Cathinone, an active principle of Catha edulis, accelerates oxidative stress in the limbic area of swiss albino mice

Safhi, Mohammed M.; Alam, Mohammad Firoz; Hussain, Sohail; Siddiqui, Mohammed Abdul Hakeem; Khuwaja, Gulrana; Khardali, Ibrahim A.; Al-Sanosi, Rashad Mohammed; Islam, Fakhrul

doi:10.1016/j.jep.2014.08.004

Cited by 17 publications

(14 citation statements)

References 25 publications

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“…Current research is an extension of our previous work (1,4) where the effects of cathinone on the lipids (1) and oxidative stress (4) in the limbic areas of Swiss albino mice were investigated. Now, behaviors of Swiss albino mice and contents of dopamine and its metabolites are in the focus of our interest.…”

mentioning

confidence: 89%

“…Groups 2 and 3 were given cathinone 1.25 and 2.5 mg kg -1 i.p. once daily for a period of 15 days as reported (1,4). On day 13, 1 h after cathinone dosing, the locomotor activity was observed for 30 min in each group using an actophotometer.…”

Section: Experimental Designmentioning

confidence: 99%

“…On the last day of experiment (day 15), one hour after the dosing, the mice were sacrificed and brains were taken out quickly to dissect the limbic area for the estimation of dopamine and its metabolite. Brain portion between the posterior optic chiasma and anterior brain stem was used as limbic area as described earlier (1). The limbic area (20 %, m/V) was sonicated in perchloric acid (0.4 mol L -1 ) with internal standard (3,4-dihydroxybenzylamine, 100 ng mL -1 ) and centrifuged at 15,000xg for 10 min at 4 °C.…”

Section: Dissection Of the Limbic Areas And Isolation Of Neurotransmimentioning

confidence: 99%

“…Qureshi et al (3) have given 5, 20 and 40 mg kg -1 bm of cathinone orally to male albino mice for a period of 6 weeks. Safhi et al (1,4) have administered 0.125, 0.25 and 0.5 mg kg -1 bm of cathinone i.p. to Swiss albino mice for a period of 15 days.…”

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confidence: 99%

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Catha Edulis Active Principle, Cathinone, Suppresses Motor Coordination, Accelerates Anxiety and Alters the Levels of Dopamine and its Metabolites in the Limbic Areas of Male Swiss Albino Mice

et al. 2018

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Cathinone, the active principle of khat (Catha edulis), stimulates, excites and produces euphoric feelings in khat users. Locomotor and rearing activities, either individual or in groups, of male Swiss albino mice were decreased significantly compared to the control. Motor coordination tests (rotarod, rope climb and grip tests) have shown decreased motor performance in the mice treated with cathinone compared to the control. The elevated plus maze test has shown significant anxiety in the mice compared to the control. Contents of dopamine and its metabolite, homovanillic acid, were increased in the limbic areas compared to the control group. In contrast, contents of 3,4-dihydroxyphenyl acetic acid were depleted significantly and dose dependently compared to the control group in the limbic areas of mice. In conclusion, natural cathinone has depleted motor coordination, accelerated anxiety in mice and altered the contents of dopamine and its metabolites.

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mentioning

confidence: 89%

Section: Experimental Designmentioning

confidence: 99%

Section: Dissection Of the Limbic Areas And Isolation Of Neurotransmimentioning

confidence: 99%

mentioning

confidence: 99%

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Catha Edulis Active Principle, Cathinone, Suppresses Motor Coordination, Accelerates Anxiety and Alters the Levels of Dopamine and its Metabolites in the Limbic Areas of Male Swiss Albino Mice

et al. 2018

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“…In addition, khat has been reported to be a psychostimulant ( 10 ). Medicinal uses of Khat for the treatment of depression, hunger, fatigue, obesity and gastric ulcers have also been previously reported ( 11 ). Khat can affect the cardiovascular, digestive, endocrine, hepatobiliary, respiratory and genitourinary systems ( 12 ).…”

Section: Introductionmentioning

confidence: 95%

Khat promotes human breast cancer MDA-MB-231 cell apoptosis via mitochondria and MAPK-associated pathways

Xiang

et al. 2017

Oncology Letters

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Khat (Catha edulis Forsk) is a flowering evergreen plant in Eastern Africa and Southwestern Arabia. Consumption of Khat has been associated with the development of oral cancer, but its mechanism of action on the molecular level remains unclear. The present study demonstrated the cytotoxic effect of khat extracts on the human breast cancer cell line MDA-MB-231. Trypan blue exclusion assays, flow cytometry, fluorescent and electron microscopy, as well as western blotting were used to analyze the effects of Khat on the cell viability of breast cancer cells, expression of apoptotic-associated proteins and the levels of reactive oxygen species (ROS). The results of the present study demonstrated that treatment with 400 µg/ml khat was able to induce cell death in breast cancers, with an increase in the protein expression of apoptosis regulator Bax and a decrease in the expression of B-cell lymphoma 2, along with a decrease in ROS levels in a time-dependent manner. Furthermore, the expression of activated c-Jun N-terminal and extracellular regulated protein kinases was increased in khat-treated cells compared with untreated cells. Mitochondria participated in cell apoptosis through the release of apoptogenic proteins to the cytosol and the generation of excess reactive oxygen species. The results of the present study suggest that khat induces MDA-MB-231 cell apoptosis via MAPK activation and mitochondrial-mediated death.

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Adverse outcome pathways induced by 3,4-dimethylmethcathinone and 4-methylmethcathinone in differentiated human SH-SY5Y neuronal cells

et al. 2020

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Cathinone, an active principle of Catha edulis, accelerates oxidative stress in the limbic area of swiss albino mice

Cited by 17 publications

References 25 publications

Catha Edulis Active Principle, Cathinone, Suppresses Motor Coordination, Accelerates Anxiety and Alters the Levels of Dopamine and its Metabolites in the Limbic Areas of Male Swiss Albino Mice

Catha Edulis Active Principle, Cathinone, Suppresses Motor Coordination, Accelerates Anxiety and Alters the Levels of Dopamine and its Metabolites in the Limbic Areas of Male Swiss Albino Mice

Khat promotes human breast cancer MDA-MB-231 cell apoptosis via mitochondria and MAPK-associated pathways

Adverse outcome pathways induced by 3,4-dimethylmethcathinone and 4-methylmethcathinone in differentiated human SH-SY5Y neuronal cells

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