Cataract Formation through the Polyol Pathway is associated with Free Radical Production

Kubo, Eri; Miyoshi, Norio; Fukuda, Masaru; Akagi, Yoshio

doi:10.1006/exer.1998.0624

Cited by 67 publications

(43 citation statements)

References 18 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…High glucose levels attenuate the function of LECs through the generation of free radicals [13,16,29], suggesting a possible pathophysiological linkage between the high level of glucose and balance of anti-oxidants. Our study revealed decreased expression of AOP2 mRNA and protein in the lenses of rats with diabetes-or galactose-induced cataracts.…”

Section: Discussionmentioning

confidence: 99%

“…To induce sugar cataract, ten rats were given free access to 50% D-galactose mixed with regular chow for 4 or 14 days without injection of STZ (Gal4d and Gal14d groups). The eyes of these animals were monitored daily to assess the progression of cataract, using a previously published method [13]. At predefined times, the animals were killed and RNA was taken from their lenses.…”

Section: Animalsmentioning

confidence: 99%

“…In the lens, osmotic stress imposed by sorbitol accumulation has long been believed to be the major factor in the progression of diabetic cataract [6][7][8][9][10][11]. Activation of the AR/sorbitol pathway also increases oxidative stress by increasing levels of hydrogen peroxide [12] and free radicals [13]. The involvement of the polyol pathway in generation of hyperglycaemia-induced oxidative stress has been demonstrated in diabetic lenses [7,8,14].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Gene expression profiling of diabetic and galactosaemic cataractous rat lens by microarray analysis

2005

View full text Add to dashboard Cite

Aims/hypothesis: Osmotic and oxidative stress is associated with the progression and advancement of diabetic cataract. In the present study, we used a cDNA microarray method to analyse gene expression patterns in streptozotocininduced diabetic rats and galactose-fed cataractous lenses. In addition, we investigated the regulation and interaction(s) of anti-oxidant protein 2 and lens epithelium-derived growth factor in these models. Methods: To identify differential gene expression patterns, one group of Sprague-Dawley rats was made diabetic with streptozotocin and a second group was made galactosaemic. Total RNA was extracted from the lenses of both groups and their controls. Labelled cDNAwas hybridised to Atlas Rat Arrays. Changes in gene expression level were analysed. Real-time PCR and western analysis were used to validate the microarray results. Results: The expression of 31 genes was significantly modulated in hyperglycaemic lenses compared with galactosaemic lenses. Notably, transcript and protein levels of B-cell leukaemia/ lymphoma protein 2 and nuclear factor-κB were significantly elevated in rat lenses at 4 weeks after injection of streptozotocin. At a later stage, mRNA and protein levels of TGF-β were elevated. However, levels of mRNA for IGF-1, lens epithelium-derived growth factor and anti-oxidant protein 2 were diminished in streptozotocin-induced diabetic cataract. Conclusions/interpretations: These results provide evidence that progression of sugar cataract involves oxidativeand TGF-β-mediated signalling. These pathways may promote abnormal gene expression in the hyperglycaemic and galactosaemic states and thus may contribute to the symptoms associated with these conditions. Since oxidative stress seems to be a major event in cataract formation, supply of anti-oxidant may postpone the progression of such disorders.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Gene expression profiling of diabetic and galactosaemic cataractous rat lens by microarray analysis

2005

View full text Add to dashboard Cite

show abstract

“…Kubo et al . [ 32 ] have shown that hydroxyl radical formation occurs in accordance with accumulation of galactitol, a polyol, converted from galactose via the polyol pathway in the cataractous lens of galactose-fed rats, and have suggested that hydroxyl radical may play an important role in the early stage of galactose cataract process. Thus, it has been implicated that oxidative stress contributes to cataract development in various cataract models of experimental animals as well as in humans.…”

Section: Relationship Between Cataracts and Oxidative Stressmentioning

confidence: 99%

Possibility of Clinical Application of Vitamin E to Cataract Prevention

Ohta

2004

J. Clin. Biochem. Nutr.

View full text Add to dashboard Cite

Summary It has been implicated that oxidative stress is involved in the development of aged-related and diabetic cataracts in humans and also in cataract development in a variety of in vivo experimental cataract models. Therefore, this article will review the possibility of the clinical application of vitamin E to cataract prevention, based on data concerning the level of vitamin E in normal and cataractous lenses of humans and experimental animals, the relationship between dietary vitamin E intake and the risk of cataracts, the effect of vitamin E supplementation on cataract development in humans, and the effect of oral or parenteral vitamin E treatment or topical vitamin E instillation on cataract development in a variety of in vivo experimental cataract models. These data reported so far may allow us to think of a possibility that vitamin E is clinically applied to cataract development.

show abstract

“…Since we have established a precise method for evaluating rat cataract by using two types of digital camera systems that are equipped with a non-reflecting illuminator 2) or a slit lamp. Oxidative stress is now recognized as a major factor involved in the cataract formation induced by a variety of factors including impairment of systemic metabolism such as diabetes mellitus, [3][4][5][6][7][8][9] aging, 10,11) UV irradiation, 12) and photochemical insults. 13) Although the most frequent cause of cataracts is the usual aging process, 1) it often occurs earlier in life with diabetes mellitus.…”

mentioning

confidence: 99%

Attenuation of Cataract Progression by A-3922, a Dihydrobenzofuran Derivative, in Streptozotocin-Induced Diabetic Rats

Saito

Ueo

Kametaka

et al. 2008

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

The present study was undertaken to assess whether A-3922, a dihydrobenzofuran derivative that possesses antioxidative effects, had any preventive effect on the onset and/or progression of diabetic cataract. Male Wistar rats were received a bolus intravenous injection of streptozotocin (65 mg/kg) and were given 5% glucose in drinking water for 10 weeks. The diabetic rats were divided into two groups and treated with 30 mg/kg/d A-3922 or vehicle during the experimental period. The opacities of eye lenses were observed by using both our original device and a slit lamp microscope. The lens opacities were initially detected as early as the 2nd week and the cataracts were developed in similar fashion in both A-3922-treated and untreated diabetic rats until 7th week, suggesting that A-3922 did not show any appreciable effect on the onset of diabetic cataract. In the later period (8th week or later), however, progression of cataract was retarded and significant reductions in both the total cataract score and the degree of opacity were apparently observed on 10th week of A-3922-treated diabetic rats. These results suggest that A-3922 can delay the progression but not the onset of diabetic cataract, and it has a possibility to be a candidate for drugs of cataract associated with diabetes.

show abstract

Cataract Formation through the Polyol Pathway is associated with Free Radical Production

Cited by 67 publications

References 18 publications

Gene expression profiling of diabetic and galactosaemic cataractous rat lens by microarray analysis

Gene expression profiling of diabetic and galactosaemic cataractous rat lens by microarray analysis

Possibility of Clinical Application of Vitamin E to Cataract Prevention

Attenuation of Cataract Progression by A-3922, a Dihydrobenzofuran Derivative, in Streptozotocin-Induced Diabetic Rats

Contact Info

Product

Resources

About