Cationic supramolecular nanoparticles for co-delivery of gene and anticancer drug

Abstract

Self-assembly supramolecular nanoparticles (SNPs) are synthesized using polyethylenimine (PEI) crosslinked by cyclodextrins (CyDs), adamantyl doxorubicin and plasmid DNA. SNPs successfully conduct the cooperative co-delivery of chemotherapeutic anticancer drug and plasmid DNA in vitro and in vivo.

Materials innovation for co-delivery of diverse therapeutic cargos

“…In line with the challenge of delivering drugs with different properties, there has also been focus on the development of carriers that are able to deliver both a therapeutic drug and a therapeutic nucleic acid 70, 73, 75, 77, 78, 80, 82, 83 . Carriers for co-delivery of a gene and a drug are often conceived for the purposes of overcoming multidrug-resistance in cancer cells.…”

“…Carriers for co-delivery of a gene and a drug are often conceived for the purposes of overcoming multidrug-resistance in cancer cells. Typically, these carriers consist of a hydrophobic core capable of encapsulating a hydrophobic drug and a positively charged, hydrophilic shell capable of complexing the negatively-charged nucleic acids 70, 75, 78, 80, 83 . One group created PEI-PCL nanoparticles that contained doxorubicin in the hydrophobic core and siRNA targeting the BCL-2 gene complexed to PEI.…”

A supramolecular strategy to assemble multifunctional viral nanoparticles

“…12,[22][23][24][25][26][27][28][29] On the other hand, supramolecular interactions have been extensively applied to constructing drug and gene delivery systems, as well as smart materials due to their modularity, reversibility and stimuli-responsiveness. [30][31][32][33][34][35][36] For example, b-cyclodextrin (b-CD), a natural toroid-shaped cyclic oligosaccharide, is one of the most widely used host-systems in supramolecular chemistry thanks to its low cost, good water solubility and biocompatible properties. 37 b-CD has a hydrophilic exterior surface and a hydrophobic interior cavity that can accommodate a broad range of guest molecules (e.g., adamantane, azobenzene, and ferrocene).…”

“…S7, ESI †), similar to the literature reports. 31,32,36 To investigate the selective targeting ability of Dox-loaded TMV, HepG2 tumor cells (folate receptor positive) and NIH-3T3 fibroblast cells (folate receptor negative) were treated with TMV-b-CD/ Ada-Dox, TMV-b-CD/Ada-FA/Ada-Dox and free Dox. Cellular uptake and cell proliferation were evaluated by confocal laser scanning microscopy and cell viability assay, respectively.…”

Amphiphilic graft copolymer based on poly(styrene-co-maleic anhydride) with low molecular weight polyethylenimine for efficient gene delivery

“…[11][12][13][14] Other strategies have included using linear chains to link PEI together, such as grafting PEI to a linear polymer backbone to give comb-shaped copolymers, [15][16][17] conjugating low molecular weight PEI to a biocompatible polymer core to form star-shaped copolymers, 18,19 and constructing supramolecular polymers with grafting of low molecular weight PEI. 20,21 Poly(styrene-co-maleic anhydride) (SMA), an amphiphilic macromolecule, is an intermediate polymer that can be conveniently converted to a functional polymer by reacting with a bioactive agent containing amino or hydroxyl groups via a ring-opening reaction between the active agent and the succinic anhydride unit. SMA has been shown to be biologically safe and to confer immunopotentiation in host animals by activation of macrophages, T cells, and natural killer cells, and induction of interferons.…”