Catalytic Enzyme Activity Concentration in Plasma of Man, Sheep, Dog, Cat, Rabbit, Guinea Pig, Rat and Mouse. Approach to a Quantitative Diagnostic Enzymology. I. Communication

Abstract

Summary:For the most convenient laboratory animals in experimental medicine such äs the sheep, dog, cat, rabbit, guinea pig, rat and mouse, the catalytic concentrations of seventeen enzymes and the protein and albumin concentrations in plasma were determined. The corresponding data for man were taken from the literature. The experimental conditions were selected so äs to minimize the influence on catalytic activity, variance and statistical distribution pattern of such factors äs handling procedures, the choice of blood drawing technique, the choice of anaesthetic agent for distinct species and the preparation procedure of plasma from the blood specimen. Because these considerations have been largely neglected to date in experimental enzymology, normal values for most enzymes require revision and were in some instances established for the first time in certain species. Our use of assay conditions, which were identical for all animal species, satisfies current requirements in clinical chemistry with respect to standardization and optimization of enzyme catalytic activity concentration measurement, and allowed comparison of these enzymes. The catalytic activities established this way will be the basis for a later comparative study of their kinetics of adjustment in plasma. Katalytische Konzentration von Enzymen im Plasma von Mensch und Versuchstieren Versuch der Begründung einer quantitativen Diagnostisch-!· Enzymologie, I. Zusammenfassung: Bei den in der experimentellen

Effect of Feeding Olive by-Products on Certain Blood Parameters and Serum Enzyme Activities of Fattening Rabbits

“…It would be of considerable practical value to continue feeding the rabbits with the same levels of dehydrated olive cake pulp in fodder mixes (for several months or even years) and to monitor the influence of that feed component on serum AP activity. The values of AP activity found in this study fully correspond to those quoted as physiological for rabbits by Grötsch and Hajdu (1979), Lindena and Trautschold (1986), and Hewit et al (1989). However, the values found by us are higher than those reported by Dabew et al (1976) and Yu et al (1979), and lower than those published by Liker et al (1998).…”

“…It can therefore be concluded that consumption of feed containing different levels of dehydrated olive cake pulp caused no significant changes in serum GGT activity of rabbits after the 56-day period of experimental fattening. The GGT activities found in all groups of rabbits correspond to the values reported by Liker et al (1998), and are higher than those described by Yu et al (1979), Lindena and Trautschold (1986), and Lepitzki and Woolf (1991).…”

“…The serum LDH activity found in all groups of rabbits included in this experiment was higher than the values reported by Friedel and Mattenheimer (1970), Schievelbein et al (1970), Dabew et al (1976), Yu et al (1979), Lindena and Trautschold (1986) and Hewit et al (1989), they were equal to values published by Grötsch and Hajdu (1971), and lower than those reported by Liker et al (1998). That high level of serum LDH activity found in all groups of rabbits in this experiment was probably the result of a mild haemolysis that occurred during the taking of blood samples.…”

Activity of Some Enzymes in the Blood Plasma of Hylla Rabbits Fed Various Proportions of Substrate from the Production of the Pleurotus pulmonarius Mushroom

“…The CK activity in rabbits is considerably higher than in humans. Increased levels of CK, but also of LD and AST could be a consequence of the blood taking procedure, due to damaged muscle (Mitruka and Rawnstey 1981;Lindena and Trautschold 1986).…”

Biology and Diseases of Rabbits

“…As with any experiment, values can vary significantly between breeds, laboratories, methods of sampling and measurement, and individual rabbits due to age, sex, breed, health, handling, and husbandry (Hewitt et al, 1989;Lidena and Trautschold, 1986;Mitruka and Rawnsley, 1981;Wolford et al, 1986;Yu et al, 1979). It is important to recognize, however, that most of these values have been obtained through the study of adult New Zealand White rabbits.…”