“…naked plasmid DNA or DNA/liposomes) often lead to transient gene expression when delivered to the mouse lung, despite the persistence of intact plasmid DNA. [40][41][42] Strong viral promoters such as the cytomegalovirus (CMV IE) promoter can become attenuated in the lung, resulting in silencing of transgene expression after only a few days. 41,43 However, as we report here, CMVmediated reporter gene expression from rAAV5/5 is persistent under similar conditions.…”