Catalytic durability of magnetoproteoliposomes captured by high-gradient magnetic forces in a miniature fixed-bed reactor

Cuyper, Marcel De; Meulenaer, Bruno De; Meeren, Paul Van der; Vanderdeelen, Jean

doi:10.1002/(sici)1097-0290(19960320)49:6<654::aid-bit6>3.0.co;2-n

Cited by 15 publications

(6 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…70%, similar to that of the enzyme in unpolymerized BMPC vesicles. The enhanced stabilities of membrane enzymes embedded in phospholipid bilayer membranes have been observed by many workers [31,32]. The present results show that the stability is further improved by polymerization of the lipid membranes.…”

Section: Resultssupporting

confidence: 84%

Novel support for membrane enzyme immobilization: gel beads containing polymerized phospholipid vesicles

Gotoh

Iwanaga

Kikuchi

et al. 1998

Biotech and App Biochem

View full text Add to dashboard Cite

The present study has demonstrated a novel immobilization support for membrane enzymes; the support is composed of agarose gel beads and polymerized phospholipid vesicles contained within the beads. A phosphatidylcholine analogue, bis‐[12‐(methacryloyloxy)dodecanoyl]‐L‐α‐phosphatidylcholine (BMPC) [Regen, Singh, Oehme and Singh (1982) J. Am. Chem. Soc. 104, 791–795], was contained in Sepharose CL‐6B beads through the formation and simultaneous entrapment of vesicles by a combination of phospholipid solubilization in organic solvent with the beads, complete removal of the solvent and sonication in a buffer. The vesicle membranes were then polymerized by UV irradiation, which stabilized the hybrid‐type support. γ‐Glutamyl transpeptidase, a membrane enzyme from bovine kidney, was immobilized in the beads by reconstitution in the polymerized BMPC vesicles contained within the beads. The enzyme catalytic activity, as indicated by apparent Michaelis‐Menten kinetics, was almost identical with that of the enzyme reconstituted in unpolymerized BMPC vesicles. Polymerized BMPC vesicles significantly stabilized the enzyme to heat treatment when compared with unpolymerized BMPC vesicles and egg yolk phospholipid liposomes.

show abstract

Section: Resultssupporting

confidence: 84%

Novel support for membrane enzyme immobilization: gel beads containing polymerized phospholipid vesicles

Gotoh

Iwanaga

Kikuchi

et al. 1998

Biotech and App Biochem

View full text Add to dashboard Cite

show abstract

“…Compared to their iron oxide-free counterparts, MLs demonstrate some added values. First, since the inner space is completely filled up with the iron oxide onto which the lipid bilayer is strongly chemisorbed, MLs are impervious to osmotic pressure [11]. Second, the presence of the iron oxide core can be exploited in magnetic resonance imaging (MRI), hyperthermia or magnetic targeting [12] thereby increasing the array of biomedical applications of liposomal structures [13].…”

Section: Introductionmentioning

confidence: 99%

Addressing the problem of cationic lipid-mediated toxicity: The magnetoliposome model

2009

Self Cite

View full text Add to dashboard Cite

“…ML's have received special attention because they can be guided or localized in a specific target by external magnetic fields. Therefore, ML's have great potential applications in the field of modern biotechnology and biomedicine, such as drug-delivery carriers, magnetic resonance imaging markers for cancer diagnosis, and thermal cancer therapy [5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Biodistribution and biocompatibility investigation in magnetoliposome treated mice

Lacava

Garcia

Lacava

et al. 2004

Journal of Spectroscopy

View full text Add to dashboard Cite

Magnetoliposomes (ML's) may be successfully applied for several purposes. A dimyristoylphosphatidyl‒choline‒based ML (ML‒2) sample was developed as a precursor of more complex thermal cancer therapy systems. The present study reports on morphology and magnetic resonance (MR) investigations carried out with the magnetite‒based ML‒2 sample. For the experiments, adult female Swiss mice were endovenously treated with a bolus dose of 100 µl of ML‒2. Morphology and room‒temperature MR studies (X‒band experiments) were performed in several organs collected from 1 hour to 28 days after ML administration. Histological data showed magnetic nanoparticle (MNP) clusters up to the 28th day in the liver and spleen tissues. In spite of the presence of MNP clusters, no morphological alterations were observed, supporting the biocompatibility of the ML‒2. MR signal was detected only in the liver and spleen tissues and showed that the MNP's concentration was not altered from 48 hours to 28 days after ML injection. Using MR data, important pharmacokinetic parameters, such as the effective clearance (half‒life) and peak concentration, were obtained for the liver and spleen.

show abstract

Catalytic durability of magnetoproteoliposomes captured by high-gradient magnetic forces in a miniature fixed-bed reactor

Cited by 15 publications

References 22 publications

Novel support for membrane enzyme immobilization: gel beads containing polymerized phospholipid vesicles

Novel support for membrane enzyme immobilization: gel beads containing polymerized phospholipid vesicles

Addressing the problem of cationic lipid-mediated toxicity: The magnetoliposome model

Biodistribution and biocompatibility investigation in magnetoliposome treated mice

Contact Info

Product

Resources

About