Cationic Polymer Nanoparticles and Nanogels: From Synthesis to Biotechnological Applications

Ramos, José; Forcada, Jacqueline; Hidalgo‐Álvarez, R.

doi:10.1021/cr3002643

Cited by 165 publications

(154 citation statements)

References 458 publications

Supporting

Mentioning

153

Contrasting

Order By: Relevance

“…[ 17,18 ] We have recently introduced a synthetic route to access precise cationic nanohydrogel particles with adjustable sizes for cellular delivery of siRNA: via reversible addition-fragmentation chain transfer (RAFT) block copolymerization of pentafl uorophenyl methacrylate (PFPMA) and tri(ethylene glycol) methyl ether methacrylate (MEO 3 MA), well-defi ned amphiphilic reactive ester block copolymers can be generated that self-assemble in polar aprotic solvents (e.g., dimethyl sulfoxide) into nm-sized micellar aggregates. These precursor structures can permanently be locked-in by a cross-linking reaction of the hydrophobic reactive ester moieties inside the core with spermine affording cationic nanohydrogel particles of different sizes.…”

Section: Introductionmentioning

confidence: 99%

Degradable Cationic Nanohydrogel Particles for Stimuli‐Responsive Release of siRNA

Nuhn

Braun

Overhoff

et al. 2014

Macromol. Rapid Commun.

View full text Add to dashboard Cite

Well-defined nanogels have become quite attractive as safe and stable carriers for siRNA delivery. However, to avoid nanoparticle accumulation, they need to provide a stimuli-responsive degradation mechanism that can be activated at the payload's site of action. In this work, the synthetic concept for generating well-defined nanohydrogel particles is extended to incorporate disulfide cross-linkers into a cationic nanonetwork for redox-triggered release of oligonucleotide payload as well as nanoparticle degradation under reductive conditions of the cytoplasm. Therefore, a novel disulfide-modified spermine cross-linker is designed that both allows disassembly of the nanogel as well as removal of cationic charge from residual polymer fragments. The degradation process is monitored by scanning electron microscopy (SEM) and fluorescence correlation spectroscopy (FCS). Moreover, siRNA release is analyzed by agarose gel electrophoresis and a fluorescent RNA detection assay. The results exemplify the versatility of the applied nanogel manufacturing process, which allows alternative stimuli-responsive core cross-linkers to be integrated for triggered oligonucleotide release as well as effective biodegradation for reduced nanotoxicity.

show abstract

Section: Introductionmentioning

confidence: 99%

Degradable Cationic Nanohydrogel Particles for Stimuli‐Responsive Release of siRNA

Nuhn

Braun

Overhoff

et al. 2014

Macromol. Rapid Commun.

View full text Add to dashboard Cite

show abstract

“…Nanogels are cross-linked polymeric particles in the colloidal range being able to swell by absorption of a suitable solvent, thanks to their cross-linked polymeric network structure. 1 Apart from having temperature-and/or pH-sensitivity, such nanogels can be further labeled with magnetic nanoparticles (MNPs) in the form of socalled magneto-nanogels (MNGs) that exhibit magnetic response to the external stimulus of a magnetic field. Among other advantages, MNPs and MNGs have the multifaceted potential to carry substances, to be magnetically guidable, to absorb toxins from a biochemical medium, to provide inherent contrast under a dc-magnetic field in magnetic resonance imaging, and to produce heat in response to an ac-magnetic field in hyperthermia.…”

mentioning

confidence: 99%

Synthesis and characterization of PDEAEMA‐based magneto‐nanogels: Preliminary results on the biocompatibility with cells of human peripheral blood

Pikabea

Ramos

Papachristos

et al. 2015

J. Polym. Sci. Part A: Polym. Chem.

Self Cite

View full text Add to dashboard Cite

Nanogels based on biocompatible, dual pH-and temperature-sensitive poly(2-(diethylamino)ethyl) methacrylate (PDEAEMA) have been successfully used as nanocontainers for the encapsulation of magnetite, Fe 3 O 4 magnetic nanoparticles (MNPs). For this purpose, citric acid-coated MNPs were encapsulated into previously synthesized PDEAEMA-based nanogels using a poly(ethyleneglycol)-based stabilizer. After the encapsulation of the magnetite MNPs, the so-called magneto-nanogels (MNGs) were proved to be multiresponsive on temperature, pH, and magnetic field and colloidally stable. Moreover, preliminary studies on the biocompatibility of these MNGs with cells of human peripheral blood were performed and evidenced quite tolerable biocompatibility, thus suggesting potential use in biomedical applications.

show abstract

“…Although the synthesis of new stimuli-responsive soft nanoparticles has attracted considerable interest in recent decades, in particular in the case of dual/multi-stimulus-responsive types, practical clinical applications remain limited except for thermo-and pH-sensitive nanoparticles with high sensitivities. 14 In recent years, several attempts to prepare thermo-responsive hybrid micro/nanogels with inorganic silica cores have been reported. [15][16][17][18] These core-shell hybrid nanogels have interesting additional properties compared with polymer nanogels, being promising candidates as controlled drugdelivery vehicles in cancer therapies.…”

Section: Soft Nanoparticlesmentioning

confidence: 99%

Introductory Aspects of Soft Nanoparticles

Estelrich

Quesada‐Pérez

Forcada

et al. 2014

Soft Nanoparticles for Biomedical Applications

Self Cite

View full text Add to dashboard Cite

The aim of this introductory chapter is to present an overview of the book to potential readers. Its four sections summarize the aim of the Editors when the book was planned: to show that research on soft nanoparticles for biomedical applications is a multidisciplinary field where biology, medicine, pharmacy, chemistry and physics meet. In particular, special attention is paid to future improvements in research on nanoparticles that may come from colloidal science and simulation techniques.

show abstract

Cationic Polymer Nanoparticles and Nanogels: From Synthesis to Biotechnological Applications

Cited by 165 publications

References 458 publications

Degradable Cationic Nanohydrogel Particles for Stimuli‐Responsive Release of siRNA

Degradable Cationic Nanohydrogel Particles for Stimuli‐Responsive Release of siRNA

Synthesis and characterization of PDEAEMA‐based magneto‐nanogels: Preliminary results on the biocompatibility with cells of human peripheral blood

Introductory Aspects of Soft Nanoparticles

Contact Info

Product

Resources

About