Catecholamine-Induced Stimulation of Progesterone by the Bovine Corpus Luteum in vitro1

Influence of adrenergic receptors on ovarian progesterone secretion in the pseudopregnant cat and oestradiol secretion in the oestrous cat

“…These observations extend earlier findings that adrenergic stimulation increases progesterone concentrations produced by the human (Fylling, 1971) and sheep (Flint et secretion in vitro by bovine (Condon & Black, 1976), ovine (Jordan et ai, 1978) and rat (Ratner et ai, 1980) luteal tissue and human placental tissue (Caritis et al, 1983), and rat ovarian secretion (Zsolnai et ai, 1982 There is evidence that stress, and presumably increased sympathetic tone (Selye, 1950), stimulates increased progesterone concentrations. Undernutrition of the sheep results in greater peripheral progesterone concentrations (Cumming et al, 1971).…”

“…It has been demonstrated that stimulating or inhibiting \g=b\-adrenergic neurotransmitter receptors affects progesterone steroidogenesis in terms of ovarian tissue content or secretion in vitro (cow: Condon & Black, 1976; sheep: Jordan et al, 1978;rat: Ratner et al, 1980) and peripheral blood concentrations (human : Fylling, 1971; sheep: Flint et al, 1974). However, some experiments measuring blood progesterone concentrations in pregnant women (Carlstr\l=o"\m et al, 1978;Hanssens et al, 1983) and luteal tissue secretion in vitro (Richardson & Mason, 1980) have yielded contradictory results.…”

Differential regulation by LH and prostaglandins of steroidogenesis in small and large luteal cells of the ewe

“…The corpora lutea were dissociated into single-cell suspensions using collagenase, and the number of cells recovered from each corpus luteum was determined with a haemocytometer. The cells were fixed in 1% paraformaldehyde, washed, dried onto slides, and histochemically stained for 3ß-hydroxysteroid dehydrogenase (3ß-HSD) activity, as described by Payne, Downing & Wong (1980 (Archbald, Al-Bagdadi & Godke, 1981 (Condon & Black, 1976), but the effects on purified populations of small and large cells have not been examined. Receptors for oestradiol are more abundant in large luteal cells than in small luteal cells (Glass, Fitz & Niswender, 1984), but their function remains undetermined.…”

Concentrations of catecholamines, ascorbic acid, progesterone and oxytocin in the corpora lutea of cyclic and pregnant cattle

“…and in vitro [3,5]. Moreover, noradrenaline (NA) is synthesised in bovine CL from its precursor dopamine (DA) [19].…”