Cathepsin H and cathepsin B of Cynoglossus semilaevis are involved in anti-bacterial immunity against Edwardsiella tarda

Wang, Yilong; Xu, Hang; Sun, Bo

doi:10.1016/j.fsi.2023.108594

Cited by 5 publications

(2 citation statements)

References 46 publications

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“…The potential mechanism by which cathepsin H reduces the risk of IPF is not clear. A recent study has highlighted the role of cathepsin H in antimicrobial immunity (39). In addition, a research team reported that knocking out cathepsin H in a mouse model of experimental autoimmune encephalomyelitis resulted in increased Th1 cell differentiation and early onset of autoimmune encephalomyelitis (40).…”

Section: Discussionmentioning

confidence: 99%

Causality between cathepsins and idiopathic pulmonary fibrosis: a Mendelian randomization study

Zhong,

Xu,

et al. 2024

Preprint

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Background The pathogenesis of idiopathic pulmonary fibrosis (IPF) is complex and difficult to diagnose and treat clinically. Cathepsins are involved in various physiological and pathological processes, and observational studies have shown an association between cathepsins and IPF. However, the causal relationship between them remains uncertain. Our aim was to assess the causal relationship between various cathepsins and IPF. Methods Protein quantitative trait loci (pQTL) data for cathepsins were obtained from INTERVAL studies, and summary statistics for IPF genome-wide association studies (GWAS) were obtained from the FinnGen R10 study. Univariable Mendelian randomization (UVMR), multivariable Mendelian randomization (MVMR), summary data-based Mendelian randomization (SMR) and Bayesian colocalization analysis were conducted in this study. Results UVMR analysis indicated that elevated cathepsin H levels reduced the overall risk of IPF (OR = 0.885,95%CI = 0.827 ~ 0.947, P = 3.86×10 − 4). MVMR showed that the effect of cathepsin H on IPF was still present after adjusting the interaction of cathepsins (OR = 0.895,95%CI = 0.834 ~ 0.961, P = 0.002). In addition, SMR analysis also suggested a causal association between cathepsin H and IPF (OR = 0.800,95%CI = 0.699 ~ 0.916, P = 0.001). Finally, we validated the results using the UK Biobank Pharma Proteomics Project (UKB-PPP) dataset (OR = 0.897,95%CI = 0.836 ~ 0.963, P = 0.003). Conclusions This study suggests that cathepsin H has a protective effect on IPF and may serve as a potential therapeutic target for IPF, providing inspiration and guidance for the diagnosis and treatment of IPF.

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Section: Discussionmentioning

confidence: 99%

Causality between cathepsins and idiopathic pulmonary fibrosis: a Mendelian randomization study

Zhong,

Xu,

et al. 2024

Preprint

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“…Moreover, a notable increase in protein expression was observed for five cathepsin proteins (cathepsin D, X, B, L, S) following immersion in DEL. Cathepsins play a crucial role in multiple immune processes, including pathogen recognition and elimination, antigen processing and presentation, and apoptosis [ 59 ]. Additionally, as core mucus components, proteases have the ability to augment the synthesis of other innate immune components, such as complement systems and immunoglobulins [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

Conventional Anthelmintic Concentration of Deltamethrin Immersion Disorder in the Gill Immune Responses of Crucian Carp

Wu,

Yuan,

Gao

et al. 2023

Toxics

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Current treatment strategies for parasitic infectious diseases in crucian carp primarily rely on chemotherapy. As a commonly used antiparasitic agent, deltamethrin (DEL) may have the potential adverse effects on external mucosa of fish such as gills. In this study, 180 healthy juvenile crucian carp (Carassius auratus) (average weight: 8.8 ± 1.0 g) were randomly divided into three groups for 28 days, which were immersed in 0 μg/L, 0.3 μg/L, and 0.6 μg/L of DEL, respectively. The results of histological analysis revealed that severe hyperplasia in the secondary lamellae of gills was observed, and the number of goblet (mucus-secreting) cells increased significantly after DEL immersion. TUNEL staining indicated that the number of apoptotic cells increased in crucian carp gill. At the molecular level, the mRNA expression analysis revealed significant upregulation of apoptosis (caspase 3, caspase 8, and bax), autophagy (atg5 and beclin-1), and immune response (lzm, muc5, il-6, il-8, il-10, tnfα, ifnγ, tgfβ, tlr4, myd88, and nf-kb), whereas tight junction-related genes (occludin and claudin12) were downregulated after DEL immersion, suggesting that DEL immersion altered innate immunity responses and promoted mucus secretion. Moreover, tandem mass tag (TMT)-based proteomics revealed that a total of 428 differentially expressed proteins (DEPs) contained 341 upregulated DEPs and 87 downregulated DEPs with function annotation were identified between the control and DEL groups. Functional analyses revealed that the DEPs were enriched in apoptotic process, phagosome, and lysosome pathways. Additionally, DEL immersion also drove gill microbiota to dysbiosis and an increase in potentially harmful bacteria such as Flavobacterium. Overall, this study showed that DEL elicited shifts in the immune response and changes in the surface microbiota of fish. These results provide new perspectives on the conventional anthelmintic concentration of DEL immersion disorder of the gill immune microenvironment in crucian carp and theoretical support for future optimization of their practical application.

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Functional insights of digestion, absorption, and immunity in different segments of the intestine in Hemibarbus labeo from transcriptomic analysis

Lv,

Gao,

Dai

et al. 2024

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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Cathepsin H and cathepsin B of Cynoglossus semilaevis are involved in anti-bacterial immunity against Edwardsiella tarda

Cited by 5 publications

References 46 publications

Causality between cathepsins and idiopathic pulmonary fibrosis: a Mendelian randomization study

Causality between cathepsins and idiopathic pulmonary fibrosis: a Mendelian randomization study

Conventional Anthelmintic Concentration of Deltamethrin Immersion Disorder in the Gill Immune Responses of Crucian Carp

Functional insights of digestion, absorption, and immunity in different segments of the intestine in Hemibarbus labeo from transcriptomic analysis

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