Cation and Metabolic Requirements for Retention of Metaraminol by Rat Uterine Horns

Paton, David M.

doi:10.1111/j.1476-5381.1968.tb00989.x

Cited by 21 publications

(14 citation statements)

References 14 publications

(24 reference statements)

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“…Transport of noradrenaline and metaraminol has been generally reported to be K+ dependent (Gillis & Paton, 1967;Colburn, et al, 1968 ;Paton, 1968;Sugrue & Shore, 1969a) although the uptake of [3H]-noradrenaline by perfused cat spleen was reported not to require K+ (Kirpekar & Wakade, 1968). This finding may have resulted from a failure adequately to deplete the tissue of K+ before determining the magnitude of amine uptake.…”

Section: Discussioncontrasting

confidence: 43%

“…Tissues accumulate noradrenaline and structurally related compounds such as metaraminol, by a process which is temperature dependent, requires energy, is Na+ and K+ dependent and is inhibited by ouabain (Dengler, Michaelson, Spiegel & Titus, 1962;Giachetti & Shore, 1966;Iversen & Kravitz, 1966;Gillis & Paton, 1967;Colburn, Goodwin, Murphy, Bunney & Davis, 1968;Paton, 1968;Wakade & Furchgott, 1968). These findings suggest that the Na+ and K+ activated adenosine triphosphatase (membrane ATPase) is involved in the transport of noradrenaline.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Na⁺ and K⁺ on the uptake of metaraminol by rabbit ventricular slices

Paton

1971

British J Pharmacology

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4. The inhibitory effects on transport of either a marked reduction in the concentration of Na+ or of omission of K+ were rapidly reversible on exposure of slices to Krebs solution. 5. The inhibitory effect of ouabain on [3H]-metaraminol transport was markedly time dependent, being significantly increased by preincubation with ouabain. The inhibitory action of ouabain was not affected, however, by the Na+ concentration present during preincubation. 6. An inwardly directed Na+ gradient did not increase [3H]-metaraminol transport in slices in which Na+ pumiping had been prevented by 1O-5M ouabain, 40 C, omission of K+ or by metabolic inhibition. 7. These findings provide additional evidence that the Na+ and K+ activated adenosine triphosphatase may participate in the transport of metaraminol and related amines. In the absence of Na+ pumping, induced Na+ gradients were unable to produce transport of amine as would be predicted by the co-transport model proposed by . IntroductionTissues accumulate noradrenaline and structurally related compounds such as metaraminol, by a process which is temperature dependent, requires energy, is Na+ and K+ dependent and is inhibited by ouabain (Dengler, Michaelson, Spiegel &

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Section: Discussioncontrasting

confidence: 43%

Section: Introductionmentioning

confidence: 99%

Effects of Na⁺ and K⁺ on the uptake of metaraminol by rabbit ventricular slices

Paton

1971

British J Pharmacology

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“…A possible interaction between potassium and tissue neural elements is suggested by the fact that the accumulation of various tissue amines is altered in a complex fashion by potassium ion (41)(42)(43). As an indication that this effect may be a real factor in the microvascular response to potassium, Sudak and Fulton (44) found that the cheek pouch vasculature constricts when the plasma potassium concentration is increased.…”

Section: Dulingmentioning

confidence: 99%

Effects of potassium ion on the microcirculation of the hamster.

Duling¹

1975

Circulation Research

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The vasoactive properties of potassium were assessed in the microcirculation of the hamster cremaster muscle and the muscular and epithelial portions of the hamster cheek pouch. Tissues were transilluminated and suffused with a physiological salt solution whose potassium concentration varied from 0 to 20 mM. Vessel diameters were measured and normalized as a percent of the control diameter (± SE) observed during exposure to 4.7 mM K + . Altering the potassium concentration in the suffusion solution caused a transient vascular response. The peak changes in the vascular diameter of the arterioles supplying striated muscle varied directly with the suffusion solution potassium concentration from a minimum of 78 ± 3% in 0 mM K + to 155 ± 15% in 15 mM K". Vascular diameter increases were sustained for the full 5-minute test period only in 15 mM K+. In the epithelial portions of the cheek pouch, only the constrictor component of the potassium response was observed. The data indicate that potassium is sufficiently potent to participate in initiating functional hyperemia in striated muscle and might cause as much as a 6.3-fold increase in flow. Functional hyperemias exceeding approximately 3 minutes cannot be due to potassium ion, since the dilation induced by this agent is transient.• Cells contain a large store of labile potassium that can be released by various stimuli such as muscle contraction and hypoxia (1-9). Many investigators have shown that potassium at the increased concentrations observed in venous blood during exercise and hypoxia can relax smooth muscle both in vivo (5)(6)(7)(8)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) and in vitro (21-31). Therefore, this ion. may be important in the local control of blood flow. However, further quantification of the reactivity of resistance vessels to the ion must be carried out before accurate estimates of the importance of potassium in blood flow regulation can be made. These data can be obtained by studying microvessel responses to alterations in suffusion solution potassium concentrations, but such experiments have only been performed on pial vessels (11,16,17). Furthermore, no data are currently available showing the temporal response pattern of microvessels during variations in potassium concentration. These data are of particular interest in view of the transient potassium ion release during functional hyperemia in striated muscle (7,8) and the transient vascular smooth muscle response to potassium both in vitro (26-32) and in vivo (10). A preliminary report of this work was presented at the Fall Meeting of the American Physiological Society, August, 1974. This work was done during Dr. Duling's tenure as an Established Investigator of the American Heart Association.Received September 20, 1974. Accepted for publication June 4, 1975. I have therefore studied the responses of the arterioles of the hamster cremaster muscle and cheek pouch to changes in the potassium concentration of a superfusion solution. The aims of the experiment were to: (1) estab...

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“…Much evidence has accumulated that sympathetic postganglionic neurones concentrate noradrenaline and certain structurally-related com pounds such as metaraminol, from the external medium [11,13] by a membrane transport process which is temperature-sensitive [4,7], in hibited by ouabain [4,18] and is Na+-and K+-dependent [1,6,[16][17][18]. In addition, uptake of the amine requires energy [16,22].…”

Section: Rabbit Atria Rabbit Vas Deferens Glycolysis Substrates Noradmentioning

confidence: 99%

“…In addition, uptake of the amine requires energy [16,22]. The purpose of the present study was to examine the energy requirements for the uptake of (3H)-noradrenaline by long and short peripheral adrenergic neurones present in the atria and vas deferens, respectively [15], and to compare these findings with related studies of amine uptake by rat [7,8,16], guinea pig [22], amphibian [17], and embryonic chick tissues [10]. …”

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confidence: 99%

Metabolic Requirements for the Uptake of Noradrenaline by Isolated Atria and Vas Deferens of the Rabbit

Paton¹

1972

Pharmacology

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The metabolic requirements for the accumulation of (³H)-noradrenaline by rabbit atria and vas deferens have been studied. It was found that inhibition of either glycolysis or oxidation via the Krebs cycle alone had little effect on amine accumulation but inhibition of both processes greatly reduced accumulation of (³H)-noradrenaline by both tissues. Glycolysis was inhibited by iodoacetate and 2-deoxy-D-glucose. D-Glucose, D-mannose, pyruvate, lactate, oxaloacetate and β-hydroxy-butyrate could be utilised for the energy requirements of amine transport but D-galactose, D-fructose, 2-deoxy-D-glucose, 3-O-methyl-glucose and succinate were not.

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Cation and Metabolic Requirements for Retention of Metaraminol by Rat Uterine Horns

Cited by 21 publications

References 14 publications

Effects of Na⁺ and K⁺ on the uptake of metaraminol by rabbit ventricular slices

Effects of Na⁺ and K⁺ on the uptake of metaraminol by rabbit ventricular slices

Effects of potassium ion on the microcirculation of the hamster.

Metabolic Requirements for the Uptake of Noradrenaline by Isolated Atria and Vas Deferens of the Rabbit

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Cation and Metabolic Requirements for Retention of Metaraminol by Rat Uterine Horns

Cited by 21 publications

References 14 publications

Effects of Na+ and K+ on the uptake of metaraminol by rabbit ventricular slices

Effects of Na+ and K+ on the uptake of metaraminol by rabbit ventricular slices

Effects of potassium ion on the microcirculation of the hamster.

Metabolic Requirements for the Uptake of Noradrenaline by Isolated Atria and Vas Deferens of the Rabbit

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Effects of Na⁺ and K⁺ on the uptake of metaraminol by rabbit ventricular slices

Effects of Na⁺ and K⁺ on the uptake of metaraminol by rabbit ventricular slices