Catechol 1,2-dioxygenase from the new aromatic compounds – Degrading Pseudomonas putida strain N6

Effective biochemical decomposition of chlorinated aromatic hydrocarbons with a biocatalyst immobilized on a natural enzyme support

“…From the SDS-PAGE analysis (Fig. 2a), the enzyme was found to be 35 kDa in size, which was similar to the size of catechol dioxygenase reported in previous studies (average size range of 30-35 kDa, Caposio et al, 2002;Guzik et al, 2011;Lang et al, 2005). The greatest enzyme expression in the soluble form was obtained at an IPTG concentration of 0.3 mM at 15°C for 24 h (Fig.…”

“…The specific activity of cphA-I enzyme for catechol was 0.406 U/mg protein, which is 400-600,000 times greater than those reported in previous studies: Guzik et al (2011) and Nordin et al (2005) obtained 0.96 mU/mg protein and 0.67 lU/mg protein of catechol dioxygenase activity, respectively. This remarkable discrepancy was attributed to the superior purity of our enzyme as compared to those of the enzymes of the previous studies, which were obtained from crude extracts of the enzyme isolates.…”

“…2b and c indicate that a large amount of the purified enzyme was obtained through this two-step purification process. Catechol dioxygenase enzymes have been extracted from various strains capable of degrading phenolic compounds in a number of previous studies (Fernandez-Lafuente et al, 2000;Guzik et al, 2011;Jencova et al, 2004;Kim et al, 2011). However, it should be noted that these enzymes were isolated in the crude extract form.…”

Biodegradation of Benzidibe by Alkaliphilic Strain Bacillus badius D

“…It is well reputed that effect of particular concentration, temperature, pH, carbon and nitrogen affect significantly on biodegradation [42][43][44][45]. The biotransformation enzyme induction was reported in case of ‫ﻻ‬ Proteobacterium and others [46][47][48][49].…”

Degradation of monoaromatics by Bacillus pumilus MVSV3

“…This finding was similar to the enzyme isolated by Kou and Li 22 who reported the C12D isolated from Pseudomonas putida 84103 to exhibit highest activity at the optimum pH 7.5-8.0. Also, on the other hand, C12D isolated from Psuedomonas putida strain N6 showed highest activity at pH 7.4 23 . The enzyme isolated from Bacillus pumilus lost about 90% of its enzyme activity at pH below 5.0 and 80% was lost at pH 9.0.…”