Catalytic properties and polymorphism of serine endopeptidases from the midgut gland of the brown shrimp Crangon crangon (Decapoda, Caridea)

Saborowski, Reinhard; Schatte, Jessica; Giménez, Luis

doi:10.1007/s00227-012-1890-0

Cited by 15 publications

(8 citation statements)

References 27 publications

(48 reference statements)

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“…In salmon (Salmon salar), different trypsin isoenzymes can have an effect on physiological conditions such as fish size, growth rate, protein utilization and feed efficiency [55]. Enzyme polymorphism has also been shown to be important for adaptation to a broad food spectrum in caridean shrimp (Crangon crangon) [56]. The results presented here demonstrate that cod trypsin ZT and cod trypsin I show differences in stability to pH and heat, sub-site substrate specificity and sensitivity to inhibitors.…”

Section: Discussionmentioning

confidence: 99%

Biochemical characterization of a native group III trypsin ZT from Atlantic cod (Gadus morhua)

Sandholt

Stefansson²,

Scheving³

et al. 2019

International Journal of Biological Macromolecules

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Atlantic cod trypsin ZT is biochemically characterized for the first time in this report in comparison to a group I trypsin (cod trypsin I). To our knowledge, trypsin ZT is the first thoroughly characterized group III trypsin. A more detailed understanding of trypsin ZT biochemistry may give insight into its physiological role as well as its potential use within the biotechnology sector. Stability is an important factor when it comes to practical applications of enzymes. Compared to trypsin I, trypsin ZT shows differences in pH and heat stability, sensitivity to inhibitors and sub-site substrate specificity as shown by multiplex substrate profiling analysis. Based on the analysis, trypsin ZT cleaved at arginine and lysine as other trypsins. Furthermore, trypsin ZT is better than trypsin I in cleaving peptides containing several consecutive positively charged residues. Lysine-and arginine-rich amino acid sequences are frequently found in human viral proteins. Thus, trypsin ZT may be effective in inactivating human and fish viruses implying a possible role for the enzyme in the natural defence of Atlantic cod. The results from this study can lead to multiple practical applications of trypsin ZT.

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Section: Discussionmentioning

confidence: 99%

Biochemical characterization of a native group III trypsin ZT from Atlantic cod (Gadus morhua)

Sandholt

Stefansson²,

Scheving³

et al. 2019

International Journal of Biological Macromolecules

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“…Peroxidase (POD: EC 1.15.1.1) activity was measured at 470 nm by calculating the oxidation of O-dianisidine in the presence of H 2 O 2 , and the enzyme activity was articulated as ∆OD 470 /min/g FW [59]. Polyphenol oxidase (PPO: EC 1.10.3.1) activity was measured at 420 nm and expressed as the unit ∆OD 420 /min/g FW, based on the oxidation of nicotinamide adenine dinucleotide phosphate (NADPH) [60].…”

Section: Enzyme Activity Assaysmentioning

confidence: 99%

New Lead Discovery of Herbicide Safener for Metolachlor Based on a Scaffold-Hopping Strategy

et al. 2020

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The use of herbicide safeners can significantly alleviate herbicide injury to protect crop plants and expand the application scope of the existing herbicides in the field. Sanshools, which are well known as spices, are N-alkyl substituted compounds extracted from the Zanthoxylum species and have several essential physiological and pharmacological functions. Sanshools display excellent safener activity for the herbicide metolachlor in rice seedlings. However, the high cost of sanshools extraction and difficulties in the synthesis of their complicated chemical structures limit their utilization in agricultural fields. Thus, the present study designed and synthesized various N-alkyl amide derivatives via the scaffold-hopping strategy to solve the challenge of complicated structures and find novel potential safeners for the herbicide metolachlor. In total, 33 N-alkyl amide derivatives (2a–k, 3a–k, and 4a–k) were synthesized using amines and saturated and unsaturated fatty acids as starting materials through acylation and condensation. The identity of all the target compounds was well confirmed by 1H-NMR, 13C-NMR, and high-resolution mass spectrometry (HRMS). The primary evaluation of safener activities for the compounds by the agar method indicated that most of the target compounds could protect rice seedlings from injury caused by metolachlor. Notably, compounds 2k and 4k displayed excellent herbicide safener activities on plant height and demonstrated relatively similar activities to the commercialized compound dichlormid. Moreover, we showed that compounds 2k and 4k had higher glutathione S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and polyphenol oxidase (PPO) activities in rice seedlings, compared to the metolachlor treatment. In particular, 2k and 4k are safer for aquatic organisms than dichlormid. Results from the current work exhibit that compounds 2k and 4k have excellent crop safener activities toward rice and can, thus, be promising candidates for further structural optimization in rice protection.

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“…C. crangon may occur in high numbers of up to 82 individuals per m 2 , including juveniles (Boddeke et al, 1986), but population densities show pronounced interannual variations (Hünerlage et al, 2019). Ecophysiological adaptations allow C. crangon to cope with the variable environmental conditions of the North Sea, including strong fluctuations in temperature, salinity, and food availability (Campos & van der Veer, 2008;Saborowski et al, 2012;Reiser et al 2014a,b;Martínez-Alarcón et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Sand grains in the stomach of brown shrimp, Crangon crangon: Crunchy garnish, supportive macerator, or simply dirt?

Schmidt

Korez

Saborowski

et al. 2021

Journal of Sea Research

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Brown shrimp, Crangon crangon, inhabit highly productive sandy and muddy grounds of the southern North Sea. The stomachs of the shrimp contain variable and often high numbers of sediment grains. The function of sediment grains inside the stomach and the purpose of their ingestion are only poorly understood. We tested in laboratory experiments whether sediment and associated organic material complement the natural food of C. crangon or if sand grains may be used by the shrimp to support trituration and maceration of ingested food. The shrimp showed no notable preference for sediment with natural organic content over sediment with reduced organic content, limited ingestion of sediment upon starvation, and no additional uptake of sand grains after feeding. Instead, C. crangon took up sediment only while feeding on regular food, suggesting that sand grains are not ingested intentionally but rather incidentally as a side effect of hasty gobbling. This conclusion is supported by the highly variable uptake of sand grains among individuals. Under experimental conditions, sand grains from sediments do not seem to have a crucial function in food processing and digestion in brown shrimp.

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Catalytic properties and polymorphism of serine endopeptidases from the midgut gland of the brown shrimp Crangon crangon (Decapoda, Caridea)

Cited by 15 publications

References 27 publications

Biochemical characterization of a native group III trypsin ZT from Atlantic cod (Gadus morhua)

Biochemical characterization of a native group III trypsin ZT from Atlantic cod (Gadus morhua)

New Lead Discovery of Herbicide Safener for Metolachlor Based on a Scaffold-Hopping Strategy

Sand grains in the stomach of brown shrimp, Crangon crangon: Crunchy garnish, supportive macerator, or simply dirt?

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