Catalase‐Mimetic Artificial Biocatalysts with Ru Catalytic Centers for ROS Elimination and Stem‐Cell Protection

Abstract

Exploring high‐efficiency reactive oxygen species (ROS)‐elimination materials is of great importance for combating oxidative stress in diverse diseases, especially stem‐cell‐based biotherapeutics. By mimicking the FeN active centers of natural catalase, here, an innovative concept to design ROS‐elimination artificial biocatalysts with Ru catalytic centers for stem‐cell protection is reported. The experimental studies and theoretical calculations have systematically disclosed the activity merits and structure diversities of different Ru sites when serving as ROS‐elimination artificial biocatalysts. Benefiting from the metallic electronic structures and synergetic effects of multiple sites, the artificial biocatalysts with Ru cluster centers present exceptional ROS‐elimination activity; notably, it shows much higher catalytic efficiency per Ru atom on decomposing H2O2 when compared to the isolated single‐atom Ru sites, which is more efficient than that of the natural antioxidants and recently reported state‐of‐the‐art ROS‐scavenging biocatalysts. The systematic stem‐cell protection studies reveal that the catalase‐like artificial biocatalysts can provide efficient rescue ability for survival, adhesion, and differentiation functions of human mesenchymal stem cells in high ROS level conditions. It is suggested that applying these artificial biocatalysts with Ru cluster centers will offer a new pathway for engineering high‐performance ROS‐scavenging materials in stem‐cell‐based therapeutics and many other ROS‐related diseases.

Amorphizing Metal Selenides-Based ROS Biocatalysts at Surface Nanolayer toward Ultrafast Inflammatory Diabetic Wound Healing

“…In recent years, given the enormous benefits of antioxidase-like biocatalysts, such as efficient biocatalytic activity to preserve redox balance, low antigenicity, high stability after administration, and large-scale production, , there has been dramatically increased interest in designing biocatalysts via diverse transition metal compounds and metal nanoparticles that can mimic the function of natural antioxidases, including catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD). − Despite thrilling progress in discovering catalytic ROS-scavenging materials, , the development of ultrafast and broad-spectrum antioxidase-like catalytic structures is still a grand challenge. They are mostly based on metal compounds, such as Co 3 O 4 , MnO 2 , CeO 2 , , and Pt-doped CeO 2 , due to their high biodegradability and good biocompatibility.…”

Antioxidase‐Like Nanobiocatalysts with Ultrafast and Reversible Redox‐Centers to Secure Stem Cells and Periodontal Tissues

“…[ 8,9 ] In recent years, thrilling innovations to engineer artificial biocatalysts with diverse metal coordination environments that can mimic the function of natural enzymes have been promoted by the spectacularly increasing demands in enzyme‐like nanomedicines. [ 10–16 ] Nevertheless, most of the current studies on catalytic biotherapeutics focus on the synthesis, characterization, and biological applications of ROS‐production materials. [ 17–26 ] The developments of ROS‐scavenging biocatalysts are very slow and challenging.…”

Semiconducting Titanate Supported Ruthenium Clusterzymes for Ultrasound‐Amplified Biocatalytic Tumor Nanotherapies

“…Here, by taking the advantages of semiconducting and biocompatible barium titanate (BaTiO 3 , BTO) [52][53][54] and superior catalytic activities of Ru species in multielectron reactions, [55][56][57][58][59] we report synthesizing a new BTO-supported Ru clusterzymes (namely RuNC/BTO) via a facile method for ultrasound-amplified biocatalytic tumor nanotherapies. The morphology and chemical/electronic structures of the RuNC/ BTO are analyzed, and our data prove that RuNC/BTO consists of abundant surface-decorated Ru nanoclusters via RuO coordination.…”