Cathodoluminescence of biological molecules, macromolecules and cells

Barnett, W.A.; Wise, M. L. H.; Jones, Emma R.

doi:10.1111/j.1365-2818.1975.tb04063.x

Cited by 18 publications

(5 citation statements)

References 19 publications

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“…Generally, SEM samples are sputter-coated with gold/palladium (or similar metals) to avoid a charge build-up on the sample surface and subsequent saturation of the electron detector. However, like other workers [18, 19, 20], we have noted almost complete loss of fluorescence with a 60-second gold/palladium coating. We found that a 15-second sputter coating passes at least 50% of the maximum fluorescence.…”

Section: Sample Preparationsupporting

confidence: 90%

Immuno-Fluorescence Scanning Electron Microscopy of Biological Cells

et al. 2010

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Scanning electron microscopy (SEM) can produce striking three-dimensional images of biological cells and tissues with submicron resolution of surface morphology. Such cell surfaces are often complex blends of folds, extrusions, and pockets that may be necessary in the positioning of specific molecules within interaction range of each other. Thus, surface changes can have a spatial control over some molecular functions, and identification of select molecules at distinct morphological locations becomes critical to our understanding of total cell function.

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Section: Sample Preparationsupporting

confidence: 90%

Immuno-Fluorescence Scanning Electron Microscopy of Biological Cells

et al. 2010

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“…Organic molecules, which comprise up to 3% of conodont elements (Zhuravlev, 2023), may make some contribution to CL. Pure hydroxyapatite (HAP) has a grey or reddish CL, but pure collagen has a bluish-green CL (Barnett et al, 1975;Roman-Lopez et al, 2014). In RGB coordinates, the CL of all tissues of unaltered conodont elements is close to that of HAP and different from that of pure collagen (fig.…”

Section: Discussionmentioning

confidence: 99%

Cathodoluminescence of conodont elements

Zhuravlev

2023

Vestnik of Geosciences

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Conodont elements are used as a geochemical archive of seawater. Some compositional features of conodont elements reflect conodont ecology and trophic structure of Palaeozoic pelagic ecosystems. However, the screening of conodont elements prior to geochemical and/or isotopic studies is a real problem. This study evaluates SEM cathodoluminescence (SEM-CL), which is very sensitive to the REE and Mn content of apatite, for the detection of traces of secondary transformation in the composition of conodont bioapatite. The SEM-CL of conodont elements is similar to that of unaltered shark teeth (blue-violet), but differs significantly from that of fossil vertebrate teeth (orange-red). Thermal alteration has little effect on the SEM-CL. Elements with a CAI of 1—1.5 show a redder and more intense CL than elements with a CAI of 5. In the case of corrosion of the conodont element surface in carbonate host rocks, the CL of the outer parts of the conodont element become reddish due to invasion of the carbonate material. Conodont elements from the clay host rock show deep purple SEM-CL. Thus, SEM-CL allows detection of the results of secondary processes in conodont mineralised tissues, including enrichment by REE and/or Mn, corrosion and contamination by carbonate material. This method can be used to screen significantly altered samples prior to chemical and isotopic analyses.

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“…Structural analysis of the functional units is performed by electron microscopy, and the relationships between the structures and their functions are investigated. − The CL imaging, using a scanning electron microscope (SEM), can resolve the limitation of the spatial resolution, which is restricted by the Rayleigh criterion. Imaging of the organic compounds, such as synthetic dyes and biological materials, has been challenged using CL. − Therefore, the development of a organic luminescent probe with high emission efficiency and high electron irradiation tolerance is expeditiously demanded.…”

Section: Introductionmentioning

confidence: 99%

Cathodoluminescence and Electron-Induced Fluorescence Enhancement of Enhanced Green Fluorescent Protein

et al. 2016

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Becaues the spatial resolution of fluorescence microscopy is not high enough to study the molecular level of relationship between the structure and function of biological specimens, correlative light and electron microscopy has been used for this purpose. Another possibility for a high-resolution light microscopy is cathodoluminescence microscopy. Here, we report a new phenomenon, the electron-induced activation of luminescence (cathodoluminescence) and electron-enhanced fluorescence for the enhanced green fluorescent protein (EGFP). This was found using our recently developed hybrid fluorescence and electron microscopy. Contrary to the past reports, which showed a degradation of organic compounds by electron irradiation, stable cathodoluminescence emitted from an organic molecule, EGFP, has been observed using the hybrid microscopy. Addition of the glycerol promoted the fluorescence enhancement of EGFP probably due to the change in the electronic state density of excitation channels from the ground to the excited state or of relaxation channels from the excited to the emission state. Stable cathodoluminescence and enhanced fluorescence of the EGFP may introduce a cathodoluminescence microscopy, which will increase the variety of the imaging to investigate the biological compounds.

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Cathodoluminescence of biological molecules, macromolecules and cells

Cited by 18 publications

References 19 publications

Immuno-Fluorescence Scanning Electron Microscopy of Biological Cells

Immuno-Fluorescence Scanning Electron Microscopy of Biological Cells

Cathodoluminescence of conodont elements

Cathodoluminescence and Electron-Induced Fluorescence Enhancement of Enhanced Green Fluorescent Protein

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