Catch and release strategy of matrix metalloprotease aptamers via thiol–disulfide exchange reaction on a graphene based electrochemical sensor

Abstract

Matrix metalloproteases (MMP) are a large family of proteases which synergistically degrade abundant extracellular matrix and play an essential role in tissue degradation and remodelling. Sensing of MMP levels, notably...

Advances in electrochemical sensors for real-time glucose monitoring

“…In recent years, there has been a burgeoning interest in leveraging nanomaterials to pioneer the development of cutting-edge electrochemical sensors for glucose monitoring in sweat. 24,32,47–49 This surge in research activity has resulted in the exploration of a diverse array of nanomaterials, ranging from carbon-based materials 50 such as graphene 51,52 and carbon nanotubes 53,54 to metal-based nanoparticles like gold 47,55 and silver. 56,57 These nanomaterials possess unique properties such as high surface area, exceptional conductivity, and catalytic activity, rendering them highly suitable for enhancing the performance of glucose sensors.…”

Electrochemical Detection of MMP-2 Using Graphene-Based Aptasensor

“…Mishyn and co-workers developed an MMP-2 aptasensor with gold interdigitated electrodes (IDE) functionalized with CVD graphene, which was modified with an anti-MMP-2 aptamer. By using differential pulse voltammetry (DPV), a limit of detection of 100 pM (920 pg mL −1 ) was obtained [5].…”

Simultaneous quantification of exosomal MMP14 expression and proteolysis activity on a spherical dual-probe-based fluorescent nanosensor

“…Owing to their simplicity, high efficiency, and high sensitivity, biosensors based on different materials and probes have attracted much more attention in recent years. Activity based probes, 9,[16][17][18][19] substrate-based probes, [20][21][22] antibody probes, 9,[23][24][25] and aptamer probes [26][27][28] are commonly used in MMP biosensors. Peptide-and substrate-based sensors only react with proteolytically active MMPs, while labelled antibodyor aptamer-based sensors are used to detect the abundance of MMPs.…”