Catalytic Hairpin Assembly-Programmed Porphyrin–DNA Complex as Photoelectrochemical Initiator for DNA Biosensing

Abstract

A catalytic hairpin assembly (CHA)-programmed porphyrin-DNA complex was designed to trigger the chemiluminescence as photoelectrochemical initiator for DNA sensing. First, the programmed double strand DNA (dsDNA) was formed using two hairpin DNAs as assembly components via target-assisted CHA reaction, and then immobilized on a capture DNA/CdS quantum dots modified electrode. The porphyrin (FeTMPyP) was conveniently assembled on a dsDNA scaffold via the groove interaction. The FeTMPyP@dsDNA complex possessed high catalytic activity toward luminol oxidation to generate the desirable chemiluminescence with high stability under various temperature and alkaline conditions. By integrating the signal amplification capacity of CHA and in situ FeTMPyP-mediated chemiluminescence as excitation light, an amplified photoelectrochemical sensing strategy is proposed for DNA detection. Under optimized conditions, the biosensor shows a wide linear range from 5 to 10000 fM with a detection limit of 2.2 fM. Moreover, the developed photoelectrochemical device exhibits excellent selectivity, high stability, and acceptable fabrication reproducibility. The CHA-programmed porphyrin-DNA strategy not only extends the applications of photoelectrochemistry, but also presents a novel methodology in bioanalysis.

A visible light induced photoelectrochemical aptsensor constructed by aligned ZnO@CdTe core shell nanocable arrays/carboxylated g-C3N4 for the detection of Proprotein convertase subtilisin/kexin type 6 gene

“…It is one of the most widely and early used method. It is applied in the biological and environmental analysis, such as protein (Zang et al 2015), cell (Primiceri et al 2010;, biological molecule (Beitollahi et al 2008;Mazloum-Ardakani et al 2011;Mazloum-Ardakani et al 2012), metal ion (Pelossof et al 2012;Sener et al 2014). PEC assay is a new analytical method for the faster, cheaper and simpler nucleic acid assay.…”

A photoelectrochemical biosensor for determination of DNA based on flower rod-like zinc oxide heterostructures

“…The marriage of optical methods and electrochemical approaches endows PEC sensors with small detection backgrounds and greatly high sensitivity [3][4][5][6]. Owing to low background signal and desirable sensitivity, PEC bioassays have offered various elegant routes for biological recognition events [7,8]. In this respect, it provides a great choice for bioassay in an environmentally-friendly format.…”

β-Cyclodextrin as a Metal-anionic Porphyrin Complexation Accelerator in Aqueous Media

“…[1][2][3][4][5][6][7][8][9] In addition, metal-porphyrin complexes have various functions that are useful in analytical chemistry, and are also of interest to many other researchers, which has led to the use of metal-porphyrin complexes in applications such as optics, 10 chemical stacking, 11 catalysis, 12 electron transport, 13 oxidation-reduction reactions, 14 and biological mimicking substances. 15,16 Thus, numerous reports regarding the chemical function and applications of porphyrin derivatives have been published.…”