Catalytic ferromagnetic gold nanoparticle immunoassay for the detection and differentiation of Mycobacterium tuberculosis and Mycobacterium bovis

Gilbride, Brendan; Moreira, Gustavo Marçal Schmidt Garcia; Hust, Michael; Cao, Cuong; Stewart, Linda

doi:10.1016/j.aca.2021.339037

Cited by 7 publications

(6 citation statements)

References 47 publications

(54 reference statements)

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“…It has been proved that the protein concentration had a certain effect on the enzyme activity of nanomaterials. 29,30 In this work, the suppression of enzyme activity became increasingly apparent as the protein concentration increased (Figure S2A,B). As shown in Figure S2A, the catalytic activity of the VS 2 NS before and after coating of antibodies does not show significant difference (bars 1 and 2) in the absence of BSA.…”

Section: Enhanced Lfia Resultsmentioning

confidence: 57%

Vanadium Disulfide Nanosheet Boosts Optical Signal Brightness as a Superior Enzyme Label to Improve the Sensitivity of Lateral Flow Immunoassay

et al. 2022

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The color-enzyme lateral flow immunoassay (LFIA) has attracted widespread attention to expand the detection range and improve sensitivity via amplifying the color signal after catalyzing the substrate. As a kind of layered transition-metal dichalcogenide (TMD), the vanadium disulfide nanosheet (VS2NS) possesses superior peroxidase-like catalytic activity. Here, a VS2NS was applied as an enzyme label in the LFIA to detect 17β-estradiol (E2). Compared to natural horseradish peroxidase, the VS2NS expresses a more prominent enzyme catalytic performance, stability, and adsorption ability. Under optimal conditions, the calculated limit of detection (cLOD) of the VS2NS-based LFIA is 0.065 ng mL–1 for E2, which is sixfold lower than that of the optimized colloidal nanoparticle-based LFIA (cLOD = 0.406 ng mL–1). Besides, the detection linear range of the VS2NS-based LFIA can be widened by 1.5 times after the catalytic reaction. Moreover, the VS2NS-based LFIA exhibits excellent practicability in real sample detection. Simultaneously, this study helps open up the application of the VS2NS in the trace analysis of LFIAs, which can broaden TMDs’ scope of application and better show their properties of color enzymes.

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Section: Enhanced Lfia Resultsmentioning

confidence: 57%

Vanadium Disulfide Nanosheet Boosts Optical Signal Brightness as a Superior Enzyme Label to Improve the Sensitivity of Lateral Flow Immunoassay

et al. 2022

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“…In addition, both mycobacteria stain as acid-fast bacilli, are 99.95% genome-wide similar and have identical 16S rRNA sequences. Differentiating between them is crucial because M. bovis is intrinsically resistant to pyrazinamide, one of the commonly used first-line anti-TB drugs [ 121 ].…”

Section: Diagnostic Immunological Testsmentioning

confidence: 99%

Evolution of Laboratory Diagnosis of Tuberculosis

Zaporojan,

Negrean,

Hodișan

et al. 2024

Clinics and Practice

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Tuberculosis (TB) is an infectious disease of global public health importance caused by the Mycobacterium tuberculosis complex. Despite advances in diagnosis and treatment, this disease has worsened with the emergence of multidrug-resistant strains of tuberculosis. We aim to present and review the history, progress, and future directions in the diagnosis of tuberculosis by evaluating the current methods of laboratory diagnosis of tuberculosis, with a special emphasis on microscopic examination and cultivation on solid and liquid media, as well as an approach to molecular assays. The microscopic method, although widely used, has its limitations, and the use and evaluation of other techniques are essential for a complete and accurate diagnosis. Bacterial cultures, both in solid and liquid media, are essential methods in the diagnosis of TB. Culture on a solid medium provides specificity and accuracy, while culture on a liquid medium brings rapidity and increased sensitivity. Molecular tests such as LPA and Xpert MTB/RIF have been found to offer significant benefits in the rapid and accurate diagnosis of TB, including drug-resistant forms. These tests allow the identification of resistance mutations and provide essential information for choosing the right treatment. We conclude that combined diagnostic methods, using several techniques and approaches, provide the best result in the laboratory diagnosis of TB. Improving the quality and accessibility of tests, as well as the implementation of advanced technologies, is essential to help improve the sensitivity, efficiency, and accuracy of TB diagnosis.

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“…Another important application of GNP is in TB diagnosis. A ferromagnetic GNP-based immune detection system was developed for the detection of M. tuberculosis and to differentiate M. bovis [220]. GNPs loaded with quadruplex DNA motifs can aid in the diagnosis of M. tuberculosis in sputum [15,221].…”

Section: Gold Nanoparticlesmentioning

confidence: 99%

Advanced drug delivery and therapeutic strategies for tuberculosis treatment

Nair,

Greeny,

Nandan

et al. 2023

J Nanobiotechnol

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Tuberculosis (TB) remains a significant global health challenge, necessitating innovative approaches for effective treatment. Conventional TB therapy encounters several limitations, including extended treatment duration, drug resistance, patient noncompliance, poor bioavailability, and suboptimal targeting. Advanced drug delivery strategies have emerged as a promising approach to address these challenges. They have the potential to enhance therapeutic outcomes and improve TB patient compliance by providing benefits such as multiple drug encapsulation, sustained release, targeted delivery, reduced dosing frequency, and minimal side effects. This review examines the current landscape of drug delivery strategies for effective TB management, specifically highlighting lipid nanoparticles, polymer nanoparticles, inorganic nanoparticles, emulsion-based systems, carbon nanotubes, graphene, and hydrogels as promising approaches. Furthermore, emerging therapeutic strategies like targeted therapy, long-acting therapeutics, extrapulmonary therapy, phototherapy, and immunotherapy are emphasized. The review also discusses the future trajectory and challenges of developing drug delivery systems for TB. In conclusion, nanomedicine has made substantial progress in addressing the challenges posed by conventional TB drugs. Moreover, by harnessing the unique targeting abilities, extended duration of action, and specificity of advanced therapeutics, innovative solutions are offered that have the potential to revolutionize TB therapy, thereby enhancing treatment outcomes and patient compliance. Graphical Abstract

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Catalytic ferromagnetic gold nanoparticle immunoassay for the detection and differentiation of Mycobacterium tuberculosis and Mycobacterium bovis

Cited by 7 publications

References 47 publications

Vanadium Disulfide Nanosheet Boosts Optical Signal Brightness as a Superior Enzyme Label to Improve the Sensitivity of Lateral Flow Immunoassay

Vanadium Disulfide Nanosheet Boosts Optical Signal Brightness as a Superior Enzyme Label to Improve the Sensitivity of Lateral Flow Immunoassay

Evolution of Laboratory Diagnosis of Tuberculosis

Advanced drug delivery and therapeutic strategies for tuberculosis treatment

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