Cationic lipids enhance siRNA-mediated interferon response in mice

Evaluation of locked nucleic acid–modified small interfering RNAin vitroandin vivo

“…Improved delivery in animals has been achieved by complexation with cationic liposomes (2), polyethylenimine (3), and Arg-Gly-Asp -polyethylene glycol -polyethylenimine (4) or by conjugation of cholesterol to siRNA (5). However, Ma et al (6) has shown that complexation to cationic liposomes resulted in a potent induction of both type I and type II IFN responses in mice. In line, sequencespecific and Toll-like receptor-7 -dependent induction of IFNs has been shown on liposome-mediated siRNA transfection in mice (7).…”

RNA interference and its therapeutic potential

“…All these events show two-fold non-specific effect on the cell system, which include interferon and immune response. There is still a conflict regarding the interferon activity in response to RNAi drug [35,40,45]. Different types of neurological disorders and cancer might occur due to the saturation of RNAi machinery [12,29].…”

RNAi therapeutics: SNALPing siRNAs in vivo

“…[7][8][9][10] Morrissey et al 4 investigated whether or not SNALP delivered, nonbackbone-modified siRNAs were immunostimulatory, and sure enough they were. The fact that the unmodified siRNAs were immunostimulatory indicates that they are subject to endosomal trafficking, where the RNA-activated Toll-like receptors 3 and 7 reside.…”