Cationic detergents enable the separation of membrane proteins of <i>Plasmodium falciparum</i>‐infected erythrocytes by 2D gel electrophoresis

Philipp, Stephan; Jakoby, Thomas; Tholey, Andreas; Janßen, Ottmar; Leippe, Matthias; Gelhaus, Christoph

doi:10.1002/elps.201100520

Cited by 2 publications

(1 citation statement)

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“…It enhances the solubility and recovery of the highly hydrophobic membrane proteins in comparison with the conventional 2D IEF/SDS-PAGE, as well as the low-pH environment which has been aided in the preservation of the unstable methylation of basic proteins [45]. This has been used to successfully separate membrane proteins of Plasmodium falciparum-infected erythrocytes, with the help of cationic detergents such as benzyldimethyl-n-hexadecylammonium chloride (16-BAC) [46]. It has also served in the electrophoresis of mitochondrial membrane proteins of low molecular mass [45].…”

Section: Dimensional Electrophoresis (16-bac/sds-page)mentioning

confidence: 99%

Exploring the World of Membrane Proteins: Techniques and Methods for Understanding Structure, Function, and Dynamics

Boulos,

Jabbour,

Khoury

et al. 2023

Molecules

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In eukaryotic cells, membrane proteins play a crucial role. They fall into three categories: intrinsic proteins, extrinsic proteins, and proteins that are essential to the human genome (30% of which is devoted to encoding them). Hydrophobic interactions inside the membrane serve to stabilize integral proteins, which span the lipid bilayer. This review investigates a number of computational and experimental methods used to study membrane proteins. It encompasses a variety of technologies, including electrophoresis, X-ray crystallography, cryogenic electron microscopy (cryo-EM), nuclear magnetic resonance spectroscopy (NMR), biophysical methods, computational methods, and artificial intelligence. The link between structure and function of membrane proteins has been better understood thanks to these approaches, which also hold great promise for future study in the field. The significance of fusing artificial intelligence with experimental data to improve our comprehension of membrane protein biology is also covered in this paper. This effort aims to shed light on the complexity of membrane protein biology by investigating a variety of experimental and computational methods. Overall, the goal of this review is to emphasize how crucial it is to understand the functions of membrane proteins in eukaryotic cells. It gives a general review of the numerous methods used to look into these crucial elements and highlights the demand for multidisciplinary approaches to advance our understanding.

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Section: Dimensional Electrophoresis (16-bac/sds-page)mentioning

confidence: 99%

Exploring the World of Membrane Proteins: Techniques and Methods for Understanding Structure, Function, and Dynamics

Boulos,

Jabbour,

Khoury

et al. 2023

Molecules

View full text Add to dashboard Cite

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A proteomic glimpse into the effect of antimalarial drugs onPlasmodium falciparumproteome towards highlighting possible therapeutic targets

Dousti

Manzano-Román

Rashidi

et al. 2020

Pathogens and Disease

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There is no effective vaccine against malaria; therefore, chemotherapy is to date only choice to fight against this infectious disease. However, there are growing evidences of drug-resistance mechanisms in malaria treatments. Therefore, the identification of new drug targets is an urgent need for the clinic management of the disease. Proteomic approaches offer the chance of determining the effects of antimalarial drugs on the proteome of Plasmodium parasites. Accordingly, we here review the effects of antimalarial drugs on Plasmodium falciparum proteome pointing out the relevance of several proteins as possible drug targets in malaria treatment. In addition, some of the P. falciparum stage-specific altered proteins and parasite-host interactions might play important roles in pathogenicity, survival, invasion, and metabolic pathways and thus serve as potential source of drug targets. In this review, we have identified several proteins including thioredoxin reductase, helicases, peptidyl-prolyl cis-trans isomerase, endoplasmic reticulum-resident calcium-binding protein, choline/ethanolamine phosphotransferase, purine nucleoside phosphorylase, apical membrane antigen 1, glutamate dehydrogenase, hypoxanthine guanine phosphoribosyl transferase, heat shock protein70x, knob-associated histidine-rich protein, and erythrocyte membrane protein 1 as promising antimalarial drugs targets. Overall, proteomic approaches are able to partially facilitate finding the possible drug targets. However, the integration of other ‘omics’ and specific pharmaceutical techniques with proteomics may increase the therapeutic properties of the critical proteins identified in P. falciparum proteome.

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Cationic detergents enable the separation of membrane proteins of Plasmodium falciparum‐infected erythrocytes by 2D gel electrophoresis

Cited by 2 publications

References 67 publications

Exploring the World of Membrane Proteins: Techniques and Methods for Understanding Structure, Function, and Dynamics

Exploring the World of Membrane Proteins: Techniques and Methods for Understanding Structure, Function, and Dynamics

A proteomic glimpse into the effect of antimalarial drugs onPlasmodium falciparumproteome towards highlighting possible therapeutic targets

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