Catalytic electrooxidation of NADH for dehydrogenase amperometric biosensors

Katakis, Ioanis; Domı́nguez, Elena

doi:10.1007/bf01242656

Cited by 195 publications

(131 citation statements)

References 133 publications

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“…The values of k obs were strongly dependent on [NADH]. This has been observed for virtually all mediator-modified electrodes used for catalytic NADH oxidation [4,5,44] and has been explained by the formation of a charge transfer (CT) complex before yielding the reaction products (see Reaction 3). Plots of 1/k obs vs.…”

Section: Electrocatalytic Oxidation Of Nadh At Dddpmodified Electrodesmentioning

confidence: 81%

NADH Oxidation Using Carbonaceous Electrodes Modified with Dibenzo‐Dithia‐Diazapentacene

et al. 2003

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The electrochemical and the electrocatalytic behavior for NADH oxidation of a newly synthesized phenothiazine derivative, 16H,18H-dibenzo[c,1]-7,9-dithia-16,18-diazapentacene (DDDP), adsorbed on solid spectrographic graphite and on graphite powder or on zirconium phosphate (ZP), both entrapped in carbon paste, were compared. Experiments using cyclic voltammetry and a rotating disk electrode, performed in different experimental conditions (various pH values and NADH concentrations), showed that i) in the pH range between 4 and 8, the E8× value for DDDP adsorbed on ZP becomes pH independent; ii) in contrast to other redox mediators, adsorption of DDDP on ZP induced a significant negative shift of the E8× value; iii) the reaction rate for electrocatalytic oxidation of NADH is markedly increased by adsorbing the mediator on ZP, being further improved by the presence of Ca 2 ions in the solution.

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Section: Electrocatalytic Oxidation Of Nadh At Dddpmodified Electrodesmentioning

confidence: 81%

NADH Oxidation Using Carbonaceous Electrodes Modified with Dibenzo‐Dithia‐Diazapentacene

et al. 2003

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“…As already mentioned, this may be important in many respects. It is also to note that the stability data are determined under different conditions and, in most cases, not well documented in the works described (Katakis and Domínguez 1997).…”

Section: Discussionmentioning

confidence: 98%

“…The retention can be achieved by mediator immobilization at the conducting surface. This review is focused on the applicability of immobilized mediators for the oxidation of reduced NAD(P)H. For more information about the electrochemical basics and mechanistic investigations, please read the following reviews: Gorton and Domínguez (2002), Katakis and Domínguez (1997), Kumar and Chen (2008), and Radoi and Compagnone (2009).…”

Section: Introductionmentioning

confidence: 99%

Immobilized redox mediators for electrochemical NAD(P)+ regeneration

Kochius

Magnusson

Hollmann

et al. 2012

Appl Microbiol Biotechnol

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The applicability of dissolved redox mediators for NAD(P) + regeneration has been demonstrated several times. Nevertheless, the use of mediators in solutions for sensor applications is not a very convenient strategy since the analysis is not reagentless and long stabilization times occur. The most important drawbacks of dissolved mediators in biocatalytic applications are interferences during product purification, limited reusability of the mediators, and their cost-intensive elimination from wastewater. Therefore, the use of immobilized mediators has both economic and ecological advantages. This work critically reviews the current stateof-art of immobilized redox mediators for electrochemical NAD(P) + regeneration. Various surface modification techniques, such as adsorption polymerization and covalent linkage, as well as the corresponding NAD(P) + regeneration rates and the operational stability of the immobilized mediator films, will be discussed. By comparison with other existing regeneration systems, the technical potential and future perspectives of biocatalytic redox reactions based on electrochemically fed immobilized mediators will be assessed.

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“…PQQ itself is also a good catalyst for oxidation and reduction of another common cofactor, nicotinamide adenine dinucleotide (NAD + ), which exhibits very irreversible electrochemistry [7,8].…”

Section: Introductionmentioning

confidence: 99%

Simple immobilization of pyrroloquinoline quinone on few-walled carbon nanotubes

Kanninen

Ruiz

Kallio

et al. 2010

Electrochemistry Communications

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Pyrroloquinoline quinone (PQQ) was immobilized on glassy-carbon electrodes (GCE) modified with single-walled carbon nanotubes (SWCNT), few-walled carbon nanotubes (FWCNT) and carbon black (Vulcan XC72R). Modified electrodes were prepared by drop-casting. Immobilization was achieved with an extremely simple dipping procedure and without any further modification to the electrodes. Electrochemical performance of the electrodes was studied by cyclic voltammetry and spectroelectrochemistry. FWCNT adsorbed 30 times more PQQ than the other carbon materials. Compared to more complicated immobilization methods, PQQ/FWCNT/GCE showed well-defined electrochemistry in a considerably wide pH area from 2 to 12. The dipping process is affected by pH and electrostatic forces. At dipping pH 9.5, where both FWCNTs and -2 -PQQ have strong negative charge, the adsorption was halved compared to dipping pH 2, where the charges are smaller.

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Catalytic electrooxidation of NADH for dehydrogenase amperometric biosensors

Cited by 195 publications

References 133 publications

NADH Oxidation Using Carbonaceous Electrodes Modified with Dibenzo‐Dithia‐Diazapentacene

NADH Oxidation Using Carbonaceous Electrodes Modified with Dibenzo‐Dithia‐Diazapentacene

Immobilized redox mediators for electrochemical NAD(P)+ regeneration

Simple immobilization of pyrroloquinoline quinone on few-walled carbon nanotubes

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