Catalytic synthesis and antitumor activities of sulfated polysaccharide from Gynostemma pentaphyllum Makino

Chen, Tong; Li, Bo; Li, Yuanyuan; Zhao, Chuande; Shen, Jianmin; Zhang, Haixia

doi:10.1016/j.carbpol.2010.08.024

Cited by 51 publications

(19 citation statements)

References 25 publications

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“…It had been demonstrated that a moderate DSS was necessary for a high antitumor activity in vitro (Chen et al, 2011). We also found that when the DSS was within the range of 0.41-1.02, the derivatives exhibited relatively stronger antitumor activity in vitro.…”

Section: Antitumor Activities Of Csps-1 and Csps-1-smentioning

confidence: 63%

“…We also found that when the DSS was within the range of 0.41-1.02, the derivatives exhibited relatively stronger antitumor activity in vitro. This might be due to the fact that the sulfated polysaccharides with high DSS are often accompanied with low molecular weight and damaged structure of polysaccharides resulted from longer reaction time in the strong acidic environment (Chen et al, 2011;Yang, Du, Wen, Li, & Hu, 2003). Therefore, the DSS of polysaccharides must be within the optimal ranges.…”

Section: Antitumor Activities Of Csps-1 and Csps-1-smentioning

confidence: 98%

“…Sulfation of polysaccharides can enhance water solubility, resulting in alteration of their biological activities (Wang et al, 2010). It has been reported that sulfated polysaccharides exhibit potent biological properties in comparison with non-sulfated polysaccharides, such as anti-viral, anti-oxidant and anti-tumor activities (Chen et al, 2011;Karmakar, Pujol, Damonte, Ghosh, & Ray, 2010;Liu, Wan, Shi, & Lu, 2011;Zou, Du, Li, Yang, & Zhang, 2010). Therefore, sulfation can be considered as an effective way to enhance the biological activities of polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Structural characterization, sulfation and antitumor activity of a polysaccharide fraction from Cyclina sinensis

Jiang

Xiong

et al. 2015

Carbohydrate Polymers

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Section: Antitumor Activities Of Csps-1 and Csps-1-smentioning

confidence: 63%

Section: Antitumor Activities Of Csps-1 and Csps-1-smentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Structural characterization, sulfation and antitumor activity of a polysaccharide fraction from Cyclina sinensis

Jiang

Xiong

et al. 2015

Carbohydrate Polymers

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“…The similar study undertaken by Tong Chen indicated that the sulfated polysaccharide from Gynostemma pentaphyllum Makino (GPP) inhibited the growth of HepG2 cells in vitro significantly. Especially, at the concentration of 2,000 μg/ml, the inhibition activity of the sulfated polysaccharide (GPP2-s4) was the highest with a viability of 53.4% [31], but it is relatively lower than that of SF-CRFP.…”

Section: Anti-tumor Activity Of Crfp and Its Derivativesmentioning

confidence: 98%

In Vitro Anti-tumor Effects of Chemically Modified Polysaccharides from Cherokee Rose Fruit

Xiao

Zhang

Liu

et al. 2014

International Journal of Food Engineering

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Cherokee rose fruit (CRF) is a Chinese traditional herb which has been used in medicine for hundreds of years. The anti-tumor activity of CRF polysaccharides (CRFPs) has not yet been evaluated. To study the in vitro anti-tumor effects of CRFP and its derivatives, native CRFP was isolated from CRF by hot water extraction, and its molecular weight analyzed with gel filtration chromatography was 227,000 Da. Native CRFP was sulfated with ClSO3H-DMF and carboxymethylated with monochloroacetic acid in alkaline aqueous medium. The resulting derivatives were isolated and labeled as SF-CRFP and CM-CRFP, respectively. The in vitro inhibition rates of CRFP and its derivatives for tumor cells SKVO (human ovarian cancer cell), HepG2 (human hepatoma cell), and LoVo (human colon cancer cell) were evaluated, the result showed that native CRFP exhibited no significant inhibition effect on the three tumor cells even at a concentration of 50 μg/ml, but sulfation and carboxymethylation substantially enhanced the anti-tumor activities of CRFP in a dose-dependent way. SF-CRFP at the dose of 50 μg/ml displayed a significant inhibitory effect on SKVO, HepG2, and LoVo, with the viability rates of 33.6%, 44.8%, and 43.2%, respectively. It has a dosage-dependence inhibition on tumor growth in this model, with IC50 for SKVO, HepG 2, and LoVo being 21 μg/ml, 36 μg/ml, and 49 μg/ml, respectively. CM-CRFP showed a specific inhibition on HepG2 with a viability rate of 12.2%, with an IC50 of 17 μg/ml, while it had hardly any antitumor effect on SKVO cells. Thus, chemical modifications of CRFPs by sulfation and carboxymethylation effectively improved their anti-tumor properties.

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“…Wang and Luo (2007), report that crude PGP exhibited scavenging capacities against hydroxyl radical and superoxide radicals. Chen et al (2011), demonstrated that the sulfated polysaccharide from GP have distinct antitumor activities. Chi et al (2012), found that PGP could increase antioxidant enzyme activities some of antioxidant enzyme activities, and decrease MDA level in muscle of exercised mice.…”

Section: Introductionmentioning

confidence: 99%

Effects of Polysaccharides from <i>Gynostemma Pentaphyllum</i> (Thunb.), Makino on Physical Fatigue

Lin-Na¹,

Yong-Xiu²

2014

Afr. J. Trad. Compl. Alt. Med.

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Background: Gynostemma pentaphyllum (Thunb.) Makino has been reported to have a wide range of health benefits in Chinese herbal medicines. Polysaccharides from Gynostemma pentaphyllum (PGP), has been identified as one of the active ingredients responsible for its biological activities. Although many pharmacological activities of PGP have received a great deal of attention, there is limited evidence for the anti-fatigue effects of PGP. The purpose of this study was to investigate the effects of polysaccharides from PGP on physical fatigue. Materials and method: The rats were divided into four groups, with 10 animals per group: control (C), group, low-treated (LT), group, mediumtreated (MT), group, and high-treated (HT), group. The C group received distilled water, while LT, MT and HT groups were given various doses of PGP (100, 200, 400 mg/kg· d). After 30 days, forced swimming test was carried out in an acrylic plastic pool, then the exhaustive swimming time of rats and some biochemical parameters related to fatigue were measured. The data obtained showed that PGP could extend the exhaustive swimming time of the rats, as well as decrease the blood lactic acid (BLA), and blood urea nitrogen (BUN), concentrations, and increase the hemoglobin, liver glycogen and muscle glycogen concentrations. Result: The data obtained showed that different doses of PGP could extend the exhaustive swimming time of the rats, as well as decrease the BLA and BUN concentrations, and increase the hemoglobin, liver glycogen and muscle glycogen concentrations, which suggests that PGP had significant anti-fatigue effects on rats. Conclusion: PGP may be of use as a potential anti-fatigue agent, but there is a need for further research on long-term use in order to show its positive effects on physical fatigue.

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Catalytic synthesis and antitumor activities of sulfated polysaccharide from Gynostemma pentaphyllum Makino

Cited by 51 publications

References 25 publications

Structural characterization, sulfation and antitumor activity of a polysaccharide fraction from Cyclina sinensis

Structural characterization, sulfation and antitumor activity of a polysaccharide fraction from Cyclina sinensis

In Vitro Anti-tumor Effects of Chemically Modified Polysaccharides from Cherokee Rose Fruit

Effects of Polysaccharides from <i>Gynostemma Pentaphyllum</i> (Thunb.), Makino on Physical Fatigue

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