Catalase Is Differentially Expressed in Dividing and Nondividing Protoplasts

Siminis, C. I.; Kanellis, Angelos K.; Roubelakis‐Angelakis, Kalliopi A.

doi:10.1104/pp.105.4.1375

Cited by 59 publications

(43 citation statements)

References 16 publications

(10 reference statements)

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“…These observations correspond with previous studies by Siminis et al (1994) and Agrawal and Purohit (2012), who reported that early stages of regeneration were accompanied by a high content of endogenous H 2 O 2 . The results presented here stay in agreement with previous findings confirming the hypothesis that auxin together with the optimal oxidative status act together to set the asymmetry of mother cells, which is a necessary condition for generation of daughter cells and beginning of new developmental pathway (Livanos et al 2016;Libik-Konieczny et al 2014;Nick 2016).…”

Section: Prooxidants Strongly Affect Adventitious Roots Formation Andsupporting

confidence: 81%

“…Whilst transient increase of H 2 O 2 seems to be necessary for initiation of in vitro morphogenesis (Siminis et al 1994;Kairong et al 1999;Agrawal and Purohit 2012; data presented here), the long-lasting overproduction of H 2 O 2 was shown to cause severe oxidative stress which inhibits root formation (Li et al 2009a;Pal Singh et al 2009). In our study, the non-rhizogenic hypocotyls were characterized by low H 2 O 2 content at day 3 of culture and relatively high steady-state level at day 7 and 14.…”

Section: Prooxidants Strongly Affect Adventitious Roots Formation Andmentioning

confidence: 99%

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Influence of anti- and prooxidants on rhizogenesis from hypocotyls of Mesembryanthemum crystallinum L. cultured in vitro

Libik-Konieczny

Kozieradzka-Kiszkurno

Michalec-Warzecha

et al. 2017

Acta Physiol Plant

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The enrichment with antioxidants (glutathione or ascorbate) or prooxidants (alloxan, methylviologen, hydrogen peroxide) of root inducing medium significantly decreased rhizogenesis frequency (alloxan, hydrogen peroxide) or inhibited roots regeneration (ascorbate, methylviologen) during the in vitro culture of Mesembryanthemum crystallinum L. hypocotyls. The adventitious roots morphology, root hairs length and density, was also influenced. Changes in the rhizogenesis course were related to the differences in hydrogen peroxide concentration during following days of culture between explants exhibiting morphogenic potential and those without the ability to form adventitious roots. In explants with morphogenic potential, rhizogenesis induction was always accompanied by a high level of hydrogen peroxide followed by the decrease in H 2 O 2 content in following days. In contrast, in the explants without regeneration potential, the level of hydrogen peroxide was increasing during the culture period. Activity patterns of superoxide dismutase (SOD) and guaiacol peroxidase (POX) in the following days of culture were similar in the explants exhibiting regeneration potential cultured on different media. Total activity of SOD decreased during initial days of culture and then increased due to the activation of additional SOD isoform described as MnSODII. The activity of POX was low during the rhizogenesis induction, and then increased during following days of culture; the increase was correlated with the decrease in hydrogen peroxide content. In the explants without the ability to regenerate roots, the total activity of SOD was low throughout the whole culture period, whereas the POX activity was significantly higher than in hypocotyls with regeneration potential. It might be concluded that the increase in hydrogen peroxide during initial stages of rhizogenesis and the induction of MnSODII are prerequisites for adventitious roots formation from hypocotyls of M. crystallinum, independently in the presence of anti-or prooxidant in the culture medium.

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Section: Prooxidants Strongly Affect Adventitious Roots Formation Andsupporting

confidence: 81%

Section: Prooxidants Strongly Affect Adventitious Roots Formation Andmentioning

confidence: 99%

Influence of anti- and prooxidants on rhizogenesis from hypocotyls of Mesembryanthemum crystallinum L. cultured in vitro

Libik-Konieczny

Kozieradzka-Kiszkurno

Michalec-Warzecha

et al. 2017

Acta Physiol Plant

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“…We have thoroughly studied intra-and extracellular H 2 O 2 and O 2 .2 generation, accumulation, and scavenging in plant protoplasts (Siminis et al, 1994;de Marco and Roubelakis-Angelakis, 1996;Roubelakis-Angelakis, 1999, 2005;Papadakis et al, 2001). H 2 O 2 generation in the apoplast can be ascribed to oxalate oxidases, O 2 .2 dismutation (Papadakis et al, 2001), peroxidases (POXs; de Marco and Roubelakis- Angelakis, 1996), or amine oxidases (Laurenzi et al, 1999;Sebela et al, 2001;Rea et al, 2002;Papadakis and Roubelakis-Angelakis, 2005).…”

mentioning

confidence: 99%

Sites and Regulation of Polyamine Catabolism in the Tobacco Plant. Correlations with Cell Division/Expansion, Cell Cycle Progression, and Vascular Development

2005

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We previously gave a picture of the homeostatic characteristics of polyamine (PA) biosynthesis and conjugation in tobacco (Nicotiana tabacum) plant organs during development. In this work, we present the sites and regulation of PA catabolism related to cell division/expansion, cell cycle progression, and vascular development in the tobacco plant. Diamine oxidase (DAO), PA oxidase (PAO), peroxidases (POXs), and putrescine N-methyltransferase expressions follow temporally and spatially discrete patterns in shoot apical cells, leaves (apical, peripheral, and central regions), acropetal and basipetal petiole regions, internodes, and young and old roots in developing plants. DAO and PAO produce hydrogen peroxide, a plant signal molecule and substrate for POXs. Gene expression and immunohistochemistry analyses reveal that amine oxidases in developing tobacco tissues precede and overlap with nascent nuclear DNA and also with POXs and lignification. In mature and old tissues, flow cytometry indicates that amine oxidase and POX activities, as well as pao gene and PAO protein levels, coincide with G2 nuclear phase and endoreduplication. In young versus the older roots, amine oxidases and POX expression decrease with parallel inhibition of G2 advance and endoreduplication, whereas putrescine N-methyltransferase dramatically increases. In both hypergeous and hypogeous tissues, DAO and PAO expression occurs in cells destined to undergo lignification, suggesting a different in situ localization. DNA synthesis early in development and the advance in cell cycle/endocycle are temporally and spatially related to PA catabolism and vascular development.Polyamines (PAs) are abundant DNA-and RNAbinding organic cations. They have been correlated with specific roles in embryonic development (Kusunoki and Yasumasu, 1978), carcinogenesis and immune system functions (Seiler et al., 1998), defense (Schroder et al., 1998), antibiotic potency (Petropoulos et al., 2004), cell proliferation and apoptosis (for review, see Wallace et al., 2003;Janne et al., 2004), and in many biotic and abiotic stresses (Jokela et al., 1997;Kurepa et al., 1998;Perez-Amador et al., 2002; for review, see Bouchereau et al., 1999). Investigations on PA physiological functions have been focused mainly on changes in their levels and spectra, leaving the biological significance to be determined. Although genetic analyses have been conducted at the gene level (Laurenzi et al., 1999;Sebela et al., 2001;Rea et al., 2002;Papadakis and Roubelakis-Angelakis, 2005). H 2 O 2 produced by the action of diamine oxidases (DAOs) and PA oxidases (PAOs) can be utilized by POX enzymes in wallstiffening reactions during cell growth and differentiation (Wisniewski et al., 2000). In alkaloid producing plants, such as tobacco (Nicotiana tabacum), putrescine (Put), apart from serving as substrate for spermidine (Spd) biosynthesis or for oxidation (Bagni and Tassoni, 2001), is N-methylated by Put N-methyltransferase (PMT), which catalyzes the first committed steps in the biosynthesis of n...

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“…CPW salt solution contains inorganic ions that play a role in plasma membrane stabilization and, as revealed by our study, induce, in some way, the antioxidant machinery of the cell. In turn, a high activity of antioxidant system is most probably crucial for the development of protoplasts in culture as well as for the regeneration capacity of protoplasts (Siminis et al, 1993(Siminis et al, , 1994Xu et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Activity of selected components of antioxidant system in grass pea and yellow lupine protoplasts after enzymatic isolation

Wiszniewska¹,

Piwowarczyk²

2015

bta

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In this study, the performance of the antioxidant system in protoplasts of grass pea (Lathyrus sativus L.) and yellow lupine (Lupinus luteus L.) isolated using solutions of different compositions was evaluated. Protoplasts were isolated from leaves of grass pea and cotyledons of yellow lupine seedlings grown in vitro. Hydrolytic enzymes were dissolved in three different solutions (ultrapure water, inorganic salt solution (CPW), and inorganic salt solution with organic additives (C)). Protoplasts purified using these solutions were subjected to the analyses of peroxidase (POD) and radical scavenging activities. Phenolic profile was also determined. In grass pea, the highest POD was determined in protoplasts isolated in CPW salt solution, while in yellow lupine, the other two solutions were found superior. Protoplasts isolated in CPW salt solution had the highest content of phenolic compounds, ranging from 28.8 to 40.1 and from 32.0 to 58.0 mg/g fresh weight (f.w.) in lupine and grass pea, respectively. Moreover, in protoplasts obtained using CPW solution, the total antioxidant activity was the highest in all tested genotypes as expressed by high proportion of scavenged 2,2,-diphenyl-1-picrylhydrazyl (DPPH) radical. The results showed that changes in composition of the isolation solutions affected the level and activity of selected components belonging to cell antioxidant system in legume protoplasts. Considering the recalcitrance of two studied plants in protoplast culture, this finding is an important piece of information because events occurring at the stage of isolation may affect further development of protoplasts in the culture.

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Catalase Is Differentially Expressed in Dividing and Nondividing Protoplasts

Cited by 59 publications

References 16 publications

Influence of anti- and prooxidants on rhizogenesis from hypocotyls of Mesembryanthemum crystallinum L. cultured in vitro

Influence of anti- and prooxidants on rhizogenesis from hypocotyls of Mesembryanthemum crystallinum L. cultured in vitro

Sites and Regulation of Polyamine Catabolism in the Tobacco Plant. Correlations with Cell Division/Expansion, Cell Cycle Progression, and Vascular Development

Activity of selected components of antioxidant system in grass pea and yellow lupine protoplasts after enzymatic isolation

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