Cathepsind activity in isolated odontoblasts

Linde, Anders; Persliden, B

doi:10.1007/bf02012763

Cited by 31 publications

(12 citation statements)

References 33 publications

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“…The localization of high activity of cathepsin D in the dentinogenic region [12] is strong evidence for an important function in the degradation of PGs taking place simultaneously with crystal formation at the mineralization front. The results of Poole et al [27] indicate that this process occurs also in bone formation.…”

Section: Discussionmentioning

confidence: 98%

Cathepsin D: Ultra-immunohistochemical localization in dentinogenesis

Nygren

Persliden

et al. 1979

Calcif Tissue Int

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Cathepsin D was purified from rat liver using a new affinity chromatographic method, based on the coupling to the specific inhibitor pepstatin. This preparation was used for the production of specific antibodies from rabbit. The purified IgG fraction was conjugated to horseradish peroxidase in a two-step coupling procedure and used for electron microscopic immunohistochemistry of the odontoblast-predentine region of the rat incisor. Precipitates, indicating the presence of cathepsin D, were seen in the odontoblast, odontoblast process, and in the extracellular unmineralized matrix, the predentine. The observations are discussed in relation to proteoglycan degradation at the mineralization front simultaneous with crystal formation, and in relation to the function of lysosomal enzymes in the turnover of connective tissue.

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Section: Discussionmentioning

confidence: 98%

Cathepsin D: Ultra-immunohistochemical localization in dentinogenesis

Nygren

Persliden

et al. 1979

Calcif Tissue Int

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“…Type I procollagen molecules have been immunolocalized within autophagic lysosomal bodies in odontoblasts, suggesting the diversion of some collagen precursors from the secretory pathway into lysosomes (Karim et al, 1979). Cytochemical localization of acid phosphatase, along with other lysosomal enzymes, indicates that they might be released into predentin and that some portion of the proteoglycans and glycosaminoglycans might be broken down at the mineralization front of dentin (Nagai, 1970b;Linde and Persliden, 1977;Sasaki et al, 1982b). In this regard, Nygren et al (1979) localized the proteolytic enzyme, cathepsin D, in lysosomal and secretory vesicles of odontoblasts and in the predentin matrix.…”

Section: The Golgi Complex and Related Cell Organellesmentioning

confidence: 94%

Structure and organization of odontoblasts

Sasaki

Garant

1996

Anat. Rec.

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“…Loss of stems on the scale we describe does not appear to have been recorded, so there is little information on possible mechanisms. There are reports of a variety of enzymes produced by odontoblasts, or found nearby, e.g.. Linde and Persliden [1977]; Robinson and Birkedal-Hansen [ 1981 ] and Robinson et al [ 1984], Also. Sasaki et al [ 1984] have described endocytotic activity by odontoblasts.…”

Section: Discussionmentioning

confidence: 99%

Early Dentinogenesis in Mice: von Korff Fibres and Their Possible Significance

Wigglesworth¹,

Longmore²,

Kuc³

et al. 1986

Cells Tissues Organs

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By light and electron microscopy we have confirmed the collagenous nature of von Korff fibres in early dentinogenesis in mice. Each fibre array begins as an argyrophil ‘stem’ lying between the outermost cells of the dental papilla, with finer divisions passing into the papilla. With the appearance of odontoblasts, a cone-like ‘spray’ of non-argyrophil fibres in continuity with the stem spreads peripherally between these cells to the dental epithelial basement membrane. After the sprays become immured in dentine matrix, the stems are removed. Later, new stems appear. Sprays are restricted to enamel-bearing parts of teeth. The possible nature and functions of von Korff fibres are discussed.

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Cathepsind activity in isolated odontoblasts

Cited by 31 publications

References 33 publications

Cathepsin D: Ultra-immunohistochemical localization in dentinogenesis

Cathepsin D: Ultra-immunohistochemical localization in dentinogenesis

Structure and organization of odontoblasts

Early Dentinogenesis in Mice: von Korff Fibres and Their Possible Significance

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