Cathepsin D from Atlantic cod (Gadus morhua L.) liver. Isolation and comparative studies

Molecular identification and expression analysis of cathepsins O and S from rock bream, Oplegnathus fasciatus

“…However, using antibodies directed against chum salmon cathepsins B and L, it was reported that these enzymes were derived from muscle-invading phagocytes [36]. Antibodies directed against proteolytic enzymes may also be useful tools for understanding the roles of these enzymes in post-mortem muscle degradation [37]. To verify the functions of cathepsin O and S related to post-mortem muscle degradation in the rock bream, further investigations involving construction of recombinant proteins and enzyme activity assays are required.…”

Occurrence of aspartyl proteases in brine after herring marinating

“…Cathepsin D isolated from herring, mackerel and cod exhibits the highest activity against haemoglobin at pH 2.5, 3.0 and 4.0, respectively (Jiang, Her, Lee, & Wang, 1993;McLay, 1980;Nielsen & Nielsen, 2001;Wang, Stenvik, Larsen, Maehre, & Olsen, 2007). Komai, Kawabata, Amano, Lee, and Ichishima (2004) demonstrated that the optimum pH of cathepsin D activity against synthetic substrate was lower by 0.5 units than against haemoglobin.…”

Molecular characterization and expression analysis of the channel catfish cathepsin D genes

“…In most fish species, cathepsin D was found to be mainly expressed in the spleen and liver [19], but it is also abundant in skin mucosa [21] and muscle based on protein levels [11,17,22]. Because of its major functions in protein degradation in the lysosomes and because it produces parasin I, an antimicrobial peptide, it is believed that cathepsins are important for innate immunity [21].…”

“…Cathepsin D has been cloned and sequenced in a number of mammalian species [9,10], and in several species of fish such as tilapia (Tilapia nilotica  Tilapia aurea) [11], rainbow trout (Oncorhynchus mykiss) [12], Antarctic icefish (Chionodraco hamatus) [13], seabream (Sparus aurata) [14], zebrafish (Danio rerio) [15], carp (Cyprinus carpio) [16], herring (Clupea harengus) [17], pufferfish (Takifugu rubripes) [18], Atlantic cod (Gadus morhua) [19], and turbot (Scophthalmus maximus) [20]. In most fish species, cathepsin D was found to be mainly expressed in the spleen and liver [19], but it is also abundant in skin mucosa [21] and muscle based on protein levels [11,17,22].…”