Cathelicidin protects against Helicobacter pylori colonization and the associated gastritis in mice

Zhang, Lin; Yu, Jun; Wong, Corine Sau Man; Ling, T. K. W.; Li, Z J; Chan, Kam Ming; Ren, Shun X.; Shen, Jianfa; Chan, Ruby Lok Yi; Lee, C C; Li, M S M; Cheng, Alfred S.L.; To, Ka‐Fai; Gallo, Richard L.; Sung, Joseph J.Y.; Wu, William Ka Kei; Cho, C H

doi:10.1038/gt.2012.92

Cited by 40 publications

(32 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Paraformaldehyde-fixed stomach tissues were embedded in paraffin, sectioned at 5 mm, and then stained with hematoxylin and eosin as previously described (Zhang et al, 2013).…”

Section: Stomach Histologymentioning

confidence: 99%

Changes in gastric microbiota induced by Helicobacter pylori infection and preventive effects of Lactobacillus plantarum ZDY 2013 against such infection

Pan

Wan

Xie

et al. 2016

Journal of Dairy Science

View full text Add to dashboard Cite

Helicobacter pylori is a gram-negative pathogen linked to gastric ulcers and stomach cancer. Gastric microbiota might play an essential role in the pathogenesis of these stomach diseases. In this study, we investigated the preventive effect of a probiotic candidate Lactobacillus plantarum ZDY 2013 as a protective agent against the gastric mucosal inflammation and alteration of gastric microbiota induced by H. pylori infection in a mouse model. Prior to infection, mice were pretreated with or without 400 µL of L. plantarum ZDY 2013 at a concentration of 10(9) cfu/mL per mouse. At 6 wk postinfection, gastric mucosal immune response and alteration in gastric microbiota mice were examined by quantitative real-time PCR and high-throughput 16S rRNA gene amplicon sequencing, respectively. The results showed that L. plantarum ZDY 2013 pretreatment prevented increase in inflammatory cytokines (e.g., IL-1β and IFN-γ) and inflammatory cell infiltration in gastric lamina propria induced by H. pylori infection. Weighted UniFrac principal coordinate analysis showed that L. plantarum ZDY 2013 pretreatment prevented the alteration in gastric microbiota post-H. pylori infection. Linear discriminant analysis coupled with effect size identified 22 bacterial taxa (e.g., Pasteurellaceae, Erysipelotrichaceae, Halomonadaceae, Helicobacteraceae, and Spirochaetaceae) that overgrew in the gastric microbiota of H. pylori-infected mice, and most of them belonged to the Proteobacteria phylum. Lactobacillus plantarum ZDY 2013 pretreatment prevented this alteration; only 6 taxa (e.g., Lachnospiraceae, Ruminococcaceae, and Clostridiaceae), mainly from the taxa of Firmicutes and Bacteroidetes, were dominant in the gastric microbiota of the L. plantarum ZDY 2013 pretreated mice. Administration of L. plantarum ZDY 2013 for 3 wk led to increase in several bacterial taxa (e.g., Rikenella, Staphylococcus, Bifidobacterium), although a nonsignificant alteration was found in the gastric microbiota. Overall, this study demonstrated that L. plantarum ZDY 2013 pretreatment played an important role in preventing gastric mucosal inflammation and gastric microbiota alteration induced by H. pylori infection, and the selective modulation in gastric microbiota posed by this intervention suggested that targeting gastric microbiota through oral administration of probiotics might be an alternative strategy to prevent H. pylori infection.

show abstract

“…Paraformaldehyde-fixed stomach tissues were embedded in paraffin, sectioned at 5 mm, and then stained with hematoxylin and eosin as previously described (Zhang et al, 2013).…”

Section: Stomach Histologymentioning

confidence: 99%

Changes in gastric microbiota induced by Helicobacter pylori infection and preventive effects of Lactobacillus plantarum ZDY 2013 against such infection

Pan

Wan

Xie

et al. 2016

Journal of Dairy Science

View full text Add to dashboard Cite

show abstract

“…The in vitro analysis of LL37 expression in cultured gastric cells showed that the increase in expression by H. pylori was dependent on a functional type 4 secretion system (T4SS) encoded by the cag pathogenicity island (cagPAI) of H. pylori. The importance of cathelicidin in the control of H. pylori infection was demonstrated using mice deficient in the murine LL37 ortholog (Zhang et al, 2013). Knockout mice displayed higher bacterial load in the stomach and increased H. pylori-associated gastritis.…”

Section: Introductionmentioning

confidence: 99%

Survival in hostile territory: the microbiota of the stomach

2013

View full text Add to dashboard Cite

The human stomach is a formidable barrier to orally ingested microorganisms and was long thought to be sterile. The discovery of Helicobacter pylori, a carcinogenic bacterial pathogen that infects the stomach mucosa of more than one half of all humans globally, has started a major paradigm shift in our understanding of the stomach as an ecological niche for bacteria. The special adaptations that enable H. pylori to colonize this well-protected habitat have been intensively studied over the last three decades. In contrast, our knowledge concerning bacteria other than H. pylori in the human stomach is still quite limited. However, a substantial body of evidence documents convincingly that bacteria can regularly be sampled from the stomachs of healthy adults. Commonly detected phyla include Firmicutes, Actinobacteria, Bacteroidetes, and Proteobacteria, and characteristic genera are Lactobacillus, Streptococcus, and Propionibacterium. In this review, we summarize the available literature about the gastric microbiota in humans and selected model animals, discuss the methods used in its characterization, and identify gaps in our knowledge that need to be addressed to advance our understanding of the bacterial colonization of the different layers of the gastric mucosa and its potential role in health and disease.

show abstract

“…Cathelicidins and defensins are examples of human antimicrobial peptides (AMPs) native to the innate immune system of many eukaryotes that have activity against H. pylori [150] . LL-37 is a cathelicidin with an anti-H. pylori effect [151] , and a recent study demonstrated that cathelicidin limited H. pylori colonization and related gastritis in mouse models [152] . Defensin peptides have also been indicated to impede H. pylori [153] .…”

Section: Eradication: Hope Is Coming?mentioning

confidence: 99%

Antibiotic treatment forHelicobacter pylori: Is the end coming?

Kim

Choi

Chung

2015

WJGPT

View full text Add to dashboard Cite

Infection with the Gram-negative pathogen Helicobacter pylori (H. pylori ) has been associated with gastroduodenal disease and the importance of H. pylori eradication is underscored by its designation as a group I carcinogen. The standard triple therapy consists of a proton pump inhibitor, amoxicillin and clarithromycin, although many other regimens are used, including quadruple, sequential and concomitant therapy regimens supplemented with metronidazole, clarithromycin and levofloxacin. Despite these efforts, current therapeutic regimens lack efficacy in eradication due to antibiotic resistance, drug compliance and antibiotic degradation by the acidic stomach environment. Core tip: This article reviews the recent literature describing antibiotic resistance and trends in Helicobacter pylori (H. pylori ) treatment. As there is no effective conventional therapy, new treatments are being developed and bismuth quadruple, sequential, concomitant therapies are recommended as a first-line regimen in regions with high levels of clarithromycin resistance. Quinolones have also been used for H. pylori treatment, although the cure rate has gradually reduced with this approach. New therapeutic directions include probiotic supplementation, tailored therapy, novel agents, and nanotechnology.

show abstract

Cathelicidin protects against Helicobacter pylori colonization and the associated gastritis in mice

Cited by 40 publications

References 44 publications

Changes in gastric microbiota induced by Helicobacter pylori infection and preventive effects of Lactobacillus plantarum ZDY 2013 against such infection

Changes in gastric microbiota induced by Helicobacter pylori infection and preventive effects of Lactobacillus plantarum ZDY 2013 against such infection

Survival in hostile territory: the microbiota of the stomach

Antibiotic treatment forHelicobacter pylori: Is the end coming?

Contact Info

Product

Resources

About