Cathepsin K deficiency promotes alveolar bone regeneration by promoting jaw bone marrow mesenchymal stem cells proliferation and differentiation via glycolysis pathway

Zhang, Wuyang; Dong, Zhiwei; Li, Dengke; Li, Bei; Liu, Yuan; Zheng, Xueni; Liu, Hui; Zhou, Hongzhi; Hu, Kaijin; Xue, Yan

doi:10.1111/cpr.13058

Cited by 20 publications

(11 citation statements)

References 25 publications

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“…High expression of ALP activity is an early sign of osteoblast differentiation and maturation. Increased ALP activity enhances bone formation and promotes the formation of bone matrix mineralization [ 47 ]. As an essential transcription factor for osteoblast differentiation, RUNX2 encourages the expression of osteoblast secretion proteins, OCN and OPN [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Hsa_circ_0001485 promoted osteogenic differentiation by targeting BMPR2 to activate the TGFβ-BMP pathway

et al. 2022

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Background Circular RNAs (circRNAs) are a new type of stable noncoding RNA and have been proven to play a crucial role in osteoporosis. This study explored the role and mechanism of hsa_circ_0001485 in osteogenic differentiation. Methods Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and Gene Ontology (GO) enrichment analysis were performed according to the previous sequencing data in human bone marrow mesenchymal stem cells (BMSC) before and after the induction of osteogenic differentiation on the differentially expressed circRNAs, to screen out signaling pathways associated with osteogenic differentiation. The hFOB 1.19 cells were used to verify the function and mechanism of specific circRNAs in osteogenic differentiation. Additionally, small interfering fragments and overexpression plasmids were used to determine the role of specific circRNAs during osteogenic differentiation. Furthermore, pull-down experiments and mass spectrometry were performed to determine the proteins that bind to specific circRNAs. Results The KEGG and GO enrichment analyses showed that the TGFβ-BMP signaling pathway was related to the osteogenic differentiation process, and four circRNAs were associated with the pathway. The quantitative polymerase chain reaction analysis revealed that hsa_circ_0001485 expression was increased during the osteogenic differentiation process of BMSCs. Knockdown of hsa_circ_0001485 suppressed the activity of the alkaline phosphatase enzyme and the expression of RUNX2, osteopontin, and osteocalcin in the osteogenic hFOB 1.19 cells, whereas overexpression of hsa_circ_0001485 promoted their expression. Additionally, we found that hsa_circ_0001485 and BMPR2 targeted binding to activate the TGFβ-BMP signaling pathway and promoted osteogenic differentiation through mass spectrometry analysis. Conclusion This study demonstrates that hsa_circ_0001485 is highly expressed in the osteogenic hFOB 1.19 cells, which activate the TGFβ-BMP pathway through targeted binding of BMPR2, and plays a positive role in regulating osteogenic differentiation.

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Section: Discussionmentioning

confidence: 99%

Hsa_circ_0001485 promoted osteogenic differentiation by targeting BMPR2 to activate the TGFβ-BMP pathway

et al. 2022

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“…MSCs derive from oral alveolar bone and have multiple differentiation potentials. They can be differentiated into, e.g., osteoblasts, adipocytes, and endothelial cells, and contribute to repairing hard and soft tissue around the implant [ 15 ].Bone marrow mesenchymal stem cells are an important source of osteoblasts and are often used as core cells for repairing local bone, gristle, and myelogenous adipose tissue. These cells can be adhered to a titanium surface and activated in the osteogenesis and osseointegration phases [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

The effects of Twinlight laser treatment on the titanium surface proliferation and osteogenic differentiation of mesenchymal stem cells

et al. 2022

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Background The study aimed to observe the effects of a Twinlight laser on the titanium surface proliferation of inflammatory Mesenchymal stem cells (MSCs), inflammatory cytokine expression, and osteogenic differentiation. Methods The MSCs were collected from bone tissue of healthy individuals.The cellular inflammatory model was established with 1 μg/mL lipopolysaccharide (LPS).Under the cellular inflammatory model,divided into five groups: the normal control group (C); the inflammatory control group (L); Er:YAG laser group (L + E); Nd:YAG laser group (L + N); Er:YAG laser and Nd:YAG laser group (L + E + N). The treated cells were inoculated onto titanium disks.The normal and inflammatory MSCs on the surface of titanium surface were examined by CCK-8, scanning election microscopy (SEM), quantitative real-time polymerase chain reaction (qRT‑PCR) and other methods for their proliferation, growth pattern, expression of inflammatory factors Interleukin-6 (IL-6), Interleukin-8 (IL-8) and osteogenic genes Runx2 (Runt-related transcription factor 2) and alkaline phosphatase (ALP), providing the theoretical basis and experimental data for the Twinlight laser-assisted treatment of peri-implantitis. Statistical analyses were performed using a Student's t test with SPSS 17.0 software. Results Through observation using SEM, the cell densities of the L + E + N, L + E, and L + N groups were similar, but cell bodies in the L + E + N group were fuller and each had more than two pseudopodia. The expression level of IL-6 mRNA in the L, L + N, L + E, and L + E + N groups was higher than in group C (P < 0.05), and the expression level of IL-8 mRNA in the L + E + N group was significantly lower than in group L (P < 0.0001). On day 7, the expression level of ALP mRNA in the L, L + N, L + E, and L + E + N groups was lower than in group C (P < 0.05). On day 14, there was no significant difference in the expression level of ALP mRNA among the L + N, L + E + N, and C groups (P > 0.05). On day 7, the expression level of RUNX2 mRNA in the L + E + N group was higher than in group L (P < 0.001). On day 14, the expression level of RUNX2 mRNA in the L + E + N group was higher than in group L (P < 0.01). Conclusion Twinlight laser treatment promoted cell proliferation, inhibited the expression of inflammatory cytokines, and effectively enhanced the osteogenic differentiation of cells on a titanium surface.

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“…Despite various reports on tooth extraction socket healing ( Arioka et al, 2021 ; Horibe et al, 2021 ; Vieira et al, 2015 ; Wang et al, 2020 ; Yi et al, 2021 ; Yuan et al, 2018 ; Zhang et al, 2020 ; Zhang et al, 2021 ), the comparative studies on pathological changes in bone regeneration between the tooth socket and long bones have not been conducted. Tooth extraction socket healing has a unique environment during bone regeneration; it is always surrounded by alveolar bone, and this might provide constant stability and retention of plasma clots during the healing process.…”

Section: Introductionmentioning

confidence: 99%

“…For example, Debnath et al (2018) discovered that periosteal stem cells located in long bones and calvaria in mice could form bone by intramembranous ossification, whereas they acquired the capacity to undergo endochondral ossification depending on their plasticity. Recently, these techniques have been applied to determine the origin and fate of cells participating in tooth extraction socket healing ( Arioka et al, 2021 ; Wang et al, 2020 ; Yi et al, 2021 ; Yuan et al, 2018 ; Zhang et al, 2020 ; Zhang et al, 2021 ). Although most of these studies used mice that had been developed primary for the analysis of skeletal tissue development and regeneration other than dental tissues, application of these mice is valuable for disclosing the lineage and fate of cells involved in tooth socket healing.…”

Section: Introductionmentioning

confidence: 99%

Pathological differences in the bone healing processes between tooth extraction socket and femoral fracture

et al. 2022

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Cathepsin K deficiency promotes alveolar bone regeneration by promoting jaw bone marrow mesenchymal stem cells proliferation and differentiation via glycolysis pathway

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 20 publications

References 25 publications

Hsa_circ_0001485 promoted osteogenic differentiation by targeting BMPR2 to activate the TGFβ-BMP pathway

Hsa_circ_0001485 promoted osteogenic differentiation by targeting BMPR2 to activate the TGFβ-BMP pathway

The effects of Twinlight laser treatment on the titanium surface proliferation and osteogenic differentiation of mesenchymal stem cells

Pathological differences in the bone healing processes between tooth extraction socket and femoral fracture

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