Cation uptake and allocation by red pine seedlings under cation-nutrient stress in a column growth experiment

Shi, Zhenqing; Balogh‐Brunstad, Zsuzsanna; Grant, M.; Harsh, James B.; Gill, Richard; Thomashow, Linda S.; Dohnálková, Alice; Stacks, Daryl; LeTourneau, Melissa K.; Keller, Catherine

doi:10.1007/s11104-013-2016-2

Cited by 7 publications

(22 citation statements)

References 37 publications

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“…The other two experiments with red pine, which were conducted at Washington State University, are described in Shi et al [49] for the experiment designated as ZS, and in Balogh-Brunstad et al [30] for the experiment designated as BB. Here, only a brief description is provided for these last two experiments, emphasizing the methods that were not previously described and pointing out similarities and differences among the experiments.…”

Section: Methodsmentioning

confidence: 99%

“…Treatment codes and description of treatments used in this paper. KU = University of Copenhagen experiment, ZS = experiment described in Shi et al [49], BB = experiment described in Balogh-Brunstad et al [30]. BB [30] BB_A Abiotic unplanted treatment, both antibiotics and fungicides are used to prevent growth the bacteria and fungi.…”

Section: Methodsmentioning

confidence: 99%

“…Red pine seeds were obtained from Sheffield's Seed Co, Inc., Locke, NY, USA and sterilized with 35% H 2 O 2 . Seed germination and seedling growth were carried out the same way as described in the KU experiment but without initial inoculation [49]. After about three months of growth, the seedlings were transplanted into growth cells and inoculation with soil slurry was carried out there.…”

Section: Seed Germination and Inoculationmentioning

confidence: 99%

“…After about three months of growth, the seedlings were transplanted into growth cells and inoculation with soil slurry was carried out there. The inoculum preparation is described in detail in Shi et al [49]. Briefly, inoculum was prepared from soils and roots collected from the root zone of young Western white pines at 20 miles northeast of Potlatch, ID [49], which is the same location that provided the sporocarps for the BB experiment [30].…”

Section: Seed Germination and Inoculationmentioning

confidence: 99%

“…A new experiment (KU), conducted with Scots pine was inoculated with pure cultures of three ectomycorrhizal fungal species. We also present previously unpublished results from a study [49] conducted with red pine inoculated with a soil slurry (ZS) and from dissertation research [50], where red pine was inoculated with pure cultures of ectomycorrhizal fungi and bacteria associated with those fungi (BB) [30].…”

Section: Introductionmentioning

confidence: 99%

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Ectomycorrhizal Fungi and Mineral Interactions in the Rhizosphere of Scots and Red Pine Seedlings

Balogh‐Brunstad

Keller

Shi

et al. 2017

Soils

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Ectomycorrhizal fungi and associated bacteria play a key role in plant-driven mineral weathering and uptake of mineral-derived nutrients in the rhizosphere. The goal of this study was to investigate the physical and chemical characteristics of bacteria-fungi-mineral interactions in biofilms of Scots and red pine rhizospheres. In three experiments, seedlings were grown in columns containing silica sand amended with biotite and calcium-feldspar, and inoculated with pure cultures of ectomycorrhizal fungi or a soil slurry. Uninoculated seedlings and unplanted abiotic columns served as controls. After nine months, the columns were destructively sampled and the minerals were analyzed using scanning electron and atomic force microscopy. Element release rates were determined from cation concentrations of input and output waters, soil exchange sites, and plant biomass, then normalized to geometric surface area of minerals in each column. The results revealed that various ectomycorrhizal fungal species stimulate silicate dissolution, and biofilm formation occurred at low levels, but direct surface attachment and etching by fungal hyphae was a minor contributor to the overall cation release from the minerals in comparison to other environmental conditions such as water applications (rain events), which varied among the experiments. This research highlights the importance of experimental design details for future exploration of these relationships.

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Section: Methodsmentioning

confidence: 99%