A novel series of ferrocenyl surfactants was synthesized by the reaction of ferrocene disulfonic acid with different primary and tertiary fatty amines to produce the corresponding ammonium salts Fc[SO 3 -? NH 3 (CH 2 ) n CH 3 ] 2 , where n = 9, 11, or 15 and Fc[SO 3 -? NH(CH 3 ) 2 (CH 2 ) n CH 3 ] 2 , where n = 7 or 11, respectively, and where Fc = ferrocene. Chemical structures were confirmed by microelemental analysis, FTIR, and 1 H NMR spectroscopy. The critical micelle concentration of each prepared surfactant was determined using equilibrium surface tension. Furthermore, air/water interface parameters including effectiveness (p CMC ), efficiency (Pc 20 ), maximum surface excess (U max ), and minimum surface area (A min ) were determined at 30, 40, and 50°C. Thermodynamic parameters (DG°, DS°, and DH°) for both micellization and adsorption processes were recorded. The new synthesized surfactants were screened as antimicrobial agents against different bacterial and fungal organisms.