Cation exchange chromatography membrane of Poly(ethylene vinyl alcohol) nanofiber/sulfonated polystyrene microsphere composite for lysozyme isolation

Ji, Cancan; Hu, Wei; Cheng, Peng; Huang, Peng; He, Shan; Xiang, Yang; Zhou, Feng; Liu, Ke; Wang, Dong

doi:10.1016/j.coco.2023.101570

Cited by 6 publications

(3 citation statements)

References 27 publications

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“…As a newly important material, nanofibers with features of super small diameter, high aspect ratio and big specific surface area serve as promising building blocks to fabricate absorption media [13,14]. To date, a series of nanofibers has been assembled into membranes for protein absorption.…”

Section: Introductionmentioning

confidence: 99%

Butane Tetracarboxylic Acid Grafted on Polymeric Nanofibrous Aerogels for Highly Efficient Protein Absorption and Separation

Lu,

Jiang,

Qiao

et al. 2024

Polymers

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Developing high-performance and low-cost protein purification materials is of great importance to meet the demands for highly purified proteins in biotechnological industries. Herein, a facile strategy was developed to design and construct high-efficiency protein absorption and separation media by combining aerogels’ molding techniques and impregnation processes. Poly (ethylene-co-vinyl alcohol) (EVOH) nanofibrous aerogels (NFAs) were modified by grafting butane tetracarboxylic acid (BTCA) over them in situ. This modification was carried out using polyphosphoric acid as a catalyst. The resulting EVOH/BTCA NFAs exhibited favorable comprehensive properties. Benefiting from the highly interconnected porous structure, good underwater compressive properties, and abundant absorption ligands, the obtained EVOH/BTCA NFAs possessed a high static absorption capacity of 1082.13 mg/g to lysozyme and a short absorption equilibrium time of about 6 h. A high saturated dynamic absorption capacity for lysozyme (716.85 mg/g) was also realized solely by gravity. Furthermore, EVOH/BTCA NFAs displayed excellent reusability, good acid and alkaline resistance, and unique absorption selectivity performance. The successful synthesis of such aerogels can provide a potential candidate for next-generation protein absorbents for bio-separation and purification engineering.

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Section: Introductionmentioning

confidence: 99%

Butane Tetracarboxylic Acid Grafted on Polymeric Nanofibrous Aerogels for Highly Efficient Protein Absorption and Separation

Lu,

Jiang,

Qiao

et al. 2024

Polymers

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“…4 Achieving rapid, low-cost, and high-purity separation of lysozyme remains a persistent challenge. 5 Chromatography stands out as one of the most prevalent methods for lysozyme purification, representing a relatively novel approach to biological separation. 6 Among various chromatography technologies, the interaction modes between the ligands and proteins mainly include hydrophobic interactions, ion exchange, sulfur affinity, π−π interactions, and hydrogen bonding.…”

Section: Introductionmentioning

confidence: 99%

“…Over the past few decades, lysozymes have found widespread applications across various fields, including pharmacological technology, where they are utilized in formulations such as wound healing creams, eye drops, and anticancer drugs. , Recent research has also uncovered the potential impact of lysozyme on HIV infection . Achieving rapid, low-cost, and high-purity separation of lysozyme remains a persistent challenge . Chromatography stands out as one of the most prevalent methods for lysozyme purification, representing a relatively novel approach to biological separation …”

Section: Introductionmentioning

confidence: 99%

Nanofibrous Membranes with High Mechanical Strength for the Separation and Purification of Lysozyme

Peng,

Wu,

et al. 2024

ACS Appl. Nano Mater.

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Developing bioseparation media with both high strength and high surface area capable of efficiently separating proteins is a significant challenge. In this study, nanofibrous membranes (NFMs) with mechanical strength up to 10.8 MPa were successfully prepared by using a simple hot pressing and cross-linking process. Subsequently, mixed-mode chromatography (MMC) with both electrostatic and hydrophobic interactions was achieved by introducing glutaric anhydride (GA) onto the surface of the regenerated cellulose (RC) nanofibers. The separation of lysozyme using MMC membranes showed that the static adsorption of lysozyme (1 mg/mL) on RC-g-GA NFM reached 254 mg/g. The dynamic binding capacity of lysozyme in the flow-through mode at 10% breakthrough was 72.8 mg/g. Meanwhile, additionally, the RC-g-GA NFM could saturate lysozyme (3 mg/mL) adsorption up to 336 mg/g after 6 h of adsorption time. Moreover, the membrane could isolate lysozyme with 85% purity from a mixed solution of lysozyme and γ-globulin. This work demonstrates that RC-g-GA NFM has an efficient lysozyme isolation capability and potential for scale-up applications.

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Improving performance of electrospun nylon 6 nanofiber membrane by zeolite nanoparticles for wastewater treatment of herbal essence industries

Hosseini,

Soleimani,

Mirghaffari

et al. 2023

Int. J. Environ. Sci. Technol.

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Cation exchange chromatography membrane of Poly(ethylene vinyl alcohol) nanofiber/sulfonated polystyrene microsphere composite for lysozyme isolation

Cited by 6 publications

References 27 publications

Butane Tetracarboxylic Acid Grafted on Polymeric Nanofibrous Aerogels for Highly Efficient Protein Absorption and Separation

Butane Tetracarboxylic Acid Grafted on Polymeric Nanofibrous Aerogels for Highly Efficient Protein Absorption and Separation

Nanofibrous Membranes with High Mechanical Strength for the Separation and Purification of Lysozyme

Improving performance of electrospun nylon 6 nanofiber membrane by zeolite nanoparticles for wastewater treatment of herbal essence industries

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