2017
DOI: 10.1021/acschembio.6b01140
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Catalytic Dyad in the SGNH Hydrolase Superfamily: In-depth Insight into Structural Parameters Tuning the Catalytic Process of Extracellular Lipase from Streptomyces rimosus

Abstract: SrLip is an extracellular enzyme from Streptomyces rimosus (Q93MW7) exhibiting lipase, phospholipase, esterase, thioesterase, and tweenase activities. The structure of SrLip is one of a very few lipases, among the 3D-structures of the SGNH superfamily of hydrolases, structurally characterized by synchrotron diffraction data at 1.75 Å resolution (PDB: 5MAL ). Its crystal structure was determined by molecular replacement using a homology model based on the crystal structure of phospholipase A from Streptomyces a… Show more

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Cited by 28 publications
(19 citation statements)
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“…6) includes seven ORFs, all cellulosomal. Four of them include GH9 and CBM3c’s, one enzyme with a GH43, a single dockerin-containing ORF and a putative SGNH_hydrolase (putative esterase or lipase [65]). This group of genes (Bccel_0518-22; Bccel_0526-27) encodes cellulosomal cellulases with similar architecture and most probably similar complementary functions.…”
Section: Resultsmentioning
confidence: 99%
“…6) includes seven ORFs, all cellulosomal. Four of them include GH9 and CBM3c’s, one enzyme with a GH43, a single dockerin-containing ORF and a putative SGNH_hydrolase (putative esterase or lipase [65]). This group of genes (Bccel_0518-22; Bccel_0526-27) encodes cellulosomal cellulases with similar architecture and most probably similar complementary functions.…”
Section: Resultsmentioning
confidence: 99%
“…Moreover, catalytic dyads lacking Asp in the DXXH motif are also identified in lipase from Streptomyces rimosus (PDB code: 5MAL) [39] and esterase from Streptomyces scabies (PDB code: 1ESC) as shown in Figure 4 [20]. In their structures, Asp residues in the catalytic triad are replaced by nonfunctional Asn in the S. rimosus lipase and Trp in the S. scabies esterase, in which both Asn and Trp only stabilize the orientation of the catalytic His instead of playing a role as acids.…”
Section: • Group 1-2mentioning
confidence: 99%
“…2QUA [86], 2QUB [86], 2QXT [87], 2QXU [87], 2W22 [88], 2Z5G [80], 2Z8X [89], 2Z8Z [89], 2ZJ6 [90], 2ZJ7 [90], 2ZVD [91], 3A6Z [91], 3A70 [91], 3AUK, 3D2A [92], 3D2B [92], 3D2C [92], 3LIP [83], 3QMM [93], 3QZU [94], 3UMJ [95], 3W9U, 4FDM [96], 4FKB, 4FMP, 4GW3 [97], 4GXN [97], 4HS9 [97], 4LIP [98], 4OPM, 4X6U [99], 4X71 [99], 4X7B [99], 4X85 [99], 5AH1 [100], 5CE5 [100], 5CRI [101], 5CT4 [101], 5CT6 [101], 5CT9 [101], 5CTA [101], 5CUR [101], 5H6B [102], 5LIP [98], 5MAL 2 [39], 5XPX, 6A12 [103], 6CL4 [103], 6FZ1 [104], 6FZ7 [104], 6FZ8 [104], 6FZ9 [104], 6FZA [104], 6FZC …”
Section: Introductionmentioning
confidence: 99%
“…However, the Asp/Glu at this position is not conserved and thus it is not included in the name. Residues other than Asp/Glu often make the catalytic triad degrade to a catalytic dyad (8). It is worth noting that the residue of VvPlpA at this position is a Gly.…”
mentioning
confidence: 99%