/water interface and lowered surface tension.…”

Section: Resultsmentioning

confidence: 52%

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Tuning Microcapsule Shell Thickness and Structure with Silk Fibroin and Nanoparticles for Sustained Release

Wang

Cheng

Liu

et al. 2020

ACS Biomater. Sci. Eng.

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Microcapsules have attracted widespread interest for their unique properties in encapsulation, protection, and separation of active ingredients from the surrounding environment. However, microcapsule carriers with controllable shell thickness, permeability, good mechanical properties, and thermostability are challenging to obtain. Herein, robust and versatile composite microcapsules were fabricated using SiO 2 nanoparticle-stabilized (Pickering) oil emulsions as core templates, while silk fibroin (SF) was assembled at the oil/ water interface. This process resulted in the formation of physically and chemically stable microcapsules with a thick (∼800 nm) shell that protected the encapsulated ingredient from high shear forces and high temperatures during spray-drying. SiO 2 nanoparticles were randomly distributed in the shell matrix after preparation, making the microcapsules mechanically robust (4.48 times higher than control samples prepared using surfactant Tween 80 instead of the SiO 2 nanoparticles), as well as thermostable (retained shape to 900 °C). The microcapsules displayed tunable drug release by adjusting the SF content in the shell. Under optimal conditions (weight ratio of SiO 2 /SF = 7:10, corn oil content about 55 wt %), a model drug (curcumin) was encapsulated in the SF microcapsules with an encapsulation efficiency up to 95%. The in vitro drug release from these SF microcapsules lasted longer than control microcapsules, demonstrating the capability of these novel microcapsules in sustaining drug release.

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Section: Resultssupporting

confidence: 79%

Section: Resultsmentioning

confidence: 52%

Tuning Microcapsule Shell Thickness and Structure with Silk Fibroin and Nanoparticles for Sustained Release

Wang

Cheng

Liu

et al. 2020

ACS Biomater. Sci. Eng.

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“…Recent studies revealed that the slow dynamics of gelation for a pure SF solution is mainly relevant with conformational transition from random coil to β-sheet structure, which then gradually self-assembles into close-packed β-sheet crystals, acting as a physical cross-link to stabilize SF hydrogel. ,, Although many physical treatment methods (e.g., pH, vortexing, sonication, and electrical field), − or the addition of certain organic molecules (e.g., ethanol, surfactants, or hydrophilic polymers), are able to increase the gelation rate of SF by adjusting protein chain–chain interactions, − the gelation process induced by instrumental parameters may not be compatible with certain clinical environments, and potential toxicity of certain organic molecules can raise a significant concern for biomedical applications. Thus, searching for a simple and biocompatible way to trigger the gelation of SF is highly desirable.…”

Section: Introductionmentioning

confidence: 99%

Cooperative Assembly of a Peptide Gelator and Silk Fibroin Afford an Injectable Hydrogel for Tissue Engineering

Cheng

Yan

et al. 2018

ACS Appl. Mater. Interfaces

102

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Silk fibroin (SF) from Bombyx mori has received increasing interest in biomedical fields, because of its slow biodegradability, good biocompatibility, and low immunogenicity. Although SF-based hydrogels have been studied intensively as a potential matrix for tissue engineering, weak gelation performance and low mechanical strength are major limitations that hamper their widespread applicability. Therefore, searching for new strategies to improve the SF gelation property is highly desirable in tissue engineering research. Herein, we report a facile approach to induce rapid gelation of SF by a small peptide gelator (e.g., NapFF). Following the simple mixing of SF and NapFF in water, a stable hydrogel of SF was obtained in a short time period at physiological pH, and the minimum gelation concentration of SF can reach as low as 0.1%. In this process of gelation, NapFF not only can behave itself as a gelator for supramolecular self-assembly, but also can trigger the conformational transition of the SF molecule from random coil to β-sheet structure via hydrophobic and hydrogen-bonding interactions. More importantly, for the generation of a scaffold with favorable cell-surface interactions, a new peptide gelator (NapFFRGD) with Arg-Gly-Asp (RGD) domain was applied to functionalize SF hydrogel with improved bioactivity for cell adhesion and growth. Following encapsulating the vascular endothelial growth factor (VEGF), the SF gel was subcutaneously injected in mice, and served as an effective matrix to trigger the generation of new blood capillaries in vivo.

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“…Besides the role of negatively charged surfactants in inducing SF gelation, the effects of non-ionic and cationic surfactants were also evaluated [100,101]. The chemical structures of the surfactants were illustrated in Figure 11.…”

Section: Figure 10 Chemical Structure Of Sos Sds and Stsmentioning

confidence: 99%

Phospholipid- and gold-induced gelation of Thai silk fibroin and its applications for cell and drug delivery

Laomeephol

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Recently, an�in situ�gelation, which is a rapid sol-to-gel transition under mild conditions, has been employed, allowing an entrapment of cells or substances in 3D structures as well as tailorable geometries and point-of-care uses. Silk fibroin (SF), a natural polymer from�Bombyx mori�silkworm cocoons, was chosen in this study as a substrate for the�in situ�hydrogel fabrication, due to self-assembly characteristic and a presence of modifiable functional groups. However, regenerated SF solution poses a slow gelation (several days or weeks) which is impractical for an in situ application. Therefore, a phospholipid, dimyristoyl glycerophosphoglycerol (DMPG), and a gold salt (Au3+) were introduced as chemical inducers to accelerate the gelation process of SF. SF hydrogels induced by DMPG can be obtained within 10-40 min depending on the concentration of DMPG. Electrostatic and hydrophobic interactions were proposed as driving forces for inducing SF structural transition. Encapsulated L929 and NIH/3T3 fibroblasts in the hydrogel displayed a normal proliferation, confirming the cytocompatibility of DMPG-induced SF hydrogel. Subsequently, DMPG-based liposomes were prepared and loaded with an anticancer drug, curcumin. The liposomes can enhance loading amount and extend curcumin stability as well as induce the rapid gelation of SF. Curcumin released from liposome-SF hydrogels can inhibit the growth of cancer cells. When using the hydrogel as a substrate for cell culture, low cell survival was observed, possibly due to the combination of low cell attachment on SF and cytotoxicity of curcumin. Moreover, an addition of a gold salt (Au3+) in regenerated and thiolated SF solutions can accelerate the sol-to-gel transition. Dityrosine formation was proposed as an underlying mechanism of gelation for regenerated SF, while the formation of disulfide and Au-S bonds was proposed for thiolated SF. The obtained hydrogels exhibited good cytocompatibility with L929 fibroblasts. In summary, the rapid gelation of SF can be achieved by the addition of DMPG or Au3+�salt. Different gelation mechanisms were proposed, and the obtained hydrogels showed the�in vitro�cytocompatibility, indicating the potential uses as a biomaterial for different biomedical applications.

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Cationic Surfactant-Induced Instantaneous Gelation of Silk Fibroin Solution

Cited by 5 publications

References 16 publications

Tuning Microcapsule Shell Thickness and Structure with Silk Fibroin and Nanoparticles for Sustained Release

Tuning Microcapsule Shell Thickness and Structure with Silk Fibroin and Nanoparticles for Sustained Release

Cooperative Assembly of a Peptide Gelator and Silk Fibroin Afford an Injectable Hydrogel for Tissue Engineering

Phospholipid- and gold-induced gelation of Thai silk fibroin and its applications for cell and drug delivery

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