Catalytic route electrochemiluminescence microscopy of cell membranes with nitrogen-doped carbon dots as nano-coreactants

“…Since the diffusion length of Ru(bpy) 3 3+ extends over the entire cell, intracellular structures in the whole cell can be imaged. Alternatively, labeling the cell membrane with coreactant also enables ECL imaging of the entire cell membrane via the catalytic route [62] …”

Section: Ecl Generation In Solutionsmentioning

confidence: 99%

Deciphering the Mechanisms of Electrochemiluminescence by Spatially Resolved Measurements

Wang

2021

Analysis & Sensing

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Elucidating the mechanisms of electrochemiluminescence (ECL) generation is crucial for developing novel ECL systems, designing new luminophores and coreactants, and expanding the application of ECL in different fields. Diverse methods have been developed so far for this purpose, among which spatially resolved measurements using ECL microscopy (ECLM), scanning electrochemical microscopy (SECM) and ECL self‐interference spectroscopy (ECLIS), are particularly valuable in terms of their ability to measure the distribution of excited luminophores and coreactant radicals. This review aims to provide an overview of research progress on deciphering the mechanisms of ECL by spatially resolved measurements. The reaction pathways for ECL generation are briefly introduced and then the advances in understanding the mechanisms of ECL reaction in solutions and on the surface of microbeads are summarized. A personal perspective covering both mechanisms and challenges is finally presented.

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“…Ma et al. employed NCDs modified with PSBP peptide to construct novel nanostructured interfaces where PSBP could recognize externalized phosphatidylserine (PS) on the membranes of apoptotic cells and NCDs reacted with Ru(bpy) 3 2+ to emit light [52]. The ECL images reflected the distribution of PS on the cell surface at different heights.…”

Section: Single‐cell Analysismentioning

confidence: 99%

“…NCDs with abundant amine moieties made the ECL reaction through a "catalytic route" which lead to an extended ECL layer resulting in the imaging of the upper cell membrane. Ma et al employed NCDs modified with PSBP peptide to construct novel nanostructured interfaces where PSBP could recognize externalized phosphatidylserine (PS) on the membranes of apoptotic cells and NCDs reacted with Ru(bpy) 3 2 + to emit light [52]. The ECL images reflected the distribution of PS on the cell surface at different heights.…”

Section: Membrane Protein Analysismentioning

confidence: 99%

Electrochemiluminescence Single‐cell Analysis on Nanostructured Interface

2021

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Due to the heterogeneity of single cells, the development of single-cell analysis methods is conducive to the research of cellular mechanisms, clinical diagnosis, and treatment. Electrochemiluminescence (ECL) has the advantage of being controllable in time and space. Compared with spectroscopy, ECL does not require a light source, thus avoiding the interference of scattered light and luminescent impurities. Therefore, ECL is playing an increasingly important role in the field of single-cell analysis. In ECL single-cell analysis, it is important to construct a suitable nanostructure interface to realize signal conversion. This review first briefly introduced the ECL system commonly used in single-cell analysis, then focused on the recent developments in ECL single-cell analysis on nanostructure interface, finally discussed the future challenges and outlooks of ECL single-cell analysis.

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Catalytic route electrochemiluminescence microscopy of cell membranes with nitrogen-doped carbon dots as nano-coreactants

Abstract: Catalytic route electrochemiluminescence microscopy enables us to image upper cell membranes with a vertical resolution mode by using nitrogen-doped carbon dots as nano-coreactants and labels.

Cited by 38 publications

References 32 publications

Recent progress in electrochemiluminescence microscopy analysis of single cells

Recent progress in electrochemiluminescence microscopy analysis of single cells

Deciphering the Mechanisms of Electrochemiluminescence by Spatially Resolved Measurements

Electrochemiluminescence Single‐cell Analysis on Nanostructured Interface

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