Catheter-Related Bacteremia Caused by
            <i>Staphylococcus pseudintermedius</i>
            Refractory to Antibiotic-Lock Therapy in a Hemophilic Child with Dog Exposure

Chuang, Chia-Yunn; Yang, Yung‐Li; Hsueh, Po-Ren; Lee, Ping–Ing

doi:10.1128/jcm.02033-09

Cited by 76 publications

(51 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Additionally, it can cause infections in humans (Van Hoovels et al 2006;Chuang et al 2010). Since 2006, methicillin-resistant S. pseudintermedius (MRSP) has emerged as a significant problem in veterinary medicine, due to its clonal spread in Europe and North America (Perreten et al 2010;Weese & van Duijkeren 2010).…”

Section: Introductionmentioning

confidence: 99%

Emergence of biofilm-producingStaphylococcus pseudintermediusisolated from healthy dogs in South Korea

Han

Yang

Park

2015

Veterinary Quarterly

View full text Add to dashboard Cite

Background: Staphylococcus pseudintermedius is a common bacterial pathogen in dogs with pyoderma, otitis externa, and wound infection. Objective: The purpose of this study was to evaluate the biofilm-producing ability for methicillin-resistant (MRSP) and methicillin-susceptible (MSSP) S. pseudintermedius isolates from healthy dogs. Animals and methods: Ninety-two isolates, consisting of 40 MRSPs and 52 MSSPs, were included in the study. Biofilm production was determined using a quantitative adherence assay in 96-well tissue culture plates and polymerase chain reaction (PCR) targeting icaA and icaD genes. The prevalence of methicillin-resistance in the isolated S. pseudintermedius was calculated using standard equations. The presence of methicillin resistance (MR) and biofilm-producing ability between nasal-and ocular-origin S. pseudintermedius was compared using the chi-square (x 2 ) test. The associations between the degree of biofilm production and the presence of methicillin-resistance or sample origin were determined using Fisher's exact test. Results: All of the MRSP and MSSP isolates were able to produce biofilms, and 93.5% of the isolates were strong or moderate biofilm producers, regardless of the presence of methicillin-resistance or source. Methicillin-resistance was more prevalent in nasal isolates. There was no association between the presence of icaA/icaD and biofilm production. Conclusions: Findings from this study indicate a high prevalence of biofilm-producing S. pseudintermedius in healthy dogs in South Korea, which may have a significant influence to animal health.

show abstract

Section: Introductionmentioning

confidence: 99%

Emergence of biofilm-producingStaphylococcus pseudintermediusisolated from healthy dogs in South Korea

Han

Yang

Park

2015

Veterinary Quarterly

View full text Add to dashboard Cite

show abstract

“…17 Nevertheless, reports of MRSP infections in humans have been documented in the literature. [18][19][20][21][22] The purpose of this study was to compare the prevalence of meticillin-resistant Staphylococcus (MRS) isolation from dogs with first-time or recurrent pyoderma from a primary care facility with those from a tertiary care facility, and to identify risk factors that might be associated with isolation of MRS. We hypothesized that a higher prevalence of MRS would be found in dogs seen at a tertiary care facility than in dogs seen at a primary care facility.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of and Risk Factors for Isolation of Meticillinresistant Staphylococcus Spp. from Dogs with Pyoderma in Northern California, Usa

Eckholm

Outerbridge

White

et al. 2013

Advances in Veterinary Dermatology

View full text Add to dashboard Cite

“…Methicillin-resistant Staphylococcus pseudintermedius (MRSPs) are the frequent cause of pyoderma, urinary tract infections and otitis (Borjesson et al 2012;Kadlec et al 2010;Rubin et al 2011). As an opportunistic pathogen, these gram-positive Staphylococcus pseudintermedius cocci can occasionally cause infections in humans (Chuang et al 2010;Hoovels et al 2006). Due to the emergence and widespread dissemination of MRSP, it becomes an expensive and a serious challenge to control or eradicate these pathogenic biofilms using antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Atomic force microscopy study of the antibacterial effect of fosfomycin on methicillin-resistant Staphylococcus pseudintermedius

Neethirajan

DiCicco

2013

Appl Nanosci

View full text Add to dashboard Cite

The influence of fosfomycin on methicillinresistant Staphylococcus pseudintermedius (MRSP) as the target cell was studied by atomic force microscopy (AFM). Nanoscale images of the effects of the antibiotic fosfomycin on this gram-positive bacterium's cell were obtained in situ without fixing agents. Our study has demonstrated substantial morphological and topographical differences between the control and fosfomycin-treated MRSP cells. The AFM investigations further revealed the rough surface morphology and a 30 % shrinkage in size of the fosfomycin-treated cell and the leakage of cytoplasmic components from the cell. The damage of cell membrane integrity and the cell surface degradation as observed elaborates the antibacterial activity of fosfomycin. The AFM image analysis also reveals that the fosfomycin inhibits cell division, and prevents the adhesion on the surface discouraging the biofilm attachment. The microtitre plate assay results conform to the atomic force microscopy image analysis. This is the first visual demonstration of the effect of fosfomycin on MRSP cells.

show abstract

Catheter-Related Bacteremia Caused by Staphylococcus pseudintermedius Refractory to Antibiotic-Lock Therapy in a Hemophilic Child with Dog Exposure

Cited by 76 publications

References 8 publications

Emergence of biofilm-producingStaphylococcus pseudintermediusisolated from healthy dogs in South Korea

Emergence of biofilm-producingStaphylococcus pseudintermediusisolated from healthy dogs in South Korea

Prevalence of and Risk Factors for Isolation of Meticillinresistant Staphylococcus Spp. from Dogs with Pyoderma in Northern California, Usa

Atomic force microscopy study of the antibacterial effect of fosfomycin on methicillin-resistant Staphylococcus pseudintermedius

Contact Info

Product

Resources

About