Catestatin is involved in neuropathic pain mediated by purinergic receptor P2X4 in the spinal microglia of rats

Deng, Zhi-Xiong; Li, Congcong; Liu, Chenglong; Du, Errong; Xu, Changshui

doi:10.1016/j.brainresbull.2018.07.003

Cited by 13 publications

(10 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The purinergic receptors P2X4 and P2X7 are overexpressed on the cell surface of spinal dorsal horn microglia involved in microglial activation, which significantly contributes to the inflammation after SCI ( Deng et al, 2018 ; Du et al, 2019 ; Kobayakawa et al, 2019 ; Song et al, 2022 ). Electroacupuncture can inhibit P2X7 receptor-mediated microglial activation and attenuate neuropathic pain ( Wu et al, 2021a ).…”

Section: Mechanism Of Acupuncture Therapy In Spinal Cord Injurymentioning

confidence: 99%

Mechanism Underlying Acupuncture Therapy in Spinal Cord Injury: A Narrative Overview of Preclinical Studies

Jiang¹,

Sun²,

Chen³

2022

Front. Pharmacol.

View full text Add to dashboard Cite

Spinal cord injury (SCI) results from various pathogenic factors that destroy the normal structure and function of the spinal cord, subsequently causing sensory, motor, and autonomic nerve dysfunction. SCI is one of the most common causes of disability and death globally. It leads to severe physical and mental injury to patients and causes a substantial economic burden on families and the society. The pathological changes and underlying mechanisms within SCI involve oxidative stress, apoptosis, inflammation, etc. As a traditional therapy, acupuncture has a positive effect promoting the recovery of SCI. Acupuncture-induced neuroprotection includes several mechanisms such as reducing oxidative stress, inhibiting the inflammatory response and neuronal apoptosis, alleviating glial scar formation, promoting neural stem cell differentiation, and improving microcirculation within the injured area. Therefore, the recent studies exploring the mechanism of acupuncture therapy in SCI will help provide a theoretical basis for applying acupuncture and seeking a better treatment target and acupuncture approach for SCI patients.

show abstract

Section: Mechanism Of Acupuncture Therapy In Spinal Cord Injurymentioning

confidence: 99%

Mechanism Underlying Acupuncture Therapy in Spinal Cord Injury: A Narrative Overview of Preclinical Studies

Jiang¹,

Sun²,

Chen³

2022

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…P2X is dependent on the ATP ion channel receptor and plays a key role in the development of nerve injury and pain ( Kato et al, 2017 ). P2 × 4 receptors are important members of the P2X family and play a key role in the development of pain ( Xiaodi et al, 2010 ; Deng et al, 2018 ). After the nervous system is subjected to noxious stimuli, ATP is released in large quantities, activates the P2 × 4 receptors, enhances sensory information transmission, sensitizes the central nervous system, and induces pain.…”

Section: Discussionmentioning

confidence: 99%

“…Each stimulation time is not less than 15 s. When the right hind limb of the rats was stimulated, the feet of the rats were lifted and retracted, and the minimum stimulation intensity was regarded as the MWT. This was repeated three times, and the average values were taken ( Deng et al, 2018 ).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Microencapsulated Neural Stem Cells Inhibit Sciatic Nerve Injury-Induced Pain by Reducing P2 × 4 Receptor Expression

Zhang

Luo

Huang

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Objectives: The purpose of this study is to investigate the effects of transplantation of microencapsulated neural stem cells (MC-NSCs), which downregulate the P2 × 4 receptor (P2 × 4R) overexpression and relieve neuropathic pain (NPP).Methods: Neural stem cells (NSCs) and MC-NSCs were transplanted to the injured sciatic nerve. Transmission electron microscope and immunofluorescence were used to observe the changes of injured sciatic nerve. Behavioral methods were used to detect mechanical withdrawal thresholds (MWT) and thermal withdrawal latency (TWL) of rats. Expression levels of P2 × 4Rs and p-p65 in the spinal cord segment of rats were measured by using molecular biology methods. The concentrations of IL-1β and TNF-α were detected in serum of rats by ELISA.Results: After sciatic nerve injury, the sciatic nerve fibers had the myelinated lamina separated, and disintegrated fragments could be seen. The fluorescence intensity of myelin MBP was weakened. The MWT and TWL were significantly decreased, the expression of P2 × 4Rs and p-p65 were significantly increased, and the concentrations of IL-1β and TNF-α were increased. After NSC and MC-NSC transplantation, the myelin sheath of the sciatic nerve was relatively intact, some demyelination changes could be seen, and the injured sciatic nerve has been improved. The fluorescence intensity of myelin MBP was increased. The MWT and TWL were increased, expression levels of P2 × 4Rs and p-p65 were decreased, and the concentrations of IL-1β and TNF-α were significantly decreased. Compared with NSC transplantation, transplantation of MC-NSCs could better repair the damaged sciatic nerve, decrease the expression of P2 × 4Rs and p-p65, decrease the level of IL-1β and TNF-α, and relieve pain (all p-values < 0.05).Conclusion: NSCs and MC-NSCs transplantation may alleviate pain by reducing the expression of P2 × 4Rs and inhibiting the activation of NF-KB signaling, while MC-NSCs transplantation has a better effect of suppressing pain. Our experimental results provide new data support for the treatment of NPP.

show abstract

“…An electromechanical pain meter (BIO-EVF4, France) was used to test the MWT. The TWL measurement was conducted as the following procedures ( Deng et al, 2018 ). The TWL was detected by a thermal radiation simulator (BME-390, United States).…”

Section: Methodsmentioning

confidence: 99%

Effect of Bufalin-PLGA Microspheres in the Alleviation of Neuropathic Pain via the CCI Model

et al. 2022

View full text Add to dashboard Cite

The treatment of neuropathic pain (NPP) is considered challenging, while the search for alternative medication is striving. NPP pathology is related with the expression of both the purinergic 2X7 (P2X7) receptor and the transient receptor potential vanilloid 1 receptor (TRPV1). Bufalin is a traditional Chinese medication derived from toad venom with pronounced antitumor, analgesic, and anti-inflammatory properties. However, poor solubility, rapid metabolism, and the knowledge gap on its pain alleviation mechanism have limited the clinical application of bufalin. Hence, the purpose of this study is to illustrate the NPP alleviation mechanism of bufalin via chronic constriction injury (CCI). To address the concern on fast metabolism, bufalin-PLGA microspheres (MS) were prepared via membrane emulsification to achieve prolonged pain-relieving effects. Western blot, real-time PCR, immunofluorescence, and molecular docking were employed to demonstrate the therapeutic action of bufalin on NPP. The results showed enhanced thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) after the administration of both bufalin and bufalin-PLGA MS in the CCI rats. Prolonged pain-relieving effects for up to 3 days with reduced dose frequency was achieved via bufalin-PLGA MS. In the CCI rats treated with bufalin-PLGA MS, the expression levels of protein and mRNA in TRPV1 and P2X7, both localized in the dorsal root ganglion (DRG), were reduced. Moreover, bufalin-PLGA MS effectively reduced the levels of IL-1β, IL-18, IL-6, and TNF-α in the CCI group. The results from molecular docking suggested a possible mechanism of NPP alleviation of bufalin through binding to P2X7 receptors directly. The administration of bufalin-PLGA MS prepared by membrane emulsification demonstrated promising applications for sustained effect on the alleviation of NPP.

show abstract

Catestatin is involved in neuropathic pain mediated by purinergic receptor P2X4 in the spinal microglia of rats

Cited by 13 publications

References 45 publications

Mechanism Underlying Acupuncture Therapy in Spinal Cord Injury: A Narrative Overview of Preclinical Studies

Mechanism Underlying Acupuncture Therapy in Spinal Cord Injury: A Narrative Overview of Preclinical Studies

Microencapsulated Neural Stem Cells Inhibit Sciatic Nerve Injury-Induced Pain by Reducing P2 × 4 Receptor Expression

Effect of Bufalin-PLGA Microspheres in the Alleviation of Neuropathic Pain via the CCI Model

Contact Info

Product

Resources

About