Catechol 2,3‐dioxygenase from a new phenolic compound degrader <i>Thauera</i> sp. K11: purification and biochemical characterization

Xi, Lijun; Liu, Dejian; Wang, Lingling; Qiao, Nenghu; Liu, Jianguo

doi:10.1002/jobm.201700566

Cited by 20 publications

(3 citation statements)

References 32 publications

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“…C23O-K11 is from Thauera sp. K11 54 ; C23O1-Hb and C23O2-Hb are from a hallophilic bacterial consortium 47 ; C23O-P5 is from Planococcus sp.S542 41 ; C23O-Sm is from Stenotrophomonas maltophilia KB243 53 ; C23O-P47 is from Pseudomonas sp. S-4748 80 ; C23O-Pp is from Pseudomonas putida mt-2 81 ; C23O-Ps is cell extract from o -xylene grown Pseudomonas stutzeri strain 82 .…”

Section: Resultsmentioning

confidence: 99%

“…For example, different buffers and concentrations of metals used by different groups may both affect the results. Published literature used Tris buffer 52 or phosphate buffer 28 , 47 , 53 and metal concentrations ranged from 1–3 μM 53 , 0.01 mM 28 to 5 mM 47 , 54 . We used the intermediate concentrations of 0.1 and 1 mM for each metal and found some inconsistencies.…”

Section: Resultsmentioning

confidence: 99%

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A novel Bacillus ligniniphilus catechol 2,3-dioxygenase shows unique substrate preference and metal requirement

Adewale

Lang

Huang

et al. 2021

Sci Rep

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Identification of novel enzymes from lignin degrading microorganisms will help to develop biotechnologies for biomass valorization and aromatic hydrocarbons degradation. Bacillus ligniniphilus L1 grows with alkaline lignin as the single carbon source and is a great candidate for ligninolytic enzyme identification. The first dioxygenase from strain L1 was heterologously expressed, purified, and characterized with an optimal temperature and pH of 32.5 °C and 7.4, respectively. It showed the highest activity with 3-ethylcatechol and significant activities with other substrates in the decreasing order of 3-ethylcatechol > 3-methylcatechol > 3-isopropyl catechol > 2, 3-dihydroxybiphenyl > 4-methylcatechol > catechol. It did not show activities against other tested substrates with similar structures. Most reported catechol 2,3-dioxygenases (C23Os) are Fe2+-dependent whereas Bacillus ligniniphilus catechol 2,3-dioxygenase (BLC23O) is more Mn2+- dependent. At 1 mM, Mn2+ led to 230-fold activity increase and Fe2+ led to 22-fold increase. Sequence comparison and phylogenetic analyses suggested that BL23O is different from other Mn-dependent enzymes and uniquely grouped with an uncharacterized vicinal oxygen chelate (VOC) family protein from Paenibacillus apiaries. Gel filtration analysis showed that BLC23O is a monomer under native condition. This is the first report of a C23O from Bacillus ligniniphilus L1 with unique substrate preference, metal-dependency, and monomeric structure.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

A novel Bacillus ligniniphilus catechol 2,3-dioxygenase shows unique substrate preference and metal requirement

Adewale

Lang

Huang

et al. 2021

Sci Rep

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“…Structures of various groups of phenolic compounds (Hussain, 2016; Mandal et al, 2017; Xi et al, 2018). …”

Section: Introductionmentioning

confidence: 99%

Role of Natural Phenolics in Hepatoprotection: A Mechanistic Review and Analysis of Regulatory Network of Associated Genes

et al. 2019

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The liver is not only involved in metabolism and detoxification, but also participate in innate immune function and thus exposed to frequent target Thus, they are the frequent target of physical injury. Interestingly, liver has the unique ability to regenerate and completely recoup from most acute, non-iterative situation. However, multiple conditions, including viral hepatitis, non-alcoholic fatty liver disease, long term alcohol abuse and chronic use of medications can cause persistent injury in which regenerative capacity eventually becomes dysfunctional resulting in hepatic scaring and cirrhosis. Despite the recent therapeutic advances and significant development of modern medicine, hepatic diseases remain a health problem worldwide. Thus, the search for the new therapeutic agents to treat liver disease is still in demand. Many synthetic drugs have been demonstrated to be strong radical scavengers, but they are also carcinogenic and cause liver damage. Present day various hepatic problems are encountered with number of synthetic and plant based drugs. Nexavar (sorafenib) is a chemotherapeutic medication used to treat advanced renal cell carcinoma associated with several side effects. There are a few effective varieties of herbal preparation like Liv-52, silymarin and Stronger neomin phages (SNMC) against hepatic complications. Plants are the huge repository of bioactive secondary metabolites viz; phenol, flavonoid, alkaloid etc. In this review we will try to present exclusive study on phenolics with its mode of action mitigating liver associated complications. And also its future prospects as new drug lead.

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Acclimation of activated sludge to nonylphenol ethoxylates and mathematical modeling of the depolymerization process

Arturi

Zaritzky

Contreras

2022

Braz. J. Chem. Eng.

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Catechol 2,3‐dioxygenase from a new phenolic compound degrader Thauera sp. K11: purification and biochemical characterization

Cited by 20 publications

References 32 publications

A novel Bacillus ligniniphilus catechol 2,3-dioxygenase shows unique substrate preference and metal requirement

A novel Bacillus ligniniphilus catechol 2,3-dioxygenase shows unique substrate preference and metal requirement

Role of Natural Phenolics in Hepatoprotection: A Mechanistic Review and Analysis of Regulatory Network of Associated Genes

Acclimation of activated sludge to nonylphenol ethoxylates and mathematical modeling of the depolymerization process

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