Cationic and anionic unloaded polymeric nanocapsules: Toxicological evaluation in rats shows low toxicity

Pereira, Muriel Pando; Gomes, Marcelo Gomes de; Izoton, Jessica Cristina; Nakama, Kelly Ayumi; Santos, Raquel Rocha; Savall, Anne Suély Pinto; Ramalho, Juliana Bernera; Roman, Silvane Souza; Luchese, Cristiane; Cibin, Francielli Weber Santos; Pinton, Simone; Haas, Sandra Elisa

doi:10.1016/j.biopha.2019.109014

Cited by 16 publications

(4 citation statements)

References 55 publications

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“…However, reduced serum total protein concentration may be a result of the major effect of drug abuse in reducing the defense system of the body (deposition of immune nephrotic syndrome) thus increasing the risk of infection [43][44][45]. Diazepam metabolites such as oxazepam, desmethyldiazepam, and temazepam have been shown to exert oxidative stress on the kidney leading to total failure [46].…”

Section: оригінальні наукові роботиmentioning

confidence: 99%

Effect of classic soft drink Coca-Cola as a solvent in the administration of tramadol and diazepam on biochemical and histological changes in liver and kidney

Ilesanmi

Odewale

2020

Ukr. J. Nephrol. and Dial.

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The objective of this study was to determine the biochemical and morphological changes in the liver and kidney as a result of the acute administration of tramadol and diazepam with classic soft drink Coca-Cola (Coke ). Method: Thirty-six (36) adult male Wistar rats were divided into six groups: Group A-control (distilled water), Group B (Coke ), Group C (tramadol, 50 mg/kg), Group D (tramadol dissolved in Coke, 50 mg/kg), Group E (diazepam, 10 mg/kg) and Group F (diazepam dissolved in Coke 10 mg/kg). All administrations were done intraperitoneal. Twenty-four hours after administration, blood samples were collected via cardiac puncture for evaluation of the liver (Aspartate aminotransferase [AST] and Alanine aminotransferase [ALT]), kidney (urea and creatinine [CREA]) function and the organs were excised and processed for histopathological examination. Result: A significantly increased in AST, creatinine and urea concentrations was observed in Tramadol and Coke Groups compared to control (P<0.05), while diazepam had no significant effect on AST, ALT (P>0.05), though it caused a significant increase in urea and CREA (P<0.05). Dissolving the tramadol in Coke aggravated its hepatotoxicity and nephrotoxicity, while Coke had no significant effect on diazepam. Histological examination also corroborated the biochemical result. Conclusion: The results showed that mixing drugs with Coke does not improve the toxicity of tramadol and has no significant effect on diazepam.

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Section: оригінальні наукові роботиmentioning

confidence: 99%

Effect of classic soft drink Coca-Cola as a solvent in the administration of tramadol and diazepam on biochemical and histological changes in liver and kidney

Ilesanmi

Odewale

2020

Ukr. J. Nephrol. and Dial.

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“…In living organisms, nanoparticle toxicity is dependent on factors like organ distribution of the particle and retention times, which are mainly determined by nanoparticle features [ 35 ]. A study from Pereira and colleagues (2019), for example, demonstrated that unloaded Eudragit nanocapsules showed no toxic effects in two month old Wistar rats, including no oxidative stress and no hepatic damage markers [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization, Cytotoxicity and Anti-Inflammatory Effect Evaluation of Nanocapsules Containing Nicotine

et al. 2021

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(1) Background: Nanotechnology is an emerging field that can be applied in the biomedical area. In this study, Eudragit nanocapsules (NCs) containing nicotine were produced. Nicotine is the main alkaloid found in tobacco and has anti-inflammatory properties. NCs containing nicotine may be used as an adjuvant therapy in the treatment of inflammation in the central nervous system. (2) Methods: Nanocapsules were prepared by the interfacial deposition of the pre-formed polymer method and characterized in terms of zeta potential, diameter, polydispersity index, pH, encapsulation efficiency (EE), stability and sustained release profile. In vitro tests with the PC12 cell line were performed, such as MTT, LIVE/DEAD and ELISA assays, to verify their cytotoxic and anti-inflammatory effects. (3) Results: The nanocapsules presented satisfactory values of the characterization parameters; however, poor encapsulation was obtained for nicotine (8.17% ± 0.47). The in vitro tests showed that the treatment with nanocapsules reduced cell viability, which suggests that the Eudragit or the amount of polymer on top of the cells may be detrimental to them, as the cells were able to survive when treated with bulk nicotine. ELISA showed an increment in the expression of IL-6 and IL-1β, corroborating the hypothesis that NCs were toxic to the cells because of the increase in the levels of these pro-inflammatory cytokines. (4) Conclusions: This study demonstrates that NCs of Eudragit present toxicity. It is therefore necessary to improve NC formulation to obtain better values for the encapsulation efficiency and reduce toxicity of these nanodevices.

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“…The cytotoxicity of cationic nanomaterials is mainly attributed to the positive charges. Compared with the anion group, cationic nanomaterials exhibit higher cytotoxicity and lethal effects (Calienni et al, 2017;Pereira et al, 2019). Cationic nanomaterials destroy plasma membrane integrity, mitochondrial and lysosomal damage, and more autophagosomes (Mecke et al, 2005;Frohlich 2012).…”

Section: Toxicitymentioning

confidence: 99%

Cationic Nanomaterials for Autoimmune Diseases Therapy

Xie

Huang

et al. 2022

Front. Pharmacol.

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Cationic nanomaterials are defined as nanoscale structures smaller than 100 nm bearing positive charges. They have been investigated to apply to many aspects including clinical diagnosis, gene delivery, drug delivery, and tissue engineering for years. Recently, a novel concept has been made to use cationic nanomaterials as cell-free nucleic acid scavengers and inhibits the inflammatory responses in autoimmune diseases. Here, we highlighted different types of cationic materials which have the potential for autoimmune disease treatment and reviewed the strategy for autoimmune diseases therapy based on cationic nanoparticles. This review will also demonstrate the challenges and possible solutions that are encountered during the development of cationic materials-based therapeutics for autoimmune diseases.

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Cationic and anionic unloaded polymeric nanocapsules: Toxicological evaluation in rats shows low toxicity

Cited by 16 publications

References 55 publications

Effect of classic soft drink Coca-Cola as a solvent in the administration of tramadol and diazepam on biochemical and histological changes in liver and kidney

Effect of classic soft drink Coca-Cola as a solvent in the administration of tramadol and diazepam on biochemical and histological changes in liver and kidney

Characterization, Cytotoxicity and Anti-Inflammatory Effect Evaluation of Nanocapsules Containing Nicotine

Cationic Nanomaterials for Autoimmune Diseases Therapy

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