Cation effects on phosphatidic acid monolayers at various pH conditions

Zhang, Ting; Cathcart, Matthew G.; Vidalis, Andrew S.; Allen, Heather C.

doi:10.1016/j.chemphyslip.2016.06.001

Cited by 21 publications

(48 citation statements)

References 59 publications

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“…DPPA has been shown to be highly surface active at all ionization states from (0) to (−2), with no change in solubility observed as its ionization state changes, unlike its single chain variants Lyso‐phosphatidic acid (unpublished data) and single chain fatty acids that display increased solubility as they become more negatively charged. As shown in our previous work, BAM images of DPPA in the condensed phase (>0 mN/m surface pressure) revealed a homogenous film covering the water surface (Zhang et al, ). In addition, DPPA is very stable at the air‐water interface, allowing us to treat the DPPA monolayer as a solid phosphoric acid‐water interface.…”

Section: Resultssupporting

confidence: 64%

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Trace Metal Enrichment Driven by Phosphate Functional Group Binding Selectivity

2018

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Trace metals have been found to be selectively enriched in the sea surface microlayer (SSML) due to association with organics, but the underlying cause of this selectivity has not been as well studied. Therefore, a systematic cation affinity study on surfactant lipids containing various functional headgroups (‐OH, ‐H2PO4, ‐COOH, ‐N(CH3)2) was conducted at pH 3 to further explain cation enrichment selectivity using Brewster angle microscopy, surface tension salt titration, and infrared‐reflection absorption spectroscopy (IRRAS). At pH 3, the headgroups chosen are neutral, and therefore the variability of surface charge on binding is eliminated. The phosphate ester headgroup was observed to exhibit the strongest trace metal binding, followed by the carboxylic acid headgroup. Hydroxy and dimethylamine headgroups did not exhibit significant trace metal binding. By applying the Langmuir‐Szyszkowski model to the surface tension data, the cation surface binding affinities for phosphatidic acid were determined, with Al3+ > Fe3+ > Zn2+ > Mg2+ > Ni2+ > Mn2+ ∼ Ca2+, an order not predicted from bulk properties such as solid formation constants of metal‐phosphonates. Thus cation binding to surface active organic molecules with phosphate ester headgroups is indicated to be a significant source of trace metal enrichment within the SSML.

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Section: Resultssupporting

confidence: 64%

“…DPPA has been well documented in our previous works as an ocean relevant organic surfactant with high surface activity and a simple phosphate headgroup (Zhang et al, 2016(Zhang et al, , 2017. In this study, it remains our representative phosphoric acid lipid model.…”

Section: Surface Tension Salt Titrations: Cation Selectivity Between mentioning

confidence: 84%

Trace Metal Enrichment Driven by Phosphate Functional Group Binding Selectivity

2018

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“…The binding of Ca 2+ to lipid membranes has been the focus of extensive study 18, 39–45 . Ca 2+ has been shown to weakly associate with zwitterionic membranes, while it interacts strongly with membranes containing negatively charged lipids 45 .…”

Section: Resultsmentioning

confidence: 99%

Cations induce shape remodeling of negatively charged phospholipid membranes

Graber

Shi

Baumgart

2017

Phys. Chem. Chem. Phys.

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The divalent cation Ca2+ is a key component in many cell signaling and membrane trafficking pathways. Ca2+ signal transduction commonly occurs through interaction with protein partners. However, in this study we show a novel mechanism by which Ca2+ may impact membrane structure. We find an asymmetric concentration of Ca2+ across the membrane triggers deformation of membranes containing negatively charged lipids such as Phosphatidylserine (PS) and Phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2). Membrane invaginations in vesicles were observed forming away from the leaflet with higher Ca2+ concentration, showing that Ca2+ induces negative curvature. We hypothesize that the negative curvature is produced by Ca2+-induced clustering of PS and PI(4,5)P2. In support of this notion, we find that Ca2+-induced membrane deformation is stronger for membranes containing PI(4,5)P2, which is known to more readily cluster in the presence of Ca2+. The observed Ca2+-induced membrane deformation is strongly influenced by Na+ ions. A high symmetric [Na+] across the membrane reduces Ca2+ binding by electrostatic shielding, inhibiting Ca2+-induced membrane deformation. An asymmetric [Na+] across the membrane, however, can either oppose or support Ca2+-induced deformation, depending on the direction of the gradient in [Na+]. At a sufficiently high asymmetric Na+ concentration it can impact membrane structure in the absence of Ca2+. We propose that Ca2+ works in concert with curvature generating proteins to modulate membrane curvature and shape transitions. This novel structural impact of Ca2+ could be important for Ca2+-dependent cellular processes that involve the creation of membrane curvature, including exocytosis, invadopodia, and cell motility.

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“…Phosphatidic acid, LPA and DAG are involved in many membrane fusion and membrane fission events, such as fission of Golgi membranes , fission during endocytic transport , vacuole fission and fusion , mitochondrial dynamics , exocytosis , vesicle‐vesicle fusion , myoblast fusion , osteoclast fusion , membrane fusion during sporulation , membrane fusion during fertilization . Fusion of PA‐containing vesicles was studied in Refs .…”

Section: The Role Of Pa In Membrane Fusion and Membrane Fissionmentioning

confidence: 99%

“…Phosphatidic acid, LPA and DAG are involved in many membrane fusion and membrane fission events, such as fission of Golgi membranes [140,162,163,[165][166][167][168][169], fission during endocytic transport [170,171], vacuole fission [172] and fusion [132,147,[173][174][175], mitochondrial dynamics [17,40,95,[176][177][178][179][180][181][182][183][184][185], exocytosis [30,40,76,79,125,[186][187][188][189][190][191][192][193][194], vesicle-vesicle fusion [164], myoblast fusion [195,196], osteoclast fusion [197], membrane fusion during sporulation [198][199][200][201], membrane fusion during fertilization [202,203]. Fusion of PA-containing vesicles was studied in Refs [87,…”

Section: The Role Of Pa In Membrane Fusion and Membrane Fissionmentioning

confidence: 99%

Phosphatidic acid in membrane rearrangements

et al. 2019

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Phosphatidic acid (PA) is the simplest cellular glycerophospholipid characterized by unique biophysical properties: a small headgroup; negative charge; and a phosphomonoester group. Upon interaction with lysine or arginine, PA charge increases from −1 to −2 and this change stabilizes protein–lipid interactions. The biochemical properties of PA also allow interactions with lipids in several subcellular compartments. Based on this feature, PA is involved in the regulation and amplification of many cellular signalling pathways and functions, as well as in membrane rearrangements. Thereby, PA can influence membrane fusion and fission through four main mechanisms: it is a substrate for enzymes producing lipids (lysophosphatidic acid and diacylglycerol) that are involved in fission or fusion; it contributes to membrane rearrangements by generating negative membrane curvature; it interacts with proteins required for membrane fusion and fission; and it activates enzymes whose products are involved in membrane rearrangements. Here, we discuss the biophysical properties of PA in the context of the above four roles of PA in membrane fusion and fission.

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Cation effects on phosphatidic acid monolayers at various pH conditions

Cited by 21 publications

References 59 publications

Trace Metal Enrichment Driven by Phosphate Functional Group Binding Selectivity

Trace Metal Enrichment Driven by Phosphate Functional Group Binding Selectivity

Cations induce shape remodeling of negatively charged phospholipid membranes

Phosphatidic acid in membrane rearrangements

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